##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
AatII AccIII AgeI Aor51HI AscI
AviII BsaBI BseAI BsiWI BsmBI
BspDI BspEI BssSI BstBI BstEII
BstPI ClaI CpoI DrdI Eco47III
EcoO65I EcoRV FseI FspI HpaI
MamI MfeI MluI MroI MunI
NheI NotI NruI NspV PacI
PaeR7I PinAI PmeI PvuI RsrII
SalI SbfI SfiI SfuI SnaBI
Sse8387I SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 3565 GGTACC G^GTACC
AflII 2736 CTTAAG C^TTAAG
AflIII 5232 ACATGT A^C[GA][CT]GT
AhdI 1142 GACGGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 2322 ATTAAT AT^TAAT
AsnI 2322 ATTAAT AT^TAAT
Asp718I 3565 GGTACC G^GTACC
AspEI 1142 GACGGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1424 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 438 TGGCCA TGG^CCA
BamHI 3537 GGATCC G^GATCC
BbvCI 3876 GCTGAGG CCTCAGC(-5/-2)
BclI 5407 TGATCA T^GATCA
BfrI 2736 CTTAAG C^TTAAG
BglII 953 AGATCT A^GATCT
BsaWI 5837 TCCGGT [AT]^CCGG[AT]
BsiEI 392 CGGCCG CG[GA][CT]^CG
Bsp1407I 1580 TGTACA T^GTACA
BspHI 2701 TCATGA T^CATGA
BspLU11I 5232 ACATGT A^CATGT
BsrGI 1580 TGTACA T^GTACA
Bst1107I 7613 GTATAC GTA^TAC
BstZ17I 7613 GTATAC GTA^TAC
EagI 392 CGGCCG C^GGCCG
Eam1105I 1142 GACGGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 392 CGGCCG C^GGCCG
Eco52I 392 CGGCCG C^GGCCG
FbaI 5407 TGATCA T^GATCA
KpnI 3565 GGTACC GGTAC^C
MluNI 438 TGGCCA TGG^CCA
MscI 438 TGGCCA TGG^CCA
NaeI 176 GCCGGC GCC^GGC
NgoMIV 176 GCCGGC G^CCGGC
PflFI 1424 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1321 GACTTACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2322 ATTAAT AT^TAAT
RcaI 2701 TCATGA T^CATGA
ScaI 5935 AGTACT AGT^ACT
SexAI 4871 ACCTGGT A^CC[AT]GGT
SgrAI 175 CGCCGGCG C[GA]^CCGG[CT]G
SpeI 3068 ACTAGT A^CTAGT
SspBI 1580 TGTACA T^GTACA
SspI 7684 AATATT AAT^ATT
StuI 7015 AGGCCT AGG^CCT
Tth111I 1424 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 2513 GTCTAC GT^[AC][GT]AC
7613 GTATAC
AclI 1684 AACGTT AA^CGTT
3698 AACGTT
Asp700I 720 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6660 GAAAACATTC
AvrII 4193 CCTAGG C^CTAGG
6982 CCTAGG
BbrPI 1675 CACGTG CAC^GTG
7100 CACGTG
BcgI 1123 CGAGAACGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1236 GCAGTGGAATCG
BglI 3446 GCCTTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4102 GCCCATTTGGC
BlnI 4193 CCTAGG C^CTAGG
6982 CCTAGG
BsrDI 4571 CATTGC GCAATG(2/0)
5205 GCAATG
BsrFI 176 GCCGGC [GA]^CCGG[CT]
5639 ACCGGC
BstAPI 3925 GCAGCTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4093 GCATGAGTTGC
Cfr10I 176 GCCGGC [GA]^CCGG[CT]
5639 ACCGGC
DraIII 1672 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
1723 CACTAGGTG
EcoNI 357 CCTGAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5294 CCTGGATCAGG
EcoRI 1287 GAATTC G^AATTC
1661 GAATTC
KspI 320 CCGCGG CCGC^GG
4168 CCGCGG
PmaCI 1675 CACGTG CAC^GTG
7100 CACGTG
PmlI 1675 CACGTG CAC^GTG
7100 CACGTG
Psp1406I 1684 AACGTT AA^CGTT
3698 AACGTT
SacII 320 CCGCGG CCGC^GG
4168 CCGCGG
SphI 4680 GCATGC GCATG^C
6495 GCATGC
XcmI 951 CCAGATCTCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
6839 CCAGGAGGCGTTTGG
XmnI 720 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6660 GAAAACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI ApoI AspHI AvaI
AvaII BanI BanII BbeI BbsI
BbvI BciVI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrBI
BsrI BssHII BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NcoI NdeI NdeII NlaIII NlaIV
NsiI NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmaCI
XmaI