##################################
# NO CUT ENZYMES ( 41 enzymes) #
##################################
AclI AgeI AscI BciVI Bsp1407I
BspDI BsrGI Bst1107I BstBI BstZ17I
ClaI DraI EcoRV EcoT22I FseI
HindIII HpaI KspI MfeI MunI
NdeI NheI NruI NsiI NspV
PacI PinAI PmeI Psp1406I PvuI
SacII SfuI SgrAI SmaI SnaBI
SspBI SspI SwaI XbaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AatII 5090 GACGTC GACGT^C
Acc65I 1200 GGTACC G^GTACC
AcsI 5966 GAATTC [GA]^AATT[CT]
AflII 6611 CTTAAG C^TTAAG
AocI 6326 CCTGAGG CC^T[ACGT]AGG
ApoI 5966 GAATTC [GA]^AATT[CT]
AseI 7794 ATTAAT AT^TAAT
AsnI 7794 ATTAAT AT^TAAT
Asp718I 1200 GGTACC G^GTACC
AvrII 6988 CCTAGG C^CTAGG
BamHI 5742 GGATCC G^GATCC
BfrI 6611 CTTAAG C^TTAAG
BlnI 6988 CCTAGG C^CTAGG
BsiWI 165 CGTACG C^GTACG
BssHII 1330 GCGCGC G^CGCGC
BstEII 7367 GGTCACC G^GT[ACGT]ACC
BstPI 7367 GGTCACC G^GT[ACGT]ACC
Bsu36I 6326 CCTGAGG CC^T[ACGT]AGG
CpoI 1010 CGGACCG CG^G[AT]CCG
EagI 2164 CGGCCG C^GGCCG
EclXI 2164 CGGCCG C^GGCCG
Eco52I 2164 CGGCCG C^GGCCG
Eco81I 6326 CCTGAGG CC^T[ACGT]AGG
EcoO65I 7367 GGTCACC G^GT[ACGT]ACC
EcoRI 5966 GAATTC G^AATTC
KpnI 1200 GGTACC GGTAC^C
MluI 1470 ACGCGT A^CGCGT
NotI 2163 GCGGCCGC GC^GGCCGC
PshBI 7794 ATTAAT AT^TAAT
RsrII 1010 CGGACCG CG^G[AT]CCG
SbfI 5110 CCTGCAGG CCTGCA^GG
SexAI 444 ACCTGGT A^CC[AT]GGT
SfiI 26 GGCCGCACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 7440 ACTAGT A^CTAGT
Sse8387I 5110 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccIII 1008 TCCGGA T^CCGGA
3588 TCCGGA
AhdI 3194 GACGTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4346 GACAAGAAGTC
AspEI 3194 GACGTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4346 GACAAGAAGTC
AviII 876 TGCGCA TGC^GCA
4068 TGCGCA
BbeI 2435 GGCGCC GGCGC^C
2892 GGCGCC
BbvCI 5316 CCTCAGC CCTCAGC(-5/-2)
7023 GCTGAGG
BcgI 3498 CGATTGTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
90 GCAATGACATCG
BseAI 1008 TCCGGA T^CCGGA
3588 TCCGGA
BspEI 1008 TCCGGA T^CCGGA
3588 TCCGGA
BspLU11I 663 ACATGT A^CATGT
6702 ACATGT
Eam1105I 3194 GACGTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4346 GACAAGAAGTC
FspI 876 TGCGCA TGC^GCA
4068 TGCGCA
KasI 2435 GGCGCC G^GCGCC
2892 GGCGCC
MroI 1008 TCCGGA T^CCGGA
3588 TCCGGA
NarI 2435 GGCGCC GG^CGCC
2892 GGCGCC
PaeR7I 3536 CTCGAG C^TCGAG
6200 CTCGAG
PshAI 859 GACCTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5995 GACCAGTGTC
SacI 959 GAGCTC GAGCT^C
6944 GAGCTC
SalI 4475 GTCGAC G^TCGAC
6206 GTCGAC
SfoI 2435 GGCGCC GGC^GCC
2892 GGCGCC
SphI 2139 GCATGC GCATG^C
5580 GCATGC
XhoI 3536 CTCGAG C^TCGAG
6200 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 160 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
Aor51HI ApaI ApaLI Asp700I AspHI
AspI AvaI AvaII BalI BanI
BanII BbrPI BbsI BbvI BclI
BcnI BfaI BglI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DrdI DsaI EaeI EarI Eco47III
EcoNI EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflFI PflMI PleI
PmaCI PmlI PpuMI PstI PvuII
RcaI RsaI SapI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII XmnI