##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII AccII AccIII AclI AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AspEI AviII AvrII
BamHI BbrPI BcgI BlnI BsaAI
BsaWI BseAI BsiWI Bsp1407I BspEI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstEII BstPI BstUI
BstZ17I Cfr10I CpoI DraII DrdI
Eam1105I Eco47III EcoO109I EcoO65I FseI
FspI KspI MluI MroI MvnI
NaeI NgoMIV NotI NruI PacI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI RsrII SacI
SacII SbfI SfiI SgrAI SmaI
SnaBI Sse8387I SspBI SwaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 5893 GGTACC G^GTACC
AcyI 7791 GGCGCC G[GA]^CG[CT]C
AseI 7323 ATTAAT AT^TAAT
AsnI 7323 ATTAAT AT^TAAT
Asp718I 5893 GGTACC G^GTACC
AspHI 4854 GAGCAC G[AT]GC[AT]^C
AspI 3266 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 7791 GGCGCC GGCGC^C
BglI 2580 GCCCGGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 804 AGATCT A^GATCT
BsaHI 7791 GGCGCC G[GA]^CG[CT]C
BsiEI 39 CGGCCG CG[GA][CT]^CG
BsiHKAI 4854 GAGCAC G[AT]GC[AT]^C
BspDI 2056 ATCGAT AT^CGAT
BstAPI 2511 GCAGACCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1432 TTCGAA TT^CGAA
CfoI 7792 GCGC GCG^C
ClaI 2056 ATCGAT AT^CGAT
DraIII 1417 CACGTCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 39 CGGCCG C^GGCCG
EclXI 39 CGGCCG C^GGCCG
Eco52I 39 CGGCCG C^GGCCG
EcoT22I 1704 ATGCAT ATGCA^T
HaeII 7791 GGCGCC [GA]GCGC^[CT]
HhaI 7792 GCGC GCG^C
Hin1I 7791 GGCGCC G[GA]^CG[CT]C
HinP1I 7792 GCGC G^CGC
KasI 7791 GGCGCC G^GCGCC
KpnI 5893 GGTACC GGTAC^C
NarI 7791 GGCGCC GG^CGCC
NheI 5685 GCTAGC G^CTAGC
NsiI 1704 ATGCAT ATGCA^T
NspV 1432 TTCGAA TT^CGAA
PaeR7I 1130 CTCGAG C^TCGAG
PflFI 3266 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3864 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 7323 ATTAAT AT^TAAT
SalI 1453 GTCGAC G^TCGAC
SapI 4121 GAAGAGC GCTCTTC(1/4)
SfoI 7791 GGCGCC GGC^GCC
SfuI 1432 TTCGAA TT^CGAA
Tth111I 3266 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3864 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1090 CCATCAAAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1130 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflII 2255 CTTAAG C^TTAAG
4230 CTTAAG
AocI 5694 CCTCAGG CC^T[ACGT]AGG
6021 CCTAAGG
AvaI 117 CTCGGG C^[CT]CG[GA]G
1130 CTCGAG
BalI 2194 TGGCCA TGG^CCA
6419 TGGCCA
BanII 174 GAGCCC G[GA]GC[CT]^C
997 GAGCCC
BclI 647 TGATCA T^GATCA
4868 TGATCA
BfrI 2255 CTTAAG C^TTAAG
4230 CTTAAG
BlpI 171 GCTGAGC GC^T[ACGT]AGC
7446 GCTGAGC
Bpu1102I 171 GCTGAGC GC^T[ACGT]AGC
7446 GCTGAGC
BsaBI 2049 GATTGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4542 GATGACTATC
BsoBI 117 CTCGGG C^[CT]CG[GA]G
1130 CTCGAG
Bsu36I 5694 CCTCAGG CC^T[ACGT]AGG
6021 CCTAAGG
CelII 171 GCTGAGC GC^T[ACGT]AGC
7446 GCTGAGC
Eco81I 5694 CCTCAGG CC^T[ACGT]AGG
6021 CCTAAGG
FbaI 647 TGATCA T^GATCA
4868 TGATCA
HpaI 5498 GTTAAC GTT^AAC
7512 GTTAAC
MamI 2049 GATTGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4542 GATGACTATC
MluNI 2194 TGGCCA TGG^CCA
6419 TGGCCA
MscI 2194 TGGCCA TGG^CCA
6419 TGGCCA
NdeI 712 CATATG CA^TATG
4943 CATATG
SexAI 2440 ACCTGGT A^CC[AT]GGT
5357 ACCTGGT
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AvaII
BanI BbsI BbvCI BbvI BciVI
BcnI BfaI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
Bsp1286I BspHI BspMI BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DsaI EaeI EarI EcoNI EcoRI
EcoRII EcoRV EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MfeI MflI MnlI
MseI MslI MspA1I MspI MunI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SpeI SphI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XhoII
XmnI