##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BamHI BlnI
BpmI BseAI BsiWI BsmBI BsmI
BspDI BspEI BsrBI BsrDI BsrFI
BssHII Bst1107I BstAPI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraIII
Eam1105I Eco47III Eco81I EcoRV EcoT22I
FseI FspI HincII HindII HindIII
HpaI KpnI MfeI MluI MroI
MunI NaeI NdeI NgoMIV NheI
NotI NsiI PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PstI PvuI RsrII SalI SbfI
ScaI SgrAI SnaBI SpeI SphI
Sse8387I StuI SwaI Tth111I XcmI
XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AflII 2244 CTTAAG C^TTAAG
AflIII 2341 ACATGT A^C[GA][CT]GT
BbeI 97 GGCGCC GGCGC^C
BbrPI 508 CACGTG CAC^GTG
BciVI 2143 GGATAC GTATCC(6/5)
BclI 1426 TGATCA T^GATCA
BfrI 2244 CTTAAG C^TTAAG
BglII 1551 AGATCT A^GATCT
BlpI 241 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 241 GCTCAGC GC^T[ACGT]AGC
BsaAI 508 CACGTG [CT]AC^GT[GA]
BsaWI 1209 TCCGGT [AT]^CCGG[AT]
Bsp1407I 1011 TGTACA T^GTACA
BspHI 1500 TCATGA T^CATGA
BspLU11I 2341 ACATGT A^CATGT
BspMI 75 ACCTGC ACCTGC(4/8)
BsrGI 1011 TGTACA T^GTACA
BssSI 1677 CTCGTG CACGAG(-5/-1)
BstBI 929 TTCGAA TT^CGAA
BstXI 190 CCAACTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 241 GCTCAGC GC^T[ACGT]AGC
EcoNI 2400 CCTGGGTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1426 TGATCA T^GATCA
KasI 97 GGCGCC G^GCGCC
KspI 56 CCGCGG CCGC^GG
NarI 97 GGCGCC GG^CGCC
NruI 34 TCGCGA TCG^CGA
NspI 2341 ACATGT [GA]CATG^[CT]
NspV 929 TTCGAA TT^CGAA
PflMI 1355 CCATCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 508 CACGTG CAC^GTG
PmlI 508 CACGTG CAC^GTG
PpuMI 1683 GGGTCCC [GA]G^G[AT]CC[CT]
RcaI 1500 TCATGA T^CATGA
SacI 1190 GAGCTC GAGCT^C
SacII 56 CCGCGG CCGC^GG
SapI 1187 GAAGAGC GCTCTTC(1/4)
SexAI 1443 ACCTGGT A^CC[AT]GGT
SfiI 1087 GGCCAAACGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 97 GGCGCC GGC^GCC
SfuI 929 TTCGAA TT^CGAA
SmaI 110 CCCGGG CCC^GGG
SspBI 1011 TGTACA T^GTACA
SspI 2192 AATATT AAT^ATT
Van91I 1355 CCATCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 70 TCTAGA T^CTAGA
XmaCI 110 CCCGGG C^CCGGG
XmaI 110 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AccI 1213 GTCTAC GT^[AC][GT]AC
2167 GTAGAC
AcyI 97 GGCGCC G[GA]^CG[CT]C
485 GACGCC
AlwI 795 GGATC GGATC(4/5)
1201 GGATC
AlwNI 1319 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
1563 CAGCTTCTG
ApaI 113 GGGCCC GGGCC^C
1095 GGGCCC
Asp700I 1277 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2078 GAAATTGTTC
AvaII 312 GGACC G^G[AT]CC
1684 GGTCC
BalI 939 TGGCCA TGG^CCA
2373 TGGCCA
BanI 97 GGCGCC G^G[CT][GA]CC
1531 GGTGCC
BbvCI 674 GCTGAGG CCTCAGC(-5/-2)
1850 GCTGAGG
BcgI 228 CGATGCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
288 GCACGGTGGTCG
BcnI 110 CCCGG CC^[GC]GG
111 CCGGG
BsaBI 953 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1774 GATCACAATC
BsaHI 97 GGCGCC G[GA]^CG[CT]C
485 GACGCC
BsaI 1163 GAGACC GGTCTC(1/5)
1979 GAGACC
BsiEI 346 CGGCCG CG[GA][CT]^CG
502 CGGCCG
BstEII 697 GGTTACC G^GT[ACGT]ACC
1439 GGTGACC
BstPI 697 GGTTACC G^GT[ACGT]ACC
1439 GGTGACC
BstYI 1201 GGATCT [GA]^GATC[CT]
1551 AGATCT
DrdI 917 GACAATGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
980 GACCAGCGTGTC
EagI 346 CGGCCG C^GGCCG
502 CGGCCG
EclXI 346 CGGCCG C^GGCCG
502 CGGCCG
Eco52I 346 CGGCCG C^GGCCG
502 CGGCCG
EcoO65I 697 GGTTACC G^GT[ACGT]ACC
1439 GGTGACC
EcoRI 1305 GAATTC G^AATTC
1662 GAATTC
FauI 253 GCGGG CCCGC(4/6)
2388 GCGGG
HaeII 52 AGCGCC [GA]GCGC^[CT]
97 GGCGCC
Hin1I 97 GGCGCC G[GA]^CG[CT]C
485 GACGCC
MamI 953 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1774 GATCACAATC
MflI 1201 GGATCT [GA]^GATC[CT]
1551 AGATCT
MluNI 939 TGGCCA TGG^CCA
2373 TGGCCA
MscI 939 TGGCCA TGG^CCA
2373 TGGCCA
NciI 110 CCCGG CC^[GC]GG
111 CCGGG
NcoI 645 CCATGG C^CATGG
1983 CCATGG
PvuII 816 CAGCTG CAG^CTG
1740 CAGCTG
SmlI 1868 CTCAAG C^T[CT][GA]AG
2244 CTTAAG
TspRI 1527 CAGTG CA[GC]TG[ACGT][ACGT]^
2172 CACTG
XhoII 1201 GGATCT [GA]^GATC[CT]
1551 AGATCT
XmnI 1277 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2078 GAAATTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApoI AspHI AvaI BanII BbsI
BbvI BfaI BglI BmyI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TthHB8I