##################################
# NO CUT ENZYMES ( 45 enzymes) #
##################################
AatII AclI AcyI AflIII AhdI
AscI AspEI BbeI BsaHI BsiWI
BsmBI BspDI BspHI BspLU11I BssSI
ClaI DrdI Eam1105I FseI Hin1I
HpaI KasI KspI MfeI MluI
MunI NarI NotI NruI PacI
PmeI Psp1406I PvuI RcaI SacII
SalI SapI SbfI ScaI SfoI
SgrAI SpeI Sse8387I SwaI XbaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 7496 GTATAC GT^[AC][GT]AC
AccIII 2950 TCCGGA T^CCGGA
AflII 7116 CTTAAG C^TTAAG
AgeI 1661 ACCGGT A^CCGGT
Aor51HI 4328 AGCGCT AGC^GCT
AspI 4687 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 5522 CCTAGG C^CTAGG
BamHI 1599 GGATCC G^GATCC
BbrPI 508 CACGTG CAC^GTG
BclI 2505 TGATCA T^GATCA
BfrI 7116 CTTAAG C^TTAAG
BlnI 5522 CCTAGG C^CTAGG
BsaBI 687 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2950 TCCGGA T^CCGGA
BspEI 2950 TCCGGA T^CCGGA
BssHII 3245 GCGCGC G^CGCGC
Bst1107I 7496 GTATAC GTA^TAC
BstBI 4822 TTCGAA TT^CGAA
BstZ17I 7496 GTATAC GTA^TAC
CpoI 4733 CGGACCG CG^G[AT]CCG
EagI 4134 CGGCCG C^GGCCG
EclXI 4134 CGGCCG C^GGCCG
Eco47III 4328 AGCGCT AGC^GCT
Eco52I 4134 CGGCCG C^GGCCG
EcoRI 4009 GAATTC G^AATTC
FbaI 2505 TGATCA T^GATCA
MamI 687 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2950 TCCGGA T^CCGGA
NdeI 7760 CATATG CA^TATG
NheI 3961 GCTAGC G^CTAGC
NspV 4822 TTCGAA TT^CGAA
PaeR7I 1277 CTCGAG C^TCGAG
PflFI 4687 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1661 ACCGGT A^CCGGT
PmaCI 508 CACGTG CAC^GTG
PmlI 508 CACGTG CAC^GTG
PshAI 2031 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 4733 CGGACCG CG^G[AT]CCG
SexAI 1103 ACCAGGT A^CC[AT]GGT
SfiI 4938 GGCCCACATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 4822 TTCGAA TT^CGAA
SnaBI 7023 TACGTA TAC^GTA
Tth111I 4687 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1277 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 627 GGTACC G^GTACC
2569 GGTACC
AseI 7074 ATTAAT AT^TAAT
7623 ATTAAT
AsnI 7074 ATTAAT AT^TAAT
7623 ATTAAT
Asp700I 4825 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5853 GAAAATATTC
Asp718I 627 GGTACC G^GTACC
2569 GGTACC
AviII 374 TGCGCA TGC^GCA
3193 TGCGCA
BcgI 427 GCACCACAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
445 GCACCACAGTCG
BciVI 2236 GGATAC GTATCC(6/5)
5919 GGATAC
BlpI 4534 GCTCAGC GC^T[ACGT]AGC
5246 GCTTAGC
Bpu1102I 4534 GCTCAGC GC^T[ACGT]AGC
5246 GCTTAGC
BsaWI 1661 ACCGGT [AT]^CCGG[AT]
2950 TCCGGA
BsiEI 1663 CGGTCG CG[GA][CT]^CG
4134 CGGCCG
Bsp1407I 779 TGTACA T^GTACA
4082 TGTACA
BsrDI 5273 CATTGC GCAATG(2/0)
6423 GCAATG
BsrGI 779 TGTACA T^GTACA
4082 TGTACA
BstEII 2148 GGTGACC G^GT[ACGT]ACC
2760 GGTCACC
BstPI 2148 GGTGACC G^GT[ACGT]ACC
2760 GGTCACC
CelII 4534 GCTCAGC GC^T[ACGT]AGC
5246 GCTTAGC
EcoO65I 2148 GGTGACC G^GT[ACGT]ACC
2760 GGTCACC
EcoT22I 7379 ATGCAT ATGCA^T
7425 ATGCAT
FspI 374 TGCGCA TGC^GCA
3193 TGCGCA
HincII 3337 GTCAAC GT[CT]^[GA]AC
4264 GTCAAC
HindII 3337 GTCAAC GT[CT]^[GA]AC
4264 GTCAAC
KpnI 627 GGTACC GGTAC^C
2569 GGTACC
NsiI 7379 ATGCAT ATGCA^T
7425 ATGCAT
PshBI 7074 ATTAAT AT^TAAT
7623 ATTAAT
SacI 1836 GAGCTC GAGCT^C
4518 GAGCTC
SphI 5422 GCATGC GCATG^C
7427 GCATGC
SspBI 779 TGTACA T^GTACA
4082 TGTACA
TfiI 5539 GAATC G^A[AT]TC
5870 GAATC
XmnI 4825 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5853 GAAAATATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI ApoI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BglII
BmyI BpmI BpuAI BsaAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoRV
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI SspI StuI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI