##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AccIII AhdI Aor51HI AscI
AseI AsnI AspEI AviII AvrII
BbeI BglI BlnI BlpI Bpu1102I
BseAI BsiEI BsiWI Bsp1407I BspEI
BspHI BsrBI BsrGI BssHII CelII
CpoI EagI Eam1105I EclXI Eco47III
Eco52I FseI FspI HpaI KasI
KspI MfeI MluI MroI MunI
NaeI NarI NgoMIV NotI NruI
PacI PmeI PshAI PshBI PvuI
RcaI RsrII SacII SalI SbfI
SexAI SfiI SfoI SgrAI SnaBI
SpeI Sse8387I SspBI StuI SwaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 1901 GGTACC G^GTACC
AcyI 6949 GGCGTC G[GA]^CG[CT]C
AflII 826 CTTAAG C^TTAAG
AgeI 6144 ACCGGT A^CCGGT
Asp718I 1901 GGTACC G^GTACC
BamHI 3770 GGATCC G^GATCC
BbrPI 1146 CACGTG CAC^GTG
BcgI 1316 GCATCAGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 826 CTTAAG C^TTAAG
BsaHI 6949 GGCGTC G[GA]^CG[CT]C
BsgI 6111 CTGCAC GTGCAG(16/14)
BspDI 2913 ATCGAT AT^CGAT
BssSI 6998 CTCGTG CACGAG(-5/-1)
Bst1107I 5313 GTATAC GTA^TAC
BstBI 5640 TTCGAA TT^CGAA
BstEII 1309 GGTCACC G^GT[ACGT]ACC
BstPI 1309 GGTCACC G^GT[ACGT]ACC
BstZ17I 5313 GTATAC GTA^TAC
ClaI 2913 ATCGAT AT^CGAT
EcoNI 5575 CCTCAGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1309 GGTCACC G^GT[ACGT]ACC
EcoRI 3274 GAATTC G^AATTC
EcoRV 2597 GATATC GAT^ATC
Hin1I 6949 GGCGTC G[GA]^CG[CT]C
HindIII 212 AAGCTT A^AGCTT
KpnI 1901 GGTACC GGTAC^C
NdeI 3780 CATATG CA^TATG
NspV 5640 TTCGAA TT^CGAA
PinAI 6144 ACCGGT A^CCGGT
PmaCI 1146 CACGTG CAC^GTG
PmlI 1146 CACGTG CAC^GTG
SapI 5481 GAAGAGC GCTCTTC(1/4)
SfuI 5640 TTCGAA TT^CGAA
SphI 2407 GCATGC GCATG^C
XbaI 6264 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 1186 CGCG CG^CG
4857 CGCG
AclI 1339 AACGTT AA^CGTT
5820 AACGTT
Alw44I 1143 GTGCAC G^TGCAC
5062 GTGCAC
ApaLI 1143 GTGCAC G^TGCAC
5062 GTGCAC
BciVI 349 GTATCC GTATCC(6/5)
2440 GTATCC
BglII 468 AGATCT A^GATCT
3306 AGATCT
BsaBI 3063 GATTTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3450 GATTTGAATC
BsaI 547 GGTCTC GGTCTC(1/5)
3834 GGTCTC
BseRI 892 CTCCTC GAGGAG(10/8)
4732 GAGGAG
BspLU11I 2322 ACATGT A^CATGT
3088 ACATGT
BstUI 1186 CGCG CG^CG
4857 CGCG
BstXI 3084 CCACACATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4445 CCAATCAGCTGG
DraI 2735 TTTAAA TTT^AAA
5664 TTTAAA
DraIII 1135 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
1535 CACTTGGTG
EcoT22I 1773 ATGCAT ATGCA^T
2373 ATGCAT
HaeII 1247 AGCGCC [GA]GCGC^[CT]
5465 AGCGCC
HincII 743 GTTGAC GT[CT]^[GA]AC
5050 GTCAAC
HindII 743 GTTGAC GT[CT]^[GA]AC
5050 GTCAAC
MamI 3063 GATTTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3450 GATTTGAATC
MvnI 1186 CGCG CG^CG
4857 CGCG
NcoI 1789 CCATGG C^CATGG
7116 CCATGG
NsiI 1773 ATGCAT ATGCA^T
2373 ATGCAT
PaeR7I 4015 CTCGAG C^TCGAG
7313 CTCGAG
PflMI 6852 CCATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
7111 CCAAACCATGG
Psp1406I 1339 AACGTT AA^CGTT
5820 AACGTT
SacI 957 GAGCTC GAGCT^C
5347 GAGCTC
Van91I 6852 CCATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
7111 CCAAACCATGG
XhoI 4015 CTCGAG C^TCGAG
7313 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
ApoI Asp700I AspHI AspI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BclI BcnI BfaI
BmyI BpmI BpuAI BsaAI BsaJI
BsaWI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrDI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DrdI DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NheI NlaIII NlaIV NspI
PflFI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmaI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
XcmI XhoII XmaCI XmaI XmnI