################################## # NO CUT ENZYMES ( 45 enzymes) # ################################## AclI AflIII AgeI Aor51HI AscI AviII BclI BsiWI BspDI BspHI BspLU11I BssHII Bst1107I BstBI BstZ17I ClaI DrdI Eco47III EcoRV EcoT22I FbaI FseI FspI HindIII HpaI MluI NdeI NheI NotI NruI NsiI NspV PacI PinAI PmeI Psp1406I PvuI RcaI SapI ScaI SfuI SgrAI SnaBI SpeI SwaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AatII 856 GACGTC GACGT^C Acc65I 6188 GGTACC G^GTACC AccI 4893 GTCGAC GT^[AC][GT]AC AflII 6290 CTTAAG C^TTAAG AhdI 4912 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 5449 GTGCAC G^TGCAC ApaLI 5449 GTGCAC G^TGCAC AseI 6314 ATTAAT AT^TAAT AsnI 6314 ATTAAT AT^TAAT Asp718I 6188 GGTACC G^GTACC AspEI 4912 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 717 CCTAGG C^CTAGG BbrPI 1901 CACGTG CAC^GTG BbvCI 5418 CCTCAGC CCTCAGC(-5/-2) BfrI 6290 CTTAAG C^TTAAG BglII 4691 AGATCT A^GATCT BlnI 717 CCTAGG C^CTAGG Bsp1407I 6861 TGTACA T^GTACA BsrDI 3086 GCAATG GCAATG(2/0) BsrGI 6861 TGTACA T^GTACA BssSI 5411 CTCGTG CACGAG(-5/-1) BstAPI 3549 GCAGGGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 237 CCAGCAGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CpoI 4164 CGGACCG CG^G[AT]CCG DraI 2314 TTTAAA TTT^AAA DraIII 5521 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 1667 CGGCCG C^GGCCG Eam1105I 4912 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 1667 CGGCCG C^GGCCG Eco52I 1667 CGGCCG C^GGCCG HincII 4893 GTCGAC GT[CT]^[GA]AC HindII 4893 GTCGAC GT[CT]^[GA]AC KpnI 6188 GGTACC GGTAC^C MfeI 2072 CAATTG C^AATTG MunI 2072 CAATTG C^AATTG PmaCI 1901 CACGTG CAC^GTG PmlI 1901 CACGTG CAC^GTG PshBI 6314 ATTAAT AT^TAAT RsrII 4164 CGGACCG CG^G[AT]CCG SalI 4893 GTCGAC G^TCGAC SbfI 2989 CCTGCAGG CCTGCA^GG SexAI 4008 ACCAGGT A^CC[AT]GGT Sse8387I 2989 CCTGCAGG CCTGCA^GG SspBI 6861 TGTACA T^GTACA XbaI 6225 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### Asp700I 4934 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 7002 GAACCCTTTC AspI 1685 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 7429 GACTGCGTC BbeI 2041 GGCGCC GGCGC^C 2536 GGCGCC BcgI 1253 GCATGCTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 5423 GCATGCCATTCG BlpI 108 GCTCAGC GC^T[ACGT]AGC 5061 GCTGAGC Bpu1102I 108 GCTCAGC GC^T[ACGT]AGC 5061 GCTGAGC BsaBI 456 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 6928 GATTCCCATC CelII 108 GCTCAGC GC^T[ACGT]AGC 5061 GCTGAGC EcoRI 3167 GAATTC G^AATTC 4978 GAATTC KasI 2041 GGCGCC G^GCGCC 2536 GGCGCC MamI 456 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 6928 GATTCCCATC NarI 2041 GGCGCC GG^CGCC 2536 GGCGCC PaeR7I 1640 CTCGAG C^TCGAG 2234 CTCGAG PflFI 1685 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 7429 GACTGCGTC SfoI 2041 GGCGCC GGC^GCC 2536 GGCGCC SphI 1253 GCATGC GCATG^C 5423 GCATGC SspI 644 AATATT AAT^ATT 5961 AATATT Tth111I 1685 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 7429 GACTGCGTC XhoI 1640 CTCGAG C^TCGAG 2234 CTCGAG XmnI 4934 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 7002 GAACCCTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 148 enzymes) # ######################################### AccII AccIII AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI ApaI ApoI AspHI AvaI AvaII BalI BamHI BanI BanII BbsI BbvI BciVI BcnI BfaI BglI BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BspEI BspMI BsrBI BsrFI BsrI BssKI BstEII BstF5I BstNI BstPI BstUI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MroI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PshAI PstI PvuII RsaI SacI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SfiI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI