##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AcyI AhdI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BalI BbeI BcnI
BlnI BsaBI BsaHI BsiEI BsiWI
BsmBI BsrBI BssHII BssSI BstBI
CpoI EaeI EagI Eam1105I EclXI
Eco52I EcoRI FseI FspI HgaI
Hin1I KasI KpnI KspI MamI
MluI MluNI MscI NaeI NarI
NciI NgoMIV NotI NruI NspV
PacI PflFI PmeI PpuMI PshBI
PvuI RsrII SacII SalI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SwaI
Tth111I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccII 2945 CGCG CG^CG
AccIII 5429 TCCGGA T^CCGGA
AclI 6700 AACGTT AA^CGTT
AflII 5293 CTTAAG C^TTAAG
AocI 426 CCTCAGG CC^T[ACGT]AGG
Aor51HI 4308 AGCGCT AGC^GCT
ApaI 4774 GGGCCC GGGCC^C
BamHI 229 GGATCC G^GATCC
BanI 323 GGTGCC G^G[CT][GA]CC
BbrPI 4365 CACGTG CAC^GTG
BbvCI 2317 CCTCAGC CCTCAGC(-5/-2)
BcgI 184 GCACACCACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3603 TGATCA T^GATCA
BfrI 5293 CTTAAG C^TTAAG
BglI 3848 GCCTAAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 120 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 120 GCTCAGC GC^T[ACGT]AGC
BseAI 5429 TCCGGA T^CCGGA
BspDI 6523 ATCGAT AT^CGAT
BspEI 5429 TCCGGA T^CCGGA
Bst1107I 3345 GTATAC GTA^TAC
BstUI 2945 CGCG CG^CG
BstZ17I 3345 GTATAC GTA^TAC
Bsu36I 426 CCTCAGG CC^T[ACGT]AGG
CelII 120 GCTCAGC GC^T[ACGT]AGC
ClaI 6523 ATCGAT AT^CGAT
DrdI 237 GACACCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4308 AGCGCT AGC^GCT
Eco81I 426 CCTCAGG CC^T[ACGT]AGG
EcoNI 289 CCTCATACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 949 GATATC GAT^ATC
EcoT22I 6396 ATGCAT ATGCA^T
FauI 5388 GCGGG CCCGC(4/6)
FbaI 3603 TGATCA T^GATCA
HaeII 4308 AGCGCT [GA]GCGC^[CT]
HpaI 2003 GTTAAC GTT^AAC
MroI 5429 TCCGGA T^CCGGA
MvnI 2945 CGCG CG^CG
NdeI 5717 CATATG CA^TATG
NsiI 6396 ATGCAT ATGCA^T
PmaCI 4365 CACGTG CAC^GTG
PmlI 4365 CACGTG CAC^GTG
PshAI 1808 GACCCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 6700 AACGTT AA^CGTT
SapI 1230 GAAGAGC GCTCTTC(1/4)
ScaI 3867 AGTACT AGT^ACT
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 3345 GTATAC GT^[AC][GT]AC
4380 GTCTAC
AgeI 3088 ACCGGT A^CCGGT
5742 ACCGGT
Alw44I 3001 GTGCAC G^TGCAC
5572 GTGCAC
ApaLI 3001 GTGCAC G^TGCAC
5572 GTGCAC
BsaAI 2983 TACGTG [CT]AC^GT[GA]
4365 CACGTG
Bsp1407I 4211 TGTACA T^GTACA
6558 TGTACA
BspLU11I 3552 ACATGT A^CATGT
5602 ACATGT
BsrDI 6595 CATTGC GCAATG(2/0)
7263 GCAATG
BsrFI 3088 ACCGGT [GA]^CCGG[CT]
5742 ACCGGT
BsrGI 4211 TGTACA T^GTACA
6558 TGTACA
CfoI 610 GCGC GCG^C
4309 GCGC
Cfr10I 3088 ACCGGT [GA]^CCGG[CT]
5742 ACCGGT
DraIII 5357 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
5413 CACAGGGTG
HhaI 610 GCGC GCG^C
4309 GCGC
HinP1I 610 GCGC G^CGC
4309 GCGC
HincII 2003 GTTAAC GT[CT]^[GA]AC
5515 GTTGAC
HindII 2003 GTTAAC GT[CT]^[GA]AC
5515 GTTGAC
NcoI 3511 CCATGG C^CATGG
4779 CCATGG
PaeR7I 192 CTCGAG C^TCGAG
5399 CTCGAG
PinAI 3088 ACCGGT A^CCGGT
5742 ACCGGT
SexAI 2821 ACCAGGT A^CC[AT]GGT
6663 ACCAGGT
SspBI 4211 TGTACA T^GTACA
6558 TGTACA
StuI 1528 AGGCCT AGG^CCT
7676 AGGCCT
XhoI 192 CTCGAG C^TCGAG
5399 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BanII BbsI BbvI
BciVI BfaI BglII BmyI BpmI
BpuAI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspHI
BspMI BsrI BssKI BstAPI BstEII
BstF5I BstNI BstPI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DraII DsaI EarI EcoO109I EcoO65I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MfeI MflI MnlI
MseI MslI MspA1I MspI MunI
MvaI MwoI NdeII NheI NlaIII
NlaIV NspI PflMI PleI PstI
PvuII RcaI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmnI