##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AcyI AflII AgeI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp718I AspI AvrII BamHI
BbeI BbrPI BbvCI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaHI
BsiEI BsiWI BsmBI BsmI BspHI
BsrBI BsrFI BssHII BssSI BstEII
BstPI BstXI BstYI CelII Cfr10I
CpoI DraIII EagI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI FseI
HaeII Hin1I KasI KpnI KspI
MfeI MflI MluI MunI NaeI
NarI NgoMIV NheI NotI NruI
PacI PflFI PinAI PmaCI PmeI
PmlI PpuMI PshBI PstI PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI Sse8387I Tth111I XbaI
XcmI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 69 enzymes) #
###################################
AccI 2610 GTATAC GT^[AC][GT]AC
AccII 148 CGCG CG^CG
AccIII 72 TCCGGA T^CCGGA
AclI 917 AACGTT AA^CGTT
AcsI 2010 AAATTT [GA]^AATT[CT]
AflIII 56 ACATGT A^C[GA][CT]GT
AhdI 1771 GACCAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 1522 GATCC GGATC(4/5)
AocI 1373 CCTTAGG CC^T[ACGT]AGG
ApaI 2392 GGGCCC GGGCC^C
ApoI 2010 AAATTT [GA]^AATT[CT]
AspEI 1771 GACCAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 539 CTCGAG C^[CT]CG[GA]G
AviII 684 TGCGCA TGC^GCA
BalI 863 TGGCCA TGG^CCA
BanI 1150 GGCACC G^G[CT][GA]CC
BcgI 2241 GCAAGCCTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1629 GGATAC GTATCC(6/5)
BclI 852 TGATCA T^GATCA
BcnI 21 CCCGG CC^[GC]GG
BsaAI 2387 TACGTG [CT]AC^GT[GA]
BsaBI 2775 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 72 TCCGGA T^CCGGA
BsmFI 19 GTCCC GGGAC(10/14)
BsoBI 539 CTCGAG C^[CT]CG[GA]G
BspDI 2372 ATCGAT AT^CGAT
BspEI 72 TCCGGA T^CCGGA
BspLU11I 56 ACATGT A^CATGT
BspMI 1186 ACCTGC ACCTGC(4/8)
Bst1107I 2610 GTATAC GTA^TAC
BstBI 118 TTCGAA TT^CGAA
BstUI 148 CGCG CG^CG
BstZ17I 2610 GTATAC GTA^TAC
Bsu36I 1373 CCTTAGG CC^T[ACGT]AGG
ClaI 2372 ATCGAT AT^CGAT
DrdI 253 GACAAAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 863 TGGCCA [CT]^GGCC[GA]
Eam1105I 1771 GACCAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1373 CCTTAGG CC^T[ACGT]AGG
EcoT22I 2195 ATGCAT ATGCA^T
FbaI 852 TGATCA T^GATCA
FspI 684 TGCGCA TGC^GCA
HgaI 1823 GCGTC GACGC(5/10)
HincII 1042 GTTAAC GT[CT]^[GA]AC
HindII 1042 GTTAAC GT[CT]^[GA]AC
HpaI 1042 GTTAAC GTT^AAC
MamI 2775 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 863 TGGCCA TGG^CCA
MroI 72 TCCGGA T^CCGGA
MscI 863 TGGCCA TGG^CCA
MslI 1435 CATTTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 148 CGCG CG^CG
NciI 21 CCCGG CC^[GC]GG
NdeI 2305 CATATG CA^TATG
NsiI 2195 ATGCAT ATGCA^T
NspV 118 TTCGAA TT^CGAA
PaeR7I 539 CTCGAG C^TCGAG
PflMI 904 CCATCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 2675 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 917 AACGTT AA^CGTT
SapI 3 GCTCTTC GCTCTTC(1/4)
SfcI 2274 CTATAG C^T[GA][CT]AG
SfuI 118 TTCGAA TT^CGAA
SmlI 539 CTCGAG C^T[CT][GA]AG
SpeI 1290 ACTAGT A^CTAGT
SphI 2220 GCATGC GCATG^C
SwaI 689 ATTTAAAT ATTT^AAAT
Van91I 904 CCATCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 539 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Asp700I 1342 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2079 GAACATTTTC
AvaII 639 GGACC G^G[AT]CC
2674 GGACC
BbsI 161 GTCTTC GAAGAC(2/6)
2682 GTCTTC
BpmI 1712 CTGGAG CTGGAG(16/14)
2482 CTGGAG
BpuAI 161 GTCTTC GAAGAC(2/6)
2682 GTCTTC
BsaI 1319 GAGACC GGTCTC(1/5)
508 GGTCTC
BsgI 1188 CTGCAC GTGCAG(16/14)
1553 CTGCAC
Bsp1407I 1245 TGTACA T^GTACA
2349 TGTACA
BsrGI 1245 TGTACA T^GTACA
2349 TGTACA
CfoI 685 GCGC GCG^C
2224 GCGC
EcoRV 1216 GATATC GAT^ATC
1695 GATATC
FauI 146 CCCGC CCCGC(4/6)
409 GCGGG
HhaI 685 GCGC GCG^C
2224 GCGC
HinP1I 685 GCGC G^CGC
2224 GCGC
NcoI 866 CCATGG C^CATGG
1031 CCATGG
PleI 205 GACTC GAGTC(4/5)
1777 GAGTC
SacI 497 GAGCTC GAGCT^C
2485 GAGCTC
SspBI 1245 TGTACA T^GTACA
2349 TGTACA
XmnI 1342 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2079 GAACATTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
BanII BbvI BfaI BmyI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BsrDI BsrI BssKI
BstAPI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraI DraII DsaI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I