##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI Aor51HI AscI
AseI AsnI Asp700I Asp718I AviII
AvrII BbvCI BciVI BclI BfrI
BlnI BsaI BseAI BsmBI BsmI
BspDI BspEI BspHI BspLU11I BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Cfr10I ClaI CpoI
EagI EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HpaI KpnI KspI MfeI
MluI MroI MunI NaeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PpuMI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
SwaI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Alw44I 1201 GTGCAC G^TGCAC
AocI 729 CCTCAGG CC^T[ACGT]AGG
ApaLI 1201 GTGCAC G^TGCAC
AspHI 1201 GTGCAC G[AT]GC[AT]^C
AvaI 258 CTCGGG C^[CT]CG[GA]G
BamHI 373 GGATCC G^GATCC
BanI 46 GGCGCC G^G[CT][GA]CC
BbeI 46 GGCGCC GGCGC^C
BbrPI 1477 CACGTG CAC^GTG
BglI 49 GCCAGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2684 AGATCT A^GATCT
BlpI 1152 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1152 GCTGAGC GC^T[ACGT]AGC
BsaAI 1477 CACGTG [CT]AC^GT[GA]
BsaWI 387 TCCGGT [AT]^CCGG[AT]
BsgI 1548 CTGCAC GTGCAG(16/14)
BsiEI 1268 CGACCG CG[GA][CT]^CG
BsiHKAI 1201 GTGCAC G[AT]GC[AT]^C
BsiWI 215 CGTACG C^GTACG
BsoBI 258 CTCGGG C^[CT]CG[GA]G
BspMI 2832 GCAGGT ACCTGC(4/8)
BstAPI 2563 GCATAATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 363 CCACATGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 729 CCTCAGG CC^T[ACGT]AGG
CelII 1152 GCTGAGC GC^T[ACGT]AGC
DraIII 540 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1269 GACCGAGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 827 GAAGAG CTCTTC(1/4)
Eco81I 729 CCTCAGG CC^T[ACGT]AGG
HgaI 2071 GACGC GACGC(5/10)
KasI 46 GGCGCC G^GCGCC
Ksp632I 827 GAAGAG CTCTTC(1/4)
NarI 46 GGCGCC GG^CGCC
NcoI 1720 CCATGG C^CATGG
NdeI 1583 CATATG CA^TATG
PmaCI 1477 CACGTG CAC^GTG
PmlI 1477 CACGTG CAC^GTG
ScaI 2715 AGTACT AGT^ACT
SfoI 46 GGCGCC GGC^GCC
SmlI 2278 CTCAAG C^T[CT][GA]AG
SspI 118 AATATT AAT^ATT
XbaI 2132 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccII 60 CGCG CG^CG
679 CGCG
AcyI 46 GGCGCC G[GA]^CG[CT]C
2071 GACGCC
AhdI 190 GACGACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
954 GACCAGCAGTC
AspEI 190 GACGACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
954 GACCAGCAGTC
AspI 817 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1269 GACCGAGTC
BalI 144 TGGCCA TGG^CCA
1100 TGGCCA
BbsI 802 GAAGAC GAAGAC(2/6)
1601 GAAGAC
BpuAI 802 GAAGAC GAAGAC(2/6)
1601 GAAGAC
BsaBI 603 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
931 GATCCGTATC
BsaHI 46 GGCGCC G[GA]^CG[CT]C
2071 GACGCC
BsmAI 384 GTCTC GTCTC(1/5)
1751 GTCTC
BsrBI 221 GAGCGG CCGCTC(-3/-3)
1117 GAGCGG
BstUI 60 CGCG CG^CG
679 CGCG
DraII 37 AGGGCCC [GA]G^G[ACGT]CC[CT]
1160 GGGCCCT
EaeI 144 TGGCCA [CT]^GGCC[GA]
1100 TGGCCA
Eam1105I 190 GACGACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
954 GACCAGCAGTC
EcoO109I 37 AGGGCCC [GA]G^G[ACGT]CC[CT]
1160 GGGCCCT
EcoRI 852 GAATTC G^AATTC
1742 GAATTC
Hin1I 46 GGCGCC G[GA]^CG[CT]C
2071 GACGCC
HincII 340 GTCAAC GT[CT]^[GA]AC
2432 GTTGAC
HindII 340 GTCAAC GT[CT]^[GA]AC
2432 GTTGAC
HindIII 1510 AAGCTT A^AGCTT
1950 AAGCTT
MamI 603 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
931 GATCCGTATC
MluNI 144 TGGCCA TGG^CCA
1100 TGGCCA
MscI 144 TGGCCA TGG^CCA
1100 TGGCCA
MvnI 60 CGCG CG^CG
679 CGCG
NspI 558 GCATGT [GA]CATG^[CT]
1310 ACATGC
PflFI 817 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1269 GACCGAGTC
PshAI 2235 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2435 GACCTCGGTC
TaqI 611 TCGA T^CGA
2463 TCGA
Tth111I 817 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1269 GACCGAGTC
TthHB8I 611 TCGA T^CGA
2463 TCGA
XcmI 307 CCACAGCAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
627 CCAGAACATGGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApaI ApoI AvaII BanII
BbvI BcgI BcnI BfaI BmyI
BpmI BsaJI BseRI BsiYI BslI
BsmFI Bsp1286I Bsp1407I BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DsaI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspBI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XhoII