################################## # NO CUT ENZYMES ( 41 enzymes) # ################################## AclI AgeI AscI BciVI Bsp1407I BspDI BsrGI Bst1107I BstBI BstZ17I ClaI DraI EcoRV EcoT22I FseI HindIII HpaI KspI MfeI MunI NdeI NheI NruI NsiI NspV PacI PinAI PmeI Psp1406I PvuI SacII SfuI SgrAI SmaI SnaBI SspBI SspI SwaI XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AatII 5090 GACGTC GACGT^C Acc65I 1200 GGTACC G^GTACC AcsI 5966 GAATTC [GA]^AATT[CT] AflII 6611 CTTAAG C^TTAAG AocI 6326 CCTGAGG CC^T[ACGT]AGG ApoI 5966 GAATTC [GA]^AATT[CT] AseI 7794 ATTAAT AT^TAAT AsnI 7794 ATTAAT AT^TAAT Asp718I 1200 GGTACC G^GTACC AvrII 6988 CCTAGG C^CTAGG BamHI 5742 GGATCC G^GATCC BfrI 6611 CTTAAG C^TTAAG BlnI 6988 CCTAGG C^CTAGG BsiWI 165 CGTACG C^GTACG BssHII 1330 GCGCGC G^CGCGC BstEII 7367 GGTCACC G^GT[ACGT]ACC BstPI 7367 GGTCACC G^GT[ACGT]ACC Bsu36I 6326 CCTGAGG CC^T[ACGT]AGG CpoI 1010 CGGACCG CG^G[AT]CCG EagI 2164 CGGCCG C^GGCCG EclXI 2164 CGGCCG C^GGCCG Eco52I 2164 CGGCCG C^GGCCG Eco81I 6326 CCTGAGG CC^T[ACGT]AGG EcoO65I 7367 GGTCACC G^GT[ACGT]ACC EcoRI 5966 GAATTC G^AATTC KpnI 1200 GGTACC GGTAC^C MluI 1470 ACGCGT A^CGCGT NotI 2163 GCGGCCGC GC^GGCCGC PshBI 7794 ATTAAT AT^TAAT RsrII 1010 CGGACCG CG^G[AT]CCG SbfI 5110 CCTGCAGG CCTGCA^GG SexAI 444 ACCTGGT A^CC[AT]GGT SfiI 26 GGCCGCACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SpeI 7440 ACTAGT A^CTAGT Sse8387I 5110 CCTGCAGG CCTGCA^GG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccIII 1008 TCCGGA T^CCGGA 3588 TCCGGA AhdI 3194 GACGTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4346 GACAAGAAGTC AspEI 3194 GACGTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4346 GACAAGAAGTC AviII 876 TGCGCA TGC^GCA 4068 TGCGCA BbeI 2435 GGCGCC GGCGC^C 2892 GGCGCC BbvCI 5316 CCTCAGC CCTCAGC(-5/-2) 7023 GCTGAGG BcgI 3498 CGATTGTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 90 GCAATGACATCG BseAI 1008 TCCGGA T^CCGGA 3588 TCCGGA BspEI 1008 TCCGGA T^CCGGA 3588 TCCGGA BspLU11I 663 ACATGT A^CATGT 6702 ACATGT Eam1105I 3194 GACGTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4346 GACAAGAAGTC FspI 876 TGCGCA TGC^GCA 4068 TGCGCA KasI 2435 GGCGCC G^GCGCC 2892 GGCGCC MroI 1008 TCCGGA T^CCGGA 3588 TCCGGA NarI 2435 GGCGCC GG^CGCC 2892 GGCGCC PaeR7I 3536 CTCGAG C^TCGAG 6200 CTCGAG PshAI 859 GACCTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 5995 GACCAGTGTC SacI 959 GAGCTC GAGCT^C 6944 GAGCTC SalI 4475 GTCGAC G^TCGAC 6206 GTCGAC SfoI 2435 GGCGCC GGC^GCC 2892 GGCGCC SphI 2139 GCATGC GCATG^C 5580 GCATGC XhoI 3536 CTCGAG C^TCGAG 6200 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 160 enzymes) # ######################################### AccI AccII AciI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI Aor51HI ApaI ApaLI Asp700I AspHI AspI AvaI AvaII BalI BanI BanII BbrPI BbsI BbvI BclI BcnI BfaI BglI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DrdI DsaI EaeI EarI Eco47III EcoNI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflFI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RcaI RsaI SapI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII XmnI