##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AccIII AclI AflII AgeI AhdI
AscI AspEI AviII BcgI BclI
BfrI BglII BseAI BsiWI Bsp1407I
BspEI BsrGI Bst1107I BstBI BstZ17I
Eam1105I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HpaI
KspI MfeI MluI MroI MunI
NaeI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI Psp1406I PvuI SacII SalI
ScaI SfiI SfuI SgrAI SphI
SspBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 711 GGTACC G^GTACC
Aor51HI 192 AGCGCT AGC^GCT
Asp718I 711 GGTACC G^GTACC
AspI 4680 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 592 GGCGCC GGCGC^C
BbrPI 6073 CACGTG CAC^GTG
BbvCI 1196 GCTGAGG CCTCAGC(-5/-2)
BciVI 3135 GTATCC GTATCC(6/5)
BsaWI 182 ACCGGA [AT]^CCGG[AT]
BsiEI 3716 CGGCCG CG[GA][CT]^CG
BspDI 2712 ATCGAT AT^CGAT
BspLU11I 5454 ACATGT A^CATGT
BsrFI 303 GCCGGT [GA]^CCGG[CT]
BstEII 3930 GGTAACC G^GT[ACGT]ACC
BstPI 3930 GGTAACC G^GT[ACGT]ACC
Cfr10I 303 GCCGGT [GA]^CCGG[CT]
ClaI 2712 ATCGAT AT^CGAT
CpoI 305 CGGTCCG CG^G[AT]CCG
DraIII 4615 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4992 GACTCTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3716 CGGCCG C^GGCCG
EclXI 3716 CGGCCG C^GGCCG
Eco47III 192 AGCGCT AGC^GCT
Eco52I 3716 CGGCCG C^GGCCG
EcoO65I 3930 GGTAACC G^GT[ACGT]ACC
HgaI 324 GCGTC GACGC(5/10)
KasI 592 GGCGCC G^GCGCC
KpnI 711 GGTACC GGTAC^C
NarI 592 GGCGCC GG^CGCC
PflFI 4680 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 6073 CACGTG CAC^GTG
PmlI 6073 CACGTG CAC^GTG
PshAI 2367 GACCCGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 305 CGGTCCG CG^G[AT]CCG
SbfI 1612 CCTGCAGG CCTGCA^GG
SfoI 592 GGCGCC GGC^GCC
SnaBI 7202 TACGTA TAC^GTA
SpeI 4633 ACTAGT A^CTAGT
Sse8387I 1612 CCTGCAGG CCTGCA^GG
Tth111I 4680 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AatII 583 GACGTC GACGT^C
4269 GACGTC
AocI 2534 CCTGAGG CC^T[ACGT]AGG
5931 CCTGAGG
AseI 5977 ATTAAT AT^TAAT
6428 ATTAAT
AsnI 5977 ATTAAT AT^TAAT
6428 ATTAAT
Asp700I 4064 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5572 GAAGACCTTC
AvrII 2772 CCTAGG C^CTAGG
5939 CCTAGG
BamHI 2783 GGATCC G^GATCC
5841 GGATCC
BglI 35 GCCGTAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3998 GCCTATAGGGC
BlnI 2772 CCTAGG C^CTAGG
5939 CCTAGG
BspHI 913 TCATGA T^CATGA
2978 TCATGA
BssHII 260 GCGCGC G^CGCGC
262 GCGCGC
BssSI 147 CTCGTG CACGAG(-5/-1)
874 CTCGTG
BstXI 819 CCACCCCGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1581 CCACCAAGATGG
Bsu36I 2534 CCTGAGG CC^T[ACGT]AGG
5931 CCTGAGG
Eco81I 2534 CCTGAGG CC^T[ACGT]AGG
5931 CCTGAGG
PshBI 5977 ATTAAT AT^TAAT
6428 ATTAAT
RcaI 913 TCATGA T^CATGA
2978 TCATGA
SacI 2901 GAGCTC GAGCT^C
4367 GAGCTC
SapI 4174 GCTCTTC GCTCTTC(1/4)
5292 GCTCTTC
SexAI 1854 ACCTGGT A^CC[AT]GGT
2603 ACCTGGT
StuI 5147 AGGCCT AGG^CCT
5426 AGGCCT
XbaI 1396 TCTAGA T^CTAGA
6550 TCTAGA
XmnI 4064 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5572 GAAGACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI Alw44I AlwI
AlwNI ApaI ApaLI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NheI NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI