################################## # NO CUT ENZYMES ( 62 enzymes) # ################################## AatII AccIII AclI AgeI AscI AviII AvrII BbeI BclI BlnI BsaWI BseAI BsiEI BsiWI BspDI BspEI BstBI ClaI CpoI DrdI EagI EclXI Eco52I EcoRI FbaI FseI FspI HindIII KasI KspI MluI MroI NaeI NarI NgoMIV NheI NotI NspV PacI PaeR7I PinAI PmeI PshAI Psp1406I PstI PvuI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SpeI Sse8387I StuI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### Acc65I 6818 GGTACC G^GTACC AcyI 477 GGCGTC G[GA]^CG[CT]C AhdI 955 GACTGTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 5954 CCTCAGG CC^T[ACGT]AGG Aor51HI 4268 AGCGCT AGC^GCT ApaI 7405 GGGCCC GGGCC^C Asp718I 6818 GGTACC G^GTACC AspEI 955 GACTGTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 792 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 5846 CCCGAG C^[CT]CG[GA]G BamHI 6160 GGATCC G^GATCC BbrPI 3766 CACGTG CAC^GTG BsaBI 6432 GATATTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 477 GGCGTC G[GA]^CG[CT]C BsoBI 5846 CCCGAG C^[CT]CG[GA]G BspLU11I 2936 ACATGT A^CATGT BsrBI 821 CCGCTC CCGCTC(-3/-3) BsrFI 2285 GCCGGT [GA]^CCGG[CT] BssHII 5781 GCGCGC G^CGCGC Bsu36I 5954 CCTCAGG CC^T[ACGT]AGG Cfr10I 2285 GCCGGT [GA]^CCGG[CT] Eam1105I 955 GACTGTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 4268 AGCGCT AGC^GCT Eco81I 5954 CCTCAGG CC^T[ACGT]AGG EcoRV 6756 GATATC GAT^ATC EcoT22I 4374 ATGCAT ATGCA^T Hin1I 477 GGCGTC G[GA]^CG[CT]C KpnI 6818 GGTACC GGTAC^C MamI 6432 GATATTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 5453 CAATTG C^AATTG MunI 5453 CAATTG C^AATTG NruI 1836 TCGCGA TCG^CGA NsiI 4374 ATGCAT ATGCA^T PflFI 792 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 3766 CACGTG CAC^GTG PmlI 3766 CACGTG CAC^GTG SexAI 43 ACCAGGT A^CC[AT]GGT SnaBI 1065 TACGTA TAC^GTA Tth111I 792 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccI 3531 GTATAC GT^[AC][GT]AC 3784 GTATAC BbvCI 161 CCTCAGC CCTCAGC(-5/-2) 3370 GCTGAGG BcgI 1839 CGATGGGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 5826 CGATTCTCCTGC BcnI 1093 CCCGG CC^[GC]GG 5815 CCGGG BglII 3349 AGATCT A^GATCT 7283 AGATCT BlpI 894 GCTGAGC GC^T[ACGT]AGC 1630 GCTGAGC Bpu1102I 894 GCTGAGC GC^T[ACGT]AGC 1630 GCTGAGC BsaAI 1065 TACGTA [CT]AC^GT[GA] 3766 CACGTG BsmBI 4252 CGTCTC CGTCTC(1/5) 465 GAGACG BspHI 1743 TCATGA T^CATGA 2347 TCATGA BssSI 705 CACGAG CACGAG(-5/-1) 5040 CACGAG Bst1107I 3531 GTATAC GTA^TAC 3784 GTATAC BstZ17I 3531 GTATAC GTA^TAC 3784 GTATAC CelII 894 GCTGAGC GC^T[ACGT]AGC 1630 GCTGAGC EcoNI 1582 CCTTGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1751 CCTTCTGGAGG HaeII 720 GGCGCT [GA]GCGC^[CT] 4268 AGCGCT HpaI 4483 GTTAAC GTT^AAC 7149 GTTAAC NciI 1093 CCCGG CC^[GC]GG 5815 CCGGG PflMI 1108 CCATCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2464 CCAGGTGCTGG RcaI 1743 TCATGA T^CATGA 2347 TCATGA SacI 1122 GAGCTC GAGCT^C 4873 GAGCTC SphI 4376 GCATGC GCATG^C 7428 GCATGC SwaI 2415 ATTTAAAT ATTT^AAAT 3833 ATTTAAAT Van91I 1108 CCATCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2464 CCAGGTGCTGG XbaI 3346 TCTAGA T^CTAGA 7599 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 132 enzymes) # ######################################### AccII AciI AcsI AfaI AflII AflIII AluI Alw44I AlwI AlwNI ApaLI ApoI AseI AsnI Asp700I AspHI AvaII BalI BanI BanII BbsI BbvI BciVI BfaI BfrI BglI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BspMI BsrDI BsrGI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NcoI NdeI NdeII NlaIII NlaIV NspI PleI PpuMI PshBI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI