##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AscI Asp718I BbrPI BseAI BsiWI
BsmBI BspDI BspEI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI EcoO65I EcoRV FseI KpnI
KspI MfeI MluI MroI MunI
NdeI NheI NotI NruI NspV
PacI PinAI PmaCI PmlI Psp1406I
PvuI RsrII SacII SfuI SgrAI
SnaBI SpeI SspI SwaI
###################################
# ONE CUT ENZYMES ( 25 enzymes) #
###################################
AflII 1606 CTTAAG C^TTAAG
AseI 7328 ATTAAT AT^TAAT
AsnI 7328 ATTAAT AT^TAAT
AvrII 4846 CCTAGG C^CTAGG
BcgI 2017 CGAGGAGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1606 CTTAAG C^TTAAG
BlnI 4846 CCTAGG C^CTAGG
BsaBI 2660 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1840 ACCGGA [AT]^CCGG[AT]
BspLU11I 915 ACATGT A^CATGT
BsrBI 3037 GAGCGG CCGCTC(-3/-3)
DraIII 5102 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
HindIII 3400 AAGCTT A^AGCTT
MamI 2660 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 5423 GCCGGC GCC^GGC
NgoMIV 5423 GCCGGC G^CCGGC
PmeI 7458 GTTTAAAC GTTT^AAAC
PshAI 3793 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 7328 ATTAAT AT^TAAT
SalI 5054 GTCGAC G^TCGAC
ScaI 441 AGTACT AGT^ACT
SfiI 3553 GGCCGTCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 4302 CCCGGG CCC^GGG
XmaCI 4302 CCCGGG C^CCGGG
XmaI 4302 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 2342 GTCTAC GT^[AC][GT]AC
5054 GTCGAC
Alw44I 3330 GTGCAC G^TGCAC
5067 GTGCAC
ApaLI 3330 GTGCAC G^TGCAC
5067 GTGCAC
AviII 873 TGCGCA TGC^GCA
3014 TGCGCA
BamHI 52 GGATCC G^GATCC
4862 GGATCC
BbeI 3200 GGCGCC GGCGC^C
7074 GGCGCC
BbvCI 1589 GCTGAGG CCTCAGC(-5/-2)
5510 GCTGAGG
BciVI 3697 GGATAC GTATCC(6/5)
5848 GGATAC
BclI 3951 TGATCA T^GATCA
6022 TGATCA
BsaAI 3170 TACGTG [CT]AC^GT[GA]
4355 TACGTG
BsiEI 685 CGGCCG CG[GA][CT]^CG
2958 CGGCCG
BssSI 2154 CACGAG CACGAG(-5/-1)
4084 CACGAG
DrdI 3923 GACGTGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6707 GACTTTCCTGTC
EagI 685 CGGCCG C^GGCCG
2958 CGGCCG
EclXI 685 CGGCCG C^GGCCG
2958 CGGCCG
Eco52I 685 CGGCCG C^GGCCG
2958 CGGCCG
FbaI 3951 TGATCA T^GATCA
6022 TGATCA
FspI 873 TGCGCA TGC^GCA
3014 TGCGCA
HpaI 4778 GTTAAC GTT^AAC
6789 GTTAAC
KasI 3200 GGCGCC G^GCGCC
7074 GGCGCC
NarI 3200 GGCGCC GG^CGCC
7074 GGCGCC
SexAI 5103 ACCTGGT A^CC[AT]GGT
6843 ACCAGGT
SfoI 3200 GGCGCC GGC^GCC
7074 GGCGCC
XcmI 3417 CCAAGTTAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3684 CCAGGACTAGCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 160 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AhdI AluI AlwI AlwNI
AocI Aor51HI ApaI ApoI Asp700I
AspEI AspHI AspI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BglI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspHI BspMI
BsrDI BsrFI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI Eam1105I EarI
Eco47III Eco81I EcoNI EcoO109I EcoRI
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NsiI NspI PaeR7I PflFI
PflMI PleI PpuMI PstI PvuII
RcaI RsaI SacI SapI Sau3AI
Sau96I SbfI ScrFI SfaNI SfcI
SmlI SphI Sse8387I SspBI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XbaI XhoI XhoII XmnI