################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## AatII Acc65I AcyI AhdI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BbeI BcnI BlnI BsaBI BsaHI BsiEI BsiWI BsmBI BsrBI BssHII BssSI BstBI CpoI EaeI EagI Eam1105I EclXI Eco52I EcoRI FseI FspI HgaI Hin1I KasI KpnI KspI MamI MluI MluNI MscI NaeI NarI NciI NgoMIV NotI NruI NspV PacI PflFI PmeI PpuMI PshBI PvuI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SwaI Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccII 2945 CGCG CG^CG AccIII 5429 TCCGGA T^CCGGA AclI 6700 AACGTT AA^CGTT AflII 5293 CTTAAG C^TTAAG AocI 426 CCTCAGG CC^T[ACGT]AGG Aor51HI 4308 AGCGCT AGC^GCT ApaI 4774 GGGCCC GGGCC^C BamHI 229 GGATCC G^GATCC BanI 323 GGTGCC G^G[CT][GA]CC BbrPI 4365 CACGTG CAC^GTG BbvCI 2317 CCTCAGC CCTCAGC(-5/-2) BcgI 184 GCACACCACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 3603 TGATCA T^GATCA BfrI 5293 CTTAAG C^TTAAG BglI 3848 GCCTAAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 120 GCTCAGC GC^T[ACGT]AGC Bpu1102I 120 GCTCAGC GC^T[ACGT]AGC BseAI 5429 TCCGGA T^CCGGA BspDI 6523 ATCGAT AT^CGAT BspEI 5429 TCCGGA T^CCGGA Bst1107I 3345 GTATAC GTA^TAC BstUI 2945 CGCG CG^CG BstZ17I 3345 GTATAC GTA^TAC Bsu36I 426 CCTCAGG CC^T[ACGT]AGG CelII 120 GCTCAGC GC^T[ACGT]AGC ClaI 6523 ATCGAT AT^CGAT DrdI 237 GACACCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 4308 AGCGCT AGC^GCT Eco81I 426 CCTCAGG CC^T[ACGT]AGG EcoNI 289 CCTCATACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 949 GATATC GAT^ATC EcoT22I 6396 ATGCAT ATGCA^T FauI 5388 GCGGG CCCGC(4/6) FbaI 3603 TGATCA T^GATCA HaeII 4308 AGCGCT [GA]GCGC^[CT] HpaI 2003 GTTAAC GTT^AAC MroI 5429 TCCGGA T^CCGGA MvnI 2945 CGCG CG^CG NdeI 5717 CATATG CA^TATG NsiI 6396 ATGCAT ATGCA^T PmaCI 4365 CACGTG CAC^GTG PmlI 4365 CACGTG CAC^GTG PshAI 1808 GACCCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 6700 AACGTT AA^CGTT SapI 1230 GAAGAGC GCTCTTC(1/4) ScaI 3867 AGTACT AGT^ACT ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccI 3345 GTATAC GT^[AC][GT]AC 4380 GTCTAC AgeI 3088 ACCGGT A^CCGGT 5742 ACCGGT Alw44I 3001 GTGCAC G^TGCAC 5572 GTGCAC ApaLI 3001 GTGCAC G^TGCAC 5572 GTGCAC BsaAI 2983 TACGTG [CT]AC^GT[GA] 4365 CACGTG Bsp1407I 4211 TGTACA T^GTACA 6558 TGTACA BspLU11I 3552 ACATGT A^CATGT 5602 ACATGT BsrDI 6595 CATTGC GCAATG(2/0) 7263 GCAATG BsrFI 3088 ACCGGT [GA]^CCGG[CT] 5742 ACCGGT BsrGI 4211 TGTACA T^GTACA 6558 TGTACA CfoI 610 GCGC GCG^C 4309 GCGC Cfr10I 3088 ACCGGT [GA]^CCGG[CT] 5742 ACCGGT DraIII 5357 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG 5413 CACAGGGTG HhaI 610 GCGC GCG^C 4309 GCGC HinP1I 610 GCGC G^CGC 4309 GCGC HincII 2003 GTTAAC GT[CT]^[GA]AC 5515 GTTGAC HindII 2003 GTTAAC GT[CT]^[GA]AC 5515 GTTGAC NcoI 3511 CCATGG C^CATGG 4779 CCATGG PaeR7I 192 CTCGAG C^TCGAG 5399 CTCGAG PinAI 3088 ACCGGT A^CCGGT 5742 ACCGGT SexAI 2821 ACCAGGT A^CC[AT]GGT 6663 ACCAGGT SspBI 4211 TGTACA T^GTACA 6558 TGTACA StuI 1528 AGGCCT AGG^CCT 7676 AGGCCT XhoI 192 CTCGAG C^TCGAG 5399 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AspHI AvaI AvaII BanII BbsI BbvI BciVI BfaI BglII BmyI BpmI BpuAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspHI BspMI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraI DraII DsaI EarI EcoO109I EcoO65I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MfeI MflI MnlI MseI MslI MspA1I MspI MunI MvaI MwoI NdeII NheI NlaIII NlaIV NspI PflMI PleI PstI PvuII RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI