##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AhdI AocI AscI Asp718I
AspEI BalI BamHI BbeI BbvCI
BcgI BfrI BglI BglII BlpI
Bpu1102I BsaBI BseAI BsiWI BsmBI
BsmI BspDI BspEI BsrDI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRV FseI HindIII KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MunI NarI
NheI NotI NruI NspV PacI
PmeI PshAI Psp1406I PvuI RsrII
SacII SalI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI StuI
SwaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AcyI 215 GGCGTC G[GA]^CG[CT]C
AgeI 591 ACCGGT A^CCGGT
Alw44I 2321 GTGCAC G^TGCAC
AlwI 301 GATCC GGATC(4/5)
AlwNI 1176 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 2093 GGGCCC GGGCC^C
ApaLI 2321 GTGCAC G^TGCAC
AspI 803 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 28 TGCGCA TGC^GCA
AvrII 64 CCTAGG C^CTAGG
BbsI 878 GTCTTC GAAGAC(2/6)
BclI 448 TGATCA T^GATCA
BlnI 64 CCTAGG C^CTAGG
BpuAI 878 GTCTTC GAAGAC(2/6)
BsaHI 215 GGCGTC G[GA]^CG[CT]C
BsaI 540 GAGACC GGTCTC(1/5)
BseRI 1407 GAGGAG GAGGAG(10/8)
BsgI 1113 GTGCAG GTGCAG(16/14)
BsiEI 910 CGGTCG CG[GA][CT]^CG
Bsp1407I 2022 TGTACA T^GTACA
BspHI 1064 TCATGA T^CATGA
BspMI 1705 ACCTGC ACCTGC(4/8)
BsrBI 236 GAGCGG CCGCTC(-3/-3)
BsrGI 2022 TGTACA T^GTACA
BstYI 300 AGATCC [GA]^GATC[CT]
DraI 2384 TTTAAA TTT^AAA
EcoRI 2203 GAATTC G^AATTC
EcoT22I 375 ATGCAT ATGCA^T
FbaI 448 TGATCA T^GATCA
FspI 28 TGCGCA TGC^GCA
Hin1I 215 GGCGTC G[GA]^CG[CT]C
HincII 891 GTTAAC GT[CT]^[GA]AC
HindII 891 GTTAAC GT[CT]^[GA]AC
HpaI 891 GTTAAC GTT^AAC
MflI 300 AGATCC [GA]^GATC[CT]
MslI 607 CACTTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NsiI 375 ATGCAT ATGCA^T
PaeR7I 233 CTCGAG C^TCGAG
PflFI 803 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1175 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 591 ACCGGT A^CCGGT
PvuII 32 CAGCTG CAG^CTG
RcaI 1064 TCATGA T^CATGA
SacI 230 GAGCTC GAGCT^C
SapI 2058 GAAGAGC GCTCTTC(1/4)
SbfI 151 CCTGCAGG CCTGCA^GG
SmaI 1195 CCCGGG CCC^GGG
SpeI 1595 ACTAGT A^CTAGT
SphI 146 GCATGC GCATG^C
Sse8387I 151 CCTGCAGG CCTGCA^GG
SspBI 2022 TGTACA T^GTACA
SspI 1882 AATATT AAT^ATT
TfiI 646 GAATC G^A[AT]TC
Tth111I 803 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1175 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1534 TCTAGA T^CTAGA
XhoI 233 CTCGAG C^TCGAG
XhoII 300 AGATCC [GA]^GATC[CT]
XmaCI 1195 CCCGGG C^CCGGG
XmaI 1195 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Aor51HI 200 AGCGCT AGC^GCT
660 AGCGCT
AseI 1464 ATTAAT AT^TAAT
1885 ATTAAT
AsnI 1464 ATTAAT AT^TAAT
1885 ATTAAT
BbrPI 76 CACGTG CAC^GTG
311 CACGTG
BciVI 493 GGATAC GTATCC(6/5)
1591 GGATAC
BcnI 1195 CCCGG CC^[GC]GG
1196 CCGGG
BpmI 942 CTGGAG CTGGAG(16/14)
2225 CTGGAG
BsaAI 76 CACGTG [CT]AC^GT[GA]
311 CACGTG
BsaWI 303 TCCGGT [AT]^CCGG[AT]
591 ACCGGT
BspLU11I 1203 ACATGT A^CATGT
1684 ACATGT
Eco47III 200 AGCGCT AGC^GCT
660 AGCGCT
HgaI 69 GACGC GACGC(5/10)
216 GCGTC
HphI 2055 GGTGA GGTGA(8/7)
1208 TCACC
NaeI 43 GCCGGC GCC^GGC
585 GCCGGC
NciI 1195 CCCGG CC^[GC]GG
1196 CCGGG
NcoI 268 CCATGG C^CATGG
791 CCATGG
NdeI 1954 CATATG CA^TATG
1983 CATATG
NgoMIV 43 GCCGGC G^CCGGC
585 GCCGGC
PmaCI 76 CACGTG CAC^GTG
311 CACGTG
PmlI 76 CACGTG CAC^GTG
311 CACGTG
PshBI 1464 ATTAAT AT^TAAT
1885 ATTAAT
PstI 152 CTGCAG CTGCA^G
2064 CTGCAG
SmlI 233 CTCGAG C^T[CT][GA]AG
1853 CTCAAG
XcmI 1946 CCAAAAGGCATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2278 CCACCTCACTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI ApoI Asp700I AspHI AvaI
AvaII BanI BanII BbvI BfaI
BmyI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
NspI PleI PpuMI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XmnI