##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AccIII AclI AgeI AscI
AviII AvrII BbeI BclI BlnI
BsaWI BseAI BsiEI BsiWI BspDI
BspEI BstBI ClaI CpoI DrdI
EagI EclXI Eco52I EcoRI FbaI
FseI FspI HindIII KasI KspI
MluI MroI NaeI NarI NgoMIV
NheI NotI NspV PacI PaeR7I
PinAI PmeI PshAI Psp1406I PstI
PvuI RsrII SacII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SpeI Sse8387I StuI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 6818 GGTACC G^GTACC
AcyI 477 GGCGTC G[GA]^CG[CT]C
AhdI 955 GACTGTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 5954 CCTCAGG CC^T[ACGT]AGG
Aor51HI 4268 AGCGCT AGC^GCT
ApaI 7405 GGGCCC GGGCC^C
Asp718I 6818 GGTACC G^GTACC
AspEI 955 GACTGTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 792 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 5846 CCCGAG C^[CT]CG[GA]G
BamHI 6160 GGATCC G^GATCC
BbrPI 3766 CACGTG CAC^GTG
BsaBI 6432 GATATTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 477 GGCGTC G[GA]^CG[CT]C
BsoBI 5846 CCCGAG C^[CT]CG[GA]G
BspLU11I 2936 ACATGT A^CATGT
BsrBI 821 CCGCTC CCGCTC(-3/-3)
BsrFI 2285 GCCGGT [GA]^CCGG[CT]
BssHII 5781 GCGCGC G^CGCGC
Bsu36I 5954 CCTCAGG CC^T[ACGT]AGG
Cfr10I 2285 GCCGGT [GA]^CCGG[CT]
Eam1105I 955 GACTGTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4268 AGCGCT AGC^GCT
Eco81I 5954 CCTCAGG CC^T[ACGT]AGG
EcoRV 6756 GATATC GAT^ATC
EcoT22I 4374 ATGCAT ATGCA^T
Hin1I 477 GGCGTC G[GA]^CG[CT]C
KpnI 6818 GGTACC GGTAC^C
MamI 6432 GATATTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 5453 CAATTG C^AATTG
MunI 5453 CAATTG C^AATTG
NruI 1836 TCGCGA TCG^CGA
NsiI 4374 ATGCAT ATGCA^T
PflFI 792 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3766 CACGTG CAC^GTG
PmlI 3766 CACGTG CAC^GTG
SexAI 43 ACCAGGT A^CC[AT]GGT
SnaBI 1065 TACGTA TAC^GTA
Tth111I 792 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 3531 GTATAC GT^[AC][GT]AC
3784 GTATAC
BbvCI 161 CCTCAGC CCTCAGC(-5/-2)
3370 GCTGAGG
BcgI 1839 CGATGGGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5826 CGATTCTCCTGC
BcnI 1093 CCCGG CC^[GC]GG
5815 CCGGG
BglII 3349 AGATCT A^GATCT
7283 AGATCT
BlpI 894 GCTGAGC GC^T[ACGT]AGC
1630 GCTGAGC
Bpu1102I 894 GCTGAGC GC^T[ACGT]AGC
1630 GCTGAGC
BsaAI 1065 TACGTA [CT]AC^GT[GA]
3766 CACGTG
BsmBI 4252 CGTCTC CGTCTC(1/5)
465 GAGACG
BspHI 1743 TCATGA T^CATGA
2347 TCATGA
BssSI 705 CACGAG CACGAG(-5/-1)
5040 CACGAG
Bst1107I 3531 GTATAC GTA^TAC
3784 GTATAC
BstZ17I 3531 GTATAC GTA^TAC
3784 GTATAC
CelII 894 GCTGAGC GC^T[ACGT]AGC
1630 GCTGAGC
EcoNI 1582 CCTTGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1751 CCTTCTGGAGG
HaeII 720 GGCGCT [GA]GCGC^[CT]
4268 AGCGCT
HpaI 4483 GTTAAC GTT^AAC
7149 GTTAAC
NciI 1093 CCCGG CC^[GC]GG
5815 CCGGG
PflMI 1108 CCATCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2464 CCAGGTGCTGG
RcaI 1743 TCATGA T^CATGA
2347 TCATGA
SacI 1122 GAGCTC GAGCT^C
4873 GAGCTC
SphI 4376 GCATGC GCATG^C
7428 GCATGC
SwaI 2415 ATTTAAAT ATTT^AAAT
3833 ATTTAAAT
Van91I 1108 CCATCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2464 CCAGGTGCTGG
XbaI 3346 TCTAGA T^CTAGA
7599 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AciI AcsI AfaI AflII
AflIII AluI Alw44I AlwI AlwNI
ApaLI ApoI AseI AsnI Asp700I
AspHI AvaII BalI BanI BanII
BbsI BbvI BciVI BfaI BfrI
BglI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
Bsp1407I BspMI BsrDI BsrGI BsrI
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI EarI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NcoI NdeI NdeII NlaIII NlaIV
NspI PleI PpuMI PshBI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmnI