##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
AscI Asp700I AspEI AvrII BbeI
BbrPI BciVI BlnI BseAI BsiEI
BsiWI BspEI BsrFI BssHII Bst1107I
BstBI BstZ17I Cfr10I CpoI EagI
Eam1105I EclXI Eco52I EcoRV FseI
HpaI KasI KspI MroI NaeI
NarI NgoMIV NheI NotI NruI
NspV PaeR7I PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SnaBI SphI
Sse8387I SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 2911 GGTACC G^GTACC
AccI 2533 GTAGAC GT^[AC][GT]AC
AcyI 6933 GGCGTC G[GA]^CG[CT]C
Alw44I 3260 GTGCAC G^TGCAC
ApaLI 3260 GTGCAC G^TGCAC
Asp718I 2911 GGTACC G^GTACC
BamHI 3877 GGATCC G^GATCC
BclI 1457 TGATCA T^GATCA
BsaAI 3323 TACGTG [CT]AC^GT[GA]
BsaBI 3890 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 6933 GGCGTC G[GA]^CG[CT]C
BsaWI 5905 ACCGGA [AT]^CCGG[AT]
BspLU11I 1925 ACATGT A^CATGT
BsrBI 5186 GAGCGG CCGCTC(-3/-3)
BstEII 2521 GGTAACC G^GT[ACGT]ACC
BstPI 2521 GGTAACC G^GT[ACGT]ACC
DraIII 7205 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 529 GACAAACAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2521 GGTAACC G^GT[ACGT]ACC
EcoRI 2694 GAATTC G^AATTC
EcoT22I 3032 ATGCAT ATGCA^T
FbaI 1457 TGATCA T^GATCA
Hin1I 6933 GGCGTC G[GA]^CG[CT]C
HincII 2770 GTCAAC GT[CT]^[GA]AC
HindII 2770 GTCAAC GT[CT]^[GA]AC
HindIII 4784 AAGCTT A^AGCTT
KpnI 2911 GGTACC GGTAC^C
MamI 3890 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 5168 CAATTG C^AATTG
MluI 3734 ACGCGT A^CGCGT
MunI 5168 CAATTG C^AATTG
NsiI 3032 ATGCAT ATGCA^T
PacI 1871 TTAATTAA TTAAT^TAA
ScaI 6260 AGTACT AGT^ACT
SexAI 5819 ACCAGGT A^CC[AT]GGT
SmaI 1429 CCCGGG CCC^GGG
SpeI 3663 ACTAGT A^CTAGT
XmaCI 1429 CCCGGG C^CCGGG
XmaI 1429 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflII 1696 CTTAAG C^TTAAG
1839 CTTAAG
AflIII 1925 ACATGT A^C[GA][CT]GT
3734 ACGCGT
AocI 2735 CCTCAGG CC^T[ACGT]AGG
5646 CCTCAGG
AspI 4489 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5065 GACAGCGTC
AvaI 1397 CTCGGG C^[CT]CG[GA]G
1429 CCCGGG
AviII 572 TGCGCA TGC^GCA
2576 TGCGCA
BcgI 2984 CGAAATGTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5966 CGAAACGATTGC
BfrI 1696 CTTAAG C^TTAAG
1839 CTTAAG
BlpI 160 GCTCAGC GC^T[ACGT]AGC
1627 GCTTAGC
Bpu1102I 160 GCTCAGC GC^T[ACGT]AGC
1627 GCTTAGC
BsoBI 1397 CTCGGG C^[CT]CG[GA]G
1429 CCCGGG
BspDI 434 ATCGAT AT^CGAT
5290 ATCGAT
BspHI 725 TCATGA T^CATGA
2902 TCATGA
BssSI 2468 CACGAG CACGAG(-5/-1)
1024 CTCGTG
Bsu36I 2735 CCTCAGG CC^T[ACGT]AGG
5646 CCTCAGG
CelII 160 GCTCAGC GC^T[ACGT]AGC
1627 GCTTAGC
ClaI 434 ATCGAT AT^CGAT
5290 ATCGAT
Eco81I 2735 CCTCAGG CC^T[ACGT]AGG
5646 CCTCAGG
EcoNI 1106 CCTGTCTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5045 CCTGTGAGAGG
FspI 572 TGCGCA TGC^GCA
2576 TGCGCA
NcoI 740 CCATGG C^CATGG
818 CCATGG
NdeI 2248 CATATG CA^TATG
2689 CATATG
PflFI 4489 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5065 GACAGCGTC
PshAI 1116 GACAAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3428 GACATTTGTC
PvuII 143 CAGCTG CAG^CTG
5203 CAGCTG
RcaI 725 TCATGA T^CATGA
2902 TCATGA
Tth111I 4489 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5065 GACAGCGTC
XbaI 4383 TCTAGA T^CTAGA
4946 TCTAGA
XcmI 1351 CCAAAAATTAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
6862 CCAACGGGTGAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI Aor51HI ApaI ApoI
AseI AsnI AspHI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BglII BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI Bsp1286I Bsp1407I
BspMI BsrDI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI Eco47III EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PshBI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII