##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
Alw44I ApaLI AscI Asp718I AspI
AviII AvrII BamHI BbrPI BbvCI
BfrI BlnI BsaWI BseAI BsiWI
BspDI BspEI BstBI ClaI CpoI
EcoRI EcoRV FseI FspI KpnI
MluI MroI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SalI SbfI SexAI SfuI SgrAI
SnaBI SpeI SphI Sse8387I Tth111I
XhoI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AatII 59 GACGTC GACGT^C
AhdI 3664 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 4190 AGCGCT AGC^GCT
ApaI 5063 GGGCCC GGGCC^C
AspEI 3664 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BciVI 1796 GTATCC GTATCC(6/5)
BglI 5317 GCCATGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 6255 GATCTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 362 CGGCCG CG[GA][CT]^CG
BsrDI 5016 GCAATG GCAATG(2/0)
BstEII 1541 GGTTACC G^GT[ACGT]ACC
BstPI 1541 GGTTACC G^GT[ACGT]ACC
DrdI 439 GACACCGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 362 CGGCCG C^GGCCG
Eam1105I 3664 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 362 CGGCCG C^GGCCG
Eco47III 4190 AGCGCT AGC^GCT
Eco52I 362 CGGCCG C^GGCCG
EcoNI 4883 CCTCTAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1541 GGTTACC G^GT[ACGT]ACC
MamI 6255 GATCTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1173 CAATTG C^AATTG
MunI 1173 CAATTG C^AATTG
NaeI 329 GCCGGC GCC^GGC
NdeI 1790 CATATG CA^TATG
NgoMIV 329 GCCGGC G^CCGGC
PflMI 2873 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 6401 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfiI 5316 GGCCATGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspI 6755 AATATT AAT^ATT
SwaI 6996 ATTTAAAT ATTT^AAAT
Van91I 2873 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AseI 5361 ATTAAT AT^TAAT
6507 ATTAAT
AsnI 5361 ATTAAT AT^TAAT
6507 ATTAAT
BbeI 251 GGCGCC GGCGC^C
5858 GGCGCC
BcgI 3312 CGAAAGCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1504 GCATTTCCCTCG
BglII 3414 AGATCT A^GATCT
6254 AGATCT
Bsp1407I 836 TGTACA T^GTACA
3906 TGTACA
BspLU11I 4471 ACATGT A^CATGT
4900 ACATGT
BsrBI 216 GAGCGG CCGCTC(-3/-3)
2854 GAGCGG
BsrFI 329 GCCGGC [GA]^CCGG[CT]
1481 ACCGGC
BsrGI 836 TGTACA T^GTACA
3906 TGTACA
BssHII 163 GCGCGC G^CGCGC
458 GCGCGC
BssSI 1200 CACGAG CACGAG(-5/-1)
1577 CTCGTG
Bst1107I 2344 GTATAC GTA^TAC
7219 GTATAC
BstAPI 543 GCACATCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5963 GCACCTTCTGC
BstZ17I 2344 GTATAC GTA^TAC
7219 GTATAC
Cfr10I 329 GCCGGC [GA]^CCGG[CT]
1481 ACCGGC
DraIII 2431 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
4984 CACCATGTG
EcoT22I 4515 ATGCAT ATGCA^T
4779 ATGCAT
HindIII 2067 AAGCTT A^AGCTT
3315 AAGCTT
KasI 251 GGCGCC G^GCGCC
5858 GGCGCC
KspI 147 CCGCGG CCGC^GG
377 CCGCGG
NarI 251 GGCGCC GG^CGCC
5858 GGCGCC
NcoI 422 CCATGG C^CATGG
4545 CCATGG
NheI 1978 GCTAGC G^CTAGC
6987 GCTAGC
NsiI 4515 ATGCAT ATGCA^T
4779 ATGCAT
PshBI 5361 ATTAAT AT^TAAT
6507 ATTAAT
SacII 147 CCGCGG CCGC^GG
377 CCGCGG
SapI 5657 GAAGAGC GCTCTTC(1/4)
132 GCTCTTC
ScaI 691 AGTACT AGT^ACT
3791 AGTACT
SfoI 251 GGCGCC GGC^GCC
5858 GGCGCC
SspBI 836 TGTACA T^GTACA
3906 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
AocI ApoI Asp700I AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BclI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspHI
BspMI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RcaI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XcmI
XhoII XmaCI XmaI XmnI