##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AatII AclI AflII AhdI AscI
AseI AsnI AspEI BfrI BsaBI
BsiWI BspDI BspLU11I BsrDI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI Eam1105I EcoO65I EcoRI
FseI HpaI KspI MamI MfeI
MunI NheI NotI NruI NspV
PacI PaeR7I PmeI PshAI PshBI
Psp1406I RsrII SacII SalI SbfI
SexAI SfuI SnaBI SpeI Sse8387I
SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 2909 GTCTAC GT^[AC][GT]AC
AccIII 562 TCCGGA T^CCGGA
AcsI 493 AAATTT [GA]^AATT[CT]
AgeI 2781 ACCGGT A^CCGGT
Aor51HI 2506 AGCGCT AGC^GCT
ApoI 493 AAATTT [GA]^AATT[CT]
Asp700I 3464 GAATCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 3758 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1053 TGCGCA TGC^GCA
BbrPI 2300 CACGTG CAC^GTG
BcgI 7314 GCACAAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1515 GTATCC GTATCC(6/5)
BclI 5863 TGATCA T^GATCA
BsaAI 2300 CACGTG [CT]AC^GT[GA]
BseAI 562 TCCGGA T^CCGGA
Bsp1407I 675 TGTACA T^GTACA
BspEI 562 TCCGGA T^CCGGA
BsrGI 675 TGTACA T^GTACA
BssSI 4207 CTCGTG CACGAG(-5/-1)
Eco47III 2506 AGCGCT AGC^GCT
EcoRV 7038 GATATC GAT^ATC
EcoT22I 6972 ATGCAT ATGCA^T
FbaI 5863 TGATCA T^GATCA
FspI 1053 TGCGCA TGC^GCA
HindIII 6025 AAGCTT A^AGCTT
MluI 2443 ACGCGT A^CGCGT
MroI 562 TCCGGA T^CCGGA
NaeI 67 GCCGGC GCC^GGC
NdeI 1406 CATATG CA^TATG
NgoMIV 67 GCCGGC G^CCGGC
NsiI 6972 ATGCAT ATGCA^T
PflFI 3758 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2781 ACCGGT A^CCGGT
PmaCI 2300 CACGTG CAC^GTG
PmlI 2300 CACGTG CAC^GTG
PvuI 886 CGATCG CGAT^CG
ScaI 447 AGTACT AGT^ACT
SgrAI 2780 CACCGGTG C[GA]^CCGG[CT]G
SphI 6974 GCATGC GCATG^C
SspBI 675 TGTACA T^GTACA
Tth111I 3758 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 3464 GAATCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
Acc65I 1594 GGTACC G^GTACC
6214 GGTACC
Asp718I 1594 GGTACC G^GTACC
6214 GGTACC
BbeI 2541 GGCGCC GGCGC^C
2559 GGCGCC
BsmBI 707 GAGACG CGTCTC(1/5)
725 GAGACG
BsmI 2811 GCATTC GAATGC(1/-1)
7373 GCATTC
BssHII 2270 GCGCGC G^CGCGC
2319 GCGCGC
DrdI 2731 GACCTGCCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4222 GACCCAAGTGTC
HincII 1328 GTTGAC GT[CT]^[GA]AC
5076 GTCAAC
HindII 1328 GTTGAC GT[CT]^[GA]AC
5076 GTCAAC
KasI 2541 GGCGCC G^GCGCC
2559 GGCGCC
KpnI 1594 GGTACC GGTAC^C
6214 GGTACC
NarI 2541 GGCGCC GG^CGCC
2559 GGCGCC
SfoI 2541 GGCGCC GGC^GCC
2559 GGCGCC
TaqI 889 TCGA T^CGA
4042 TCGA
TthHB8I 889 TCGA T^CGA
4042 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 147 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI AspHI AvaI AvaII
AvrII BalI BamHI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BglII BlnI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspHI BspMI BsrBI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EagI EarI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RcaI RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SfiI SmaI SmlI StuI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XcmI XhoII
XmaCI XmaI