##################################
# NO CUT ENZYMES ( 46 enzymes) #
##################################
AclI AflII AgeI Aor51HI AseI
AsnI AvrII BciVI BfrI BlnI
BspDI BspLU11I Bst1107I BstBI BstZ17I
ClaI CpoI Eco47III EcoRI EcoRV
EcoT22I FseI HpaI KspI MfeI
MluI MunI NheI NotI NruI
NsiI NspV PacI PinAI PmeI
PshBI Psp1406I PvuI RsrII SacII
SfuI SgrAI SpeI SspI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 20 enzymes) #
###################################
Acc65I 4043 GGTACC G^GTACC
AscI 6235 GGCGCGCC GG^CGCGCC
Asp718I 4043 GGTACC G^GTACC
AviII 2792 TGCGCA TGC^GCA
BglII 668 AGATCT A^GATCT
BsaBI 5781 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 677 CGTACG C^GTACG
BsmI 4461 GAATGC GAATGC(1/-1)
BspHI 5492 TCATGA T^CATGA
BsrDI 300 CATTGC GCAATG(2/0)
DraI 6935 TTTAAA TTT^AAA
FspI 2792 TGCGCA TGC^GCA
HindIII 1537 AAGCTT A^AGCTT
KpnI 4043 GGTACC GGTAC^C
MamI 5781 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 511 CATATG CA^TATG
RcaI 5492 TCATGA T^CATGA
SalI 4375 GTCGAC G^TCGAC
ScaI 2003 AGTACT AGT^ACT
SnaBI 6927 TACGTA TAC^GTA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 4375 GTCGAC GT^[AC][GT]AC
5251 GTAGAC
AcsI 1039 AAATTC [GA]^AATT[CT]
1201 GAATTT
AhdI 1453 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2530 GACTCCAAGTC
Alw44I 400 GTGCAC G^TGCAC
5167 GTGCAC
AocI 445 CCTCAGG CC^T[ACGT]AGG
3448 CCTGAGG
ApaLI 400 GTGCAC G^TGCAC
5167 GTGCAC
ApoI 1039 AAATTC [GA]^AATT[CT]
1201 GAATTT
Asp700I 3748 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4962 GAACGACTTC
AspEI 1453 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2530 GACTCCAAGTC
BcgI 2274 CGAAAAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
163 GCAGGCAGCTCG
BclI 2384 TGATCA T^GATCA
5786 TGATCA
Bsu36I 445 CCTCAGG CC^T[ACGT]AGG
3448 CCTGAGG
Eam1105I 1453 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2530 GACTCCAAGTC
Eco81I 445 CCTCAGG CC^T[ACGT]AGG
3448 CCTGAGG
FbaI 2384 TGATCA T^GATCA
5786 TGATCA
PaeR7I 9 CTCGAG C^TCGAG
5650 CTCGAG
SapI 1189 GAAGAGC GCTCTTC(1/4)
2320 GAAGAGC
SbfI 2882 CCTGCAGG CCTGCA^GG
2988 CCTGCAGG
SexAI 4904 ACCTGGT A^CC[AT]GGT
5060 ACCAGGT
SphI 121 GCATGC GCATG^C
2777 GCATGC
Sse8387I 2882 CCTGCAGG CCTGCA^GG
2988 CCTGCAGG
Tsp509I 1040 AATT ^AATT
1202 AATT
XhoI 9 CTCGAG C^TCGAG
5650 CTCGAG
XmnI 3748 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4962 GAACGACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 168 enzymes) #
#########################################
AatII AccII AccIII AciI AcyI
AfaI AflIII AluI AlwI AlwNI
ApaI AspHI AspI AvaI AvaII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I Bsp1407I
BspEI BspMI BsrBI BsrFI BsrGI
BsrI BssHII BssKI BssSI BstAPI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DrdI DsaI EaeI EagI
EarI EclXI Eco52I EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MroI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflFI PflMI PleI
PmaCI PmlI PpuMI PshAI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SfiI SfoI
SmaI SmlI SspBI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII XmaCI XmaI