##################################
# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspI AvaI AviII BalI BamHI
BanI BbeI BbrPI BcgI BclI
BfrI BglII BlpI Bpu1102I BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstEII BstPI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DrdI EaeI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
FbaI FseI FspI Hin1I HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI Sse8387I SspBI SwaI Tth111I
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccI 673 GTAGAC GT^[AC][GT]AC
AccII 1 CGCG CG^CG
AflIII 1710 ACATGT A^C[GA][CT]GT
AhdI 1108 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 733 GGGCCC GGGCC^C
AspEI 1108 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 248 GTGCTC G[AT]GC[AT]^C
AvrII 1905 CCTAGG C^CTAGG
BciVI 54 GGATAC GTATCC(6/5)
BcnI 102 CCGGG CC^[GC]GG
BglI 1531 GCCAATCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1905 CCTAGG C^CTAGG
BsaAI 275 CACGTA [CT]AC^GT[GA]
BsaI 1014 GGTCTC GGTCTC(1/5)
BsiHKAI 248 GTGCTC G[AT]GC[AT]^C
BspLU11I 1710 ACATGT A^CATGT
BsrDI 458 GCAATG GCAATG(2/0)
BstBI 1328 TTCGAA TT^CGAA
BstUI 1 CGCG CG^CG
BstXI 1428 CCAAGCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 2678 TTTAAA TTT^AAA
DraII 1776 GGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 2146 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2879 CCATGG C^C[GA][CT]GG
Eam1105I 1108 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 1776 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 2687 GAATTC G^AATTC
EcoT22I 2504 ATGCAT ATGCA^T
FauI 731 GCGGG CCCGC(4/6)
HapII 102 CCGG C^CGG
HgaI 1617 GCGTC GACGC(5/10)
HincII 1619 GTCAAC GT[CT]^[GA]AC
HindII 1619 GTCAAC GT[CT]^[GA]AC
HpaII 102 CCGG C^CGG
MaeII 276 ACGT A^CGT
MspI 102 CCGG C^CGG
MvnI 1 CGCG CG^CG
NciI 102 CCGGG CC^[GC]GG
NcoI 2879 CCATGG C^CATGG
NsiI 2504 ATGCAT ATGCA^T
NspV 1328 TTCGAA TT^CGAA
PflMI 195 CCATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1776 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 1036 GACCGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfuI 1328 TTCGAA TT^CGAA
SpeI 2520 ACTAGT A^CTAGT
SphI 270 GCATGC GCATG^C
StuI 1194 AGGCCT AGG^CCT
Van91I 195 CCATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2897 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AlwNI 233 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
710 CAGTGACTG
BanII 733 GGGCCC G[GA]GC[CT]^C
2734 GAGCCC
BbsI 567 GAAGAC GAAGAC(2/6)
1402 GAAGAC
BbvCI 1275 CCTCAGC CCTCAGC(-5/-2)
316 GCTGAGG
BpuAI 567 GAAGAC GAAGAC(2/6)
1402 GAAGAC
BspHI 1478 TCATGA T^CATGA
1676 TCATGA
BstAPI 90 GCAGCTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2145 GCACTTTGTGC
BstYI 216 GGATCT [GA]^GATC[CT]
1205 GGATCT
EcoRV 514 GATATC GAT^ATC
1291 GATATC
HaeII 599 GGCGCT [GA]GCGC^[CT]
1637 AGCGCC
MflI 216 GGATCT [GA]^GATC[CT]
1205 GGATCT
NspI 270 GCATGC [GA]CATG^[CT]
1710 ACATGT
PstI 668 CTGCAG CTGCA^G
2017 CTGCAG
PvuII 352 CAGCTG CAG^CTG
844 CAGCTG
RcaI 1478 TCATGA T^CATGA
1676 TCATGA
SspI 2815 AATATT AAT^ATT
3018 AATATT
XhoII 216 GGATCT [GA]^GATC[CT]
1205 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AvaII BbvI BfaI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HhaI HinP1I HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XmnI