##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
Alw44I ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspHI AspI
AviII AvrII BamHI BbrPI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmI Bsp1407I BspDI BspEI
BsrGI BssHII Bst1107I BstEII BstPI
BstXI BstZ17I CelII ClaI CpoI
DrdI EagI EclXI Eco52I EcoNI
EcoO65I EcoRV FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MamI MfeI MluI MroI
MunI NcoI NdeI NheI NotI
NruI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI ScaI SexAI SgrAI SmaI
SnaBI SpeI SspBI SwaI Tth111I
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 1740 GACGTC GACGT^C
AccI 1076 GTCTAC GT^[AC][GT]AC
AccII 1081 CGCG CG^CG
AfaI 1711 GTAC GT^AC
AflIII 2256 ACATGT A^C[GA][CT]GT
AhdI 1856 GACGGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 48 AGCGCT AGC^GCT
AspEI 1856 GACGGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1165 CTCGGG C^[CT]CG[GA]G
BcgI 36 CGAGAGGCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 821 GTATCC GTATCC(6/5)
BsaAI 1246 TACGTG [CT]AC^GT[GA]
BsoBI 1165 CTCGGG C^[CT]CG[GA]G
BspHI 2391 TCATGA T^CATGA
BspLU11I 2256 ACATGT A^CATGT
BsrBI 57 GAGCGG CCGCTC(-3/-3)
BstAPI 1010 GCAATTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 485 TTCGAA TT^CGAA
BstUI 1081 CGCG CG^CG
DraI 2431 TTTAAA TTT^AAA
DraIII 1662 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1856 GACGGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 756 GAAGAG CTCTTC(1/4)
Eco47III 48 AGCGCT AGC^GCT
EcoRI 1607 GAATTC G^AATTC
EcoT22I 2364 ATGCAT ATGCA^T
HphI 1744 TCACC GGTGA(8/7)
Ksp632I 756 GAAGAG CTCTTC(1/4)
MvnI 1081 CGCG CG^CG
NsiI 2364 ATGCAT ATGCA^T
NspV 485 TTCGAA TT^CGAA
PpuMI 1225 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 1227 GACCTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2391 TCATGA T^CATGA
RsaI 1711 GTAC GT^AC
SbfI 664 CCTGCAGG CCTGCA^GG
SfiI 1191 GGCCTCCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 485 TTCGAA TT^CGAA
SmlI 765 CTCAAG C^T[CT][GA]AG
SphI 42 GCATGC GCATG^C
Sse8387I 664 CCTGCAGG CCTGCA^GG
StuI 508 AGGCCT AGG^CCT
XcmI 1186 CCAGGGGCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
BalI 244 TGGCCA TGG^CCA
594 TGGCCA
BbeI 77 GGCGCC GGCGC^C
1129 GGCGCC
BbvCI 894 CCTCAGC CCTCAGC(-5/-2)
51 GCTGAGG
BcnI 557 CCGGG CC^[GC]GG
1897 CCCGG
BglI 1183 GCCCCAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1192 GCCTCCTGGGC
BpmI 1118 CTCCAG CTGGAG(16/14)
1209 CTGGAG
BsaI 1233 GGTCTC GGTCTC(1/5)
1863 GGTCTC
BseRI 54 GAGGAG GAGGAG(10/8)
237 GAGGAG
BsmBI 1770 CGTCTC CGTCTC(1/5)
2071 CGTCTC
BsrDI 652 CATTGC GCAATG(2/0)
1006 CATTGC
BssSI 632 CACGAG CACGAG(-5/-1)
1681 CTCGTG
BstYI 761 GGATCT [GA]^GATC[CT]
862 AGATCC
DraII 1189 GGGGCCT [GA]G^G[ACGT]CC[CT]
1225 GGGACCT
EcoO109I 1189 GGGGCCT [GA]G^G[ACGT]CC[CT]
1225 GGGACCT
EcoT14I 1053 CCAAGG C^C[AT][AT]GG
1229 CCTTGG
KasI 77 GGCGCC G^GCGCC
1129 GGCGCC
MflI 761 GGATCT [GA]^GATC[CT]
862 AGATCC
MluNI 244 TGGCCA TGG^CCA
594 TGGCCA
MscI 244 TGGCCA TGG^CCA
594 TGGCCA
NaeI 3 GCCGGC GCC^GGC
128 GCCGGC
NarI 77 GGCGCC GG^CGCC
1129 GGCGCC
NciI 557 CCGGG CC^[GC]GG
1897 CCCGG
NgoMIV 3 GCCGGC G^CCGGC
128 GCCGGC
PflMI 1202 CCACGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1692 CCAGCCTGTGG
PstI 665 CTGCAG CTGCA^G
1511 CTGCAG
SfoI 77 GGCGCC GGC^GCC
1129 GGCGCC
SspI 715 AATATT AAT^ATT
776 AATATT
StyI 1053 CCAAGG C^C[AT][AT]GG
1229 CCTTGG
Van91I 1202 CCACGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1692 CCAGCCTGTGG
XhoII 761 GGATCT [GA]^GATC[CT]
862 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcsI AcyI AluI AlwI
AlwNI AocI ApoI AvaII BanI
BanII BbsI BbvI BfaI BmyI
BpuAI BsaHI BsaJI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DsaI EaeI Eco81I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII ItaI MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PvuII Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XbaI