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# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII AcyI AgeI AhdI AscI
AspEI AviII BbeI BbrPI BciVI
BclI BglII BsaAI BsaHI BsiWI
BspHI BsrBI BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Cfr10I CpoI DraII DrdI Eam1105I
EcoO109I EcoO65I FbaI FseI FspI
Hin1I KasI MfeI MluI MunI
NaeI NarI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshAI
PvuI RcaI RsrII SacI SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SnaBI Sse8387I XhoI
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# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 3024 GGTACC G^GTACC
AccI 2920 GTAGAC GT^[AC][GT]AC
AccIII 2688 TCCGGA T^CCGGA
AclI 5439 AACGTT AA^CGTT
Aor51HI 4135 AGCGCT AGC^GCT
ApaI 126 GGGCCC GGGCC^C
Asp718I 3024 GGTACC G^GTACC
AspI 4570 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4520 CCTAGG C^CTAGG
BamHI 4242 GGATCC G^GATCC
BanII 126 GGGCCC G[GA]GC[CT]^C
BcgI 4652 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 165 GCCGAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 4520 CCTAGG C^CTAGG
BlpI 836 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 836 GCTTAGC GC^T[ACGT]AGC
BsaWI 2688 TCCGGA [AT]^CCGG[AT]
BseAI 2688 TCCGGA T^CCGGA
BsiEI 163 CGGCCG CG[GA][CT]^CG
Bsp1407I 1660 TGTACA T^GTACA
BspDI 3338 ATCGAT AT^CGAT
BspEI 2688 TCCGGA T^CCGGA
BsrGI 1660 TGTACA T^GTACA
BstXI 1803 CCATCCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 836 GCTTAGC GC^T[ACGT]AGC
ClaI 3338 ATCGAT AT^CGAT
DraIII 458 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 163 CGGCCG C^GGCCG
EclXI 163 CGGCCG C^GGCCG
Eco47III 4135 AGCGCT AGC^GCT
Eco52I 163 CGGCCG C^GGCCG
EcoRV 6740 GATATC GAT^ATC
HgaI 44 GACGC GACGC(5/10)
HpaI 5883 GTTAAC GTT^AAC
KpnI 3024 GGTACC GGTAC^C
MroI 2688 TCCGGA T^CCGGA
NcoI 2076 CCATGG C^CATGG
NdeI 4187 CATATG CA^TATG
PflFI 4570 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 5439 AACGTT AA^CGTT
SmaI 4640 CCCGGG CCC^GGG
SpeI 3515 ACTAGT A^CTAGT
SspBI 1660 TGTACA T^GTACA
SwaI 4453 ATTTAAAT ATTT^AAAT
Tth111I 4570 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 5188 TCTAGA T^CTAGA
XmaCI 4640 CCCGGG C^CCGGG
XmaI 4640 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AflII 5704 CTTAAG C^TTAAG
7127 CTTAAG
AvaII 664 GGACC G^G[AT]CC
1320 GGACC
BalI 3844 TGGCCA TGG^CCA
5166 TGGCCA
BanI 3024 GGTACC G^G[CT][GA]CC
7313 GGCACC
BbvCI 2049 CCTCAGC CCTCAGC(-5/-2)
4663 CCTCAGC
BfrI 5704 CTTAAG C^TTAAG
7127 CTTAAG
BsgI 3437 GTGCAG GTGCAG(16/14)
6416 GTGCAG
BsmI 2650 GAATGC GAATGC(1/-1)
3312 GCATTC
EcoT22I 2668 ATGCAT ATGCA^T
6363 ATGCAT
HaeII 25 GGCGCT [GA]GCGC^[CT]
4135 AGCGCT
HincII 5789 GTTGAC GT[CT]^[GA]AC
5883 GTTAAC
HindII 5789 GTTGAC GT[CT]^[GA]AC
5883 GTTAAC
KspI 39 CCGCGG CCGC^GG
160 CCGCGG
MluNI 3844 TGGCCA TGG^CCA
5166 TGGCCA
MscI 3844 TGGCCA TGG^CCA
5166 TGGCCA
NsiI 2668 ATGCAT ATGCA^T
6363 ATGCAT
SacII 39 CCGCGG CCGC^GG
160 CCGCGG
SapI 469 GAAGAGC GCTCTTC(1/4)
2740 GAAGAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI AocI
ApaLI ApoI AseI AsnI Asp700I
AspHI AvaI BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaBI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspLU11I BspMI BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraI DsaI EaeI
EarI Eco81I EcoNI EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PshBI PstI PvuII RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI SphI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmnI