##################################
# NO CUT ENZYMES ( 42 enzymes) #
##################################
AflII AgeI AscI AseI AsnI
BclI BfrI BsaBI BsiWI BssHII
BssSI Bst1107I BstBI BstZ17I CpoI
DraI EcoRI FbaI FseI HpaI
MamI MfeI MluI MunI NdeI
NheI NotI NruI NspV PacI
PinAI PmeI PshBI RsrII SalI
SfiI SfuI SgrAI SnaBI SpeI
SspI SwaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AclI 6320 AACGTT AA^CGTT
Aor51HI 1263 AGCGCT AGC^GCT
Asp700I 5329 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 4119 TGCGCA TGC^GCA
AvrII 117 CCTAGG C^CTAGG
BamHI 2916 GGATCC G^GATCC
BciVI 362 GGATAC GTATCC(6/5)
BlnI 117 CCTAGG C^CTAGG
Bsp1407I 348 TGTACA T^GTACA
BspLU11I 8351 ACATGT A^CATGT
BsrGI 348 TGTACA T^GTACA
DrdI 515 GACCAAAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2050 CGGCCG C^GGCCG
EclXI 2050 CGGCCG C^GGCCG
Eco47III 1263 AGCGCT AGC^GCT
Eco52I 2050 CGGCCG C^GGCCG
EcoRV 8435 GATATC GAT^ATC
FspI 4119 TGCGCA TGC^GCA
HindIII 2434 AAGCTT A^AGCTT
NaeI 3807 GCCGGC GCC^GGC
NgoMIV 3807 GCCGGC G^CCGGC
PshAI 7622 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 6320 AACGTT AA^CGTT
PvuI 2805 CGATCG CGAT^CG
SapI 883 GAAGAGC GCTCTTC(1/4)
SbfI 7281 CCTGCAGG CCTGCA^GG
SphI 7944 GCATGC GCATG^C
Sse8387I 7281 CCTGCAGG CCTGCA^GG
SspBI 348 TGTACA T^GTACA
XbaI 556 TCTAGA T^CTAGA
XmnI 5329 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 497 GGTACC G^GTACC
4947 GGTACC
AccI 5009 GTCTAC GT^[AC][GT]AC
6821 GTCTAC
AhdI 6439 GACGTATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
7724 GACTCCCAGTC
AocI 1193 CCTGAGG CC^T[ACGT]AGG
3819 CCTCAGG
Asp718I 497 GGTACC G^GTACC
4947 GGTACC
AspEI 6439 GACGTATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
7724 GACTCCCAGTC
BbeI 2069 GGCGCC GGCGC^C
4996 GGCGCC
BglII 2340 AGATCT A^GATCT
7035 AGATCT
BsaI 6011 GAGACC GGTCTC(1/5)
6283 GGTCTC
BspDI 617 ATCGAT AT^CGAT
7988 ATCGAT
BspHI 3348 TCATGA T^CATGA
8172 TCATGA
BsrFI 3075 GCCGGT [GA]^CCGG[CT]
3807 GCCGGC
Bsu36I 1193 CCTGAGG CC^T[ACGT]AGG
3819 CCTCAGG
Cfr10I 3075 GCCGGT [GA]^CCGG[CT]
3807 GCCGGC
ClaI 617 ATCGAT AT^CGAT
7988 ATCGAT
Eam1105I 6439 GACGTATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
7724 GACTCCCAGTC
Eco81I 1193 CCTGAGG CC^T[ACGT]AGG
3819 CCTCAGG
EcoNI 2980 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5146 CCTCATCCAGG
EcoT22I 5552 ATGCAT ATGCA^T
6338 ATGCAT
KasI 2069 GGCGCC G^GCGCC
4996 GGCGCC
KpnI 497 GGTACC GGTAC^C
4947 GGTACC
MseI 693 TTAA T^TAA
6000 TTAA
NarI 2069 GGCGCC GG^CGCC
4996 GGCGCC
NsiI 5552 ATGCAT ATGCA^T
6338 ATGCAT
PaeR7I 1290 CTCGAG C^TCGAG
2555 CTCGAG
RcaI 3348 TCATGA T^CATGA
8172 TCATGA
ScaI 4821 AGTACT AGT^ACT
8247 AGTACT
SfoI 2069 GGCGCC GGC^GCC
4996 GGCGCC
Tru9I 693 TTAA T^TAA
6000 TTAA
XhoI 1290 CTCGAG C^TCGAG
2555 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 155 enzymes) #
#########################################
AatII AccII AccIII AciI AcsI
AcyI AfaI AflIII AluI Alw44I
AlwI AlwNI ApaI ApaLI ApoI
AspHI AspI AvaI AvaII BalI
BanI BanII BbrPI BbsI BbvCI
BbvI BcgI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaJI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrI BssKI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MroI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflFI PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
SacI SacII Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmaI SmlI
StuI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XcmI XhoII XmaCI XmaI