##################################
# NO CUT ENZYMES (  119 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AgeI        Alw44I      AocI        Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp718I     
AspI        AvaI        AviII       BalI        BamHI       
BanI        BbeI        BbrPI       BcgI        BclI        
BfrI        BglII       BlpI        Bpu1102I    BsaBI       
BsaHI       BsaWI       BseAI       BsgI        BsiEI       
BsiWI       BsmBI       BsoBI       Bsp1407I    BspDI       
BspEI       BspMI       BsrBI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstEII      BstPI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DrdI        EaeI        EagI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
FbaI        FseI        FspI        Hin1I       HindIII     
HpaI        KasI        KpnI        KspI        MamI        
MfeI        MluI        MluNI       MroI        MscI        
MunI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshBI       Psp1406I    PvuI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SgrAI       SmaI        
SnaBI       Sse8387I    SspBI       SwaI        Tth111I     
XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccI          673  GTAGAC               GT^[AC][GT]AC
AccII           1  CGCG                 CG^CG
AflIII       1710  ACATGT               A^C[GA][CT]GT
AhdI         1108  GACTTTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI          733  GGGCCC               GGGCC^C
AspEI        1108  GACTTTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         248  GTGCTC               G[AT]GC[AT]^C
AvrII        1905  CCTAGG               C^CTAGG
BciVI          54  GGATAC               GTATCC(6/5)
BcnI          102  CCGGG                CC^[GC]GG
BglI         1531  GCCAATCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         1905  CCTAGG               C^CTAGG
BsaAI         275  CACGTA               [CT]AC^GT[GA]
BsaI         1014  GGTCTC               GGTCTC(1/5)
BsiHKAI       248  GTGCTC               G[AT]GC[AT]^C
BspLU11I     1710  ACATGT               A^CATGT
BsrDI         458  GCAATG               GCAATG(2/0)
BstBI        1328  TTCGAA               TT^CGAA
BstUI           1  CGCG                 CG^CG
BstXI        1428  CCAAGCTGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI         2678  TTTAAA               TTT^AAA
DraII        1776  GGGACCT              [GA]G^G[ACGT]CC[CT]
DraIII       2146  CACTTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         2879  CCATGG               C^C[GA][CT]GG
Eam1105I     1108  GACTTTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I     1776  GGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRI        2687  GAATTC               G^AATTC
EcoT22I      2504  ATGCAT               ATGCA^T
FauI          731  GCGGG                CCCGC(4/6)
HapII         102  CCGG                 C^CGG
HgaI         1617  GCGTC                GACGC(5/10)
HincII       1619  GTCAAC               GT[CT]^[GA]AC
HindII       1619  GTCAAC               GT[CT]^[GA]AC
HpaII         102  CCGG                 C^CGG
MaeII         276  ACGT                 A^CGT
MspI          102  CCGG                 C^CGG
MvnI            1  CGCG                 CG^CG
NciI          102  CCGGG                CC^[GC]GG
NcoI         2879  CCATGG               C^CATGG
NsiI         2504  ATGCAT               ATGCA^T
NspV         1328  TTCGAA               TT^CGAA
PflMI         195  CCATGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        1776  GGGACCT              [GA]G^G[AT]CC[CT]
PshAI        1036  GACCGCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfuI         1328  TTCGAA               TT^CGAA
SpeI         2520  ACTAGT               A^CTAGT
SphI          270  GCATGC               GCATG^C
StuI         1194  AGGCCT               AGG^CCT
Van91I        195  CCATGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         2897  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AlwNI         233  CAGAGACTG            CAG[ACGT][ACGT][ACGT]^CTG
              710  CAGTGACTG           
BanII         733  GGGCCC               G[GA]GC[CT]^C
             2734  GAGCCC              
BbsI          567  GAAGAC               GAAGAC(2/6)
             1402  GAAGAC              
BbvCI        1275  CCTCAGC              CCTCAGC(-5/-2)
              316  GCTGAGG             
BpuAI         567  GAAGAC               GAAGAC(2/6)
             1402  GAAGAC              
BspHI        1478  TCATGA               T^CATGA
             1676  TCATGA              
BstAPI         90  GCAGCTTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2145  GCACTTTGTGC         
BstYI         216  GGATCT               [GA]^GATC[CT]
             1205  GGATCT              
EcoRV         514  GATATC               GAT^ATC
             1291  GATATC              
HaeII         599  GGCGCT               [GA]GCGC^[CT]
             1637  AGCGCC              
MflI          216  GGATCT               [GA]^GATC[CT]
             1205  GGATCT              
NspI          270  GCATGC               [GA]CATG^[CT]
             1710  ACATGT              
PstI          668  CTGCAG               CTGCA^G
             2017  CTGCAG              
PvuII         352  CAGCTG               CAG^CTG
              844  CAGCTG              
RcaI         1478  TCATGA               T^CATGA
             1676  TCATGA              
SspI         2815  AATATT               AAT^ATT
             3018  AATATT              
XhoII         216  GGATCT               [GA]^GATC[CT]
             1205  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   72 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        Asp700I     AvaII       BbvI        BfaI        
BmyI        BpmI        BsaJI       BseRI       BsiYI       
BslI        BsmAI       BsmFI       BsmI        Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       EarI        
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HhaI        HinP1I      HinfI       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MnlI        MseI        MslI        MspA1I      MvaI        
MwoI        NdeII       NlaIII      NlaIV       PleI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XmnI