##################################
# NO CUT ENZYMES ( 45 enzymes) #
##################################
AclI AflIII AgeI Aor51HI AscI
AviII BclI BsiWI BspDI BspHI
BspLU11I BssHII Bst1107I BstBI BstZ17I
ClaI DrdI Eco47III EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
MluI NdeI NheI NotI NruI
NsiI NspV PacI PinAI PmeI
Psp1406I PvuI RcaI SapI ScaI
SfuI SgrAI SnaBI SpeI SwaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 856 GACGTC GACGT^C
Acc65I 6188 GGTACC G^GTACC
AccI 4893 GTCGAC GT^[AC][GT]AC
AflII 6290 CTTAAG C^TTAAG
AhdI 4912 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 5449 GTGCAC G^TGCAC
ApaLI 5449 GTGCAC G^TGCAC
AseI 6314 ATTAAT AT^TAAT
AsnI 6314 ATTAAT AT^TAAT
Asp718I 6188 GGTACC G^GTACC
AspEI 4912 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 717 CCTAGG C^CTAGG
BbrPI 1901 CACGTG CAC^GTG
BbvCI 5418 CCTCAGC CCTCAGC(-5/-2)
BfrI 6290 CTTAAG C^TTAAG
BglII 4691 AGATCT A^GATCT
BlnI 717 CCTAGG C^CTAGG
Bsp1407I 6861 TGTACA T^GTACA
BsrDI 3086 GCAATG GCAATG(2/0)
BsrGI 6861 TGTACA T^GTACA
BssSI 5411 CTCGTG CACGAG(-5/-1)
BstAPI 3549 GCAGGGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 237 CCAGCAGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 4164 CGGACCG CG^G[AT]CCG
DraI 2314 TTTAAA TTT^AAA
DraIII 5521 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1667 CGGCCG C^GGCCG
Eam1105I 4912 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1667 CGGCCG C^GGCCG
Eco52I 1667 CGGCCG C^GGCCG
HincII 4893 GTCGAC GT[CT]^[GA]AC
HindII 4893 GTCGAC GT[CT]^[GA]AC
KpnI 6188 GGTACC GGTAC^C
MfeI 2072 CAATTG C^AATTG
MunI 2072 CAATTG C^AATTG
PmaCI 1901 CACGTG CAC^GTG
PmlI 1901 CACGTG CAC^GTG
PshBI 6314 ATTAAT AT^TAAT
RsrII 4164 CGGACCG CG^G[AT]CCG
SalI 4893 GTCGAC G^TCGAC
SbfI 2989 CCTGCAGG CCTGCA^GG
SexAI 4008 ACCAGGT A^CC[AT]GGT
Sse8387I 2989 CCTGCAGG CCTGCA^GG
SspBI 6861 TGTACA T^GTACA
XbaI 6225 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
Asp700I 4934 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
7002 GAACCCTTTC
AspI 1685 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
7429 GACTGCGTC
BbeI 2041 GGCGCC GGCGC^C
2536 GGCGCC
BcgI 1253 GCATGCTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5423 GCATGCCATTCG
BlpI 108 GCTCAGC GC^T[ACGT]AGC
5061 GCTGAGC
Bpu1102I 108 GCTCAGC GC^T[ACGT]AGC
5061 GCTGAGC
BsaBI 456 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
6928 GATTCCCATC
CelII 108 GCTCAGC GC^T[ACGT]AGC
5061 GCTGAGC
EcoRI 3167 GAATTC G^AATTC
4978 GAATTC
KasI 2041 GGCGCC G^GCGCC
2536 GGCGCC
MamI 456 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
6928 GATTCCCATC
NarI 2041 GGCGCC GG^CGCC
2536 GGCGCC
PaeR7I 1640 CTCGAG C^TCGAG
2234 CTCGAG
PflFI 1685 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
7429 GACTGCGTC
SfoI 2041 GGCGCC GGC^GCC
2536 GGCGCC
SphI 1253 GCATGC GCATG^C
5423 GCATGC
SspI 644 AATATT AAT^ATT
5961 AATATT
Tth111I 1685 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
7429 GACTGCGTC
XhoI 1640 CTCGAG C^TCGAG
2234 CTCGAG
XmnI 4934 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
7002 GAACCCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 148 enzymes) #
#########################################
AccII AccIII AciI AcsI AcyI
AfaI AluI AlwI AlwNI AocI
ApaI ApoI AspHI AvaI AvaII
BalI BamHI BanI BanII BbsI
BbvI BciVI BcnI BfaI BglI
BmyI BpmI BpuAI BsaAI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I BspEI BspMI BsrBI BsrFI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstUI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MroI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PshAI
PstI PvuII RsaI SacI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SfiI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI