################################## # NO CUT ENZYMES ( 54 enzymes) # ################################## AatII AclI AflII AhdI AscI AseI AsnI AspEI BfrI BsaBI BsiWI BspDI BspLU11I BsrDI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI Eam1105I EcoO65I EcoRI FseI HpaI KspI MamI MfeI MunI NheI NotI NruI NspV PacI PaeR7I PmeI PshAI PshBI Psp1406I RsrII SacII SalI SbfI SexAI SfuI SnaBI SpeI Sse8387I SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccI 2909 GTCTAC GT^[AC][GT]AC AccIII 562 TCCGGA T^CCGGA AcsI 493 AAATTT [GA]^AATT[CT] AgeI 2781 ACCGGT A^CCGGT Aor51HI 2506 AGCGCT AGC^GCT ApoI 493 AAATTT [GA]^AATT[CT] Asp700I 3464 GAATCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 3758 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1053 TGCGCA TGC^GCA BbrPI 2300 CACGTG CAC^GTG BcgI 7314 GCACAAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1515 GTATCC GTATCC(6/5) BclI 5863 TGATCA T^GATCA BsaAI 2300 CACGTG [CT]AC^GT[GA] BseAI 562 TCCGGA T^CCGGA Bsp1407I 675 TGTACA T^GTACA BspEI 562 TCCGGA T^CCGGA BsrGI 675 TGTACA T^GTACA BssSI 4207 CTCGTG CACGAG(-5/-1) Eco47III 2506 AGCGCT AGC^GCT EcoRV 7038 GATATC GAT^ATC EcoT22I 6972 ATGCAT ATGCA^T FbaI 5863 TGATCA T^GATCA FspI 1053 TGCGCA TGC^GCA HindIII 6025 AAGCTT A^AGCTT MluI 2443 ACGCGT A^CGCGT MroI 562 TCCGGA T^CCGGA NaeI 67 GCCGGC GCC^GGC NdeI 1406 CATATG CA^TATG NgoMIV 67 GCCGGC G^CCGGC NsiI 6972 ATGCAT ATGCA^T PflFI 3758 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 2781 ACCGGT A^CCGGT PmaCI 2300 CACGTG CAC^GTG PmlI 2300 CACGTG CAC^GTG PvuI 886 CGATCG CGAT^CG ScaI 447 AGTACT AGT^ACT SgrAI 2780 CACCGGTG C[GA]^CCGG[CT]G SphI 6974 GCATGC GCATG^C SspBI 675 TGTACA T^GTACA Tth111I 3758 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 3464 GAATCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### Acc65I 1594 GGTACC G^GTACC 6214 GGTACC Asp718I 1594 GGTACC G^GTACC 6214 GGTACC BbeI 2541 GGCGCC GGCGC^C 2559 GGCGCC BsmBI 707 GAGACG CGTCTC(1/5) 725 GAGACG BsmI 2811 GCATTC GAATGC(1/-1) 7373 GCATTC BssHII 2270 GCGCGC G^CGCGC 2319 GCGCGC DrdI 2731 GACCTGCCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4222 GACCCAAGTGTC HincII 1328 GTTGAC GT[CT]^[GA]AC 5076 GTCAAC HindII 1328 GTTGAC GT[CT]^[GA]AC 5076 GTCAAC KasI 2541 GGCGCC G^GCGCC 2559 GGCGCC KpnI 1594 GGTACC GGTAC^C 6214 GGTACC NarI 2541 GGCGCC GG^CGCC 2559 GGCGCC SfoI 2541 GGCGCC GGC^GCC 2559 GGCGCC TaqI 889 TCGA T^CGA 4042 TCGA TthHB8I 889 TCGA T^CGA 4042 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 147 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI AocI ApaI ApaLI AspHI AvaI AvaII AvrII BalI BamHI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BspMI BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EagI EarI EclXI Eco52I Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RcaI RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SfiI SmaI SmlI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XcmI XhoII XmaCI XmaI