##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII AccI AccIII AflII AflIII
AgeI AhdI Alw44I AlwI Aor51HI
ApaLI AscI AseI AsnI Asp700I
AspEI AviII BalI BamHI BbvCI
BciVI BclI BfrI BsaBI BsaI
BsaWI BseAI BsiWI BsmI BspDI
BspEI BspHI BspLU11I BssHII Bst1107I
BstBI BstZ17I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KspI
MamI MfeI MluI MluNI MroI
MscI MunI NcoI NheI NotI
NruI NsiI NspV PacI PinAI
PmeI PshAI PshBI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspI SwaI
XbaI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 67 enzymes) #
###################################
AclI 1846 AACGTT AA^CGTT
AcyI 77 GGCGCC G[GA]^CG[CT]C
AocI 1523 CCTCAGG CC^T[ACGT]AGG
ApaI 323 GGGCCC GGGCC^C
AspI 236 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2005 CTCGAG C^[CT]CG[GA]G
AvrII 1145 CCTAGG C^CTAGG
BbeI 77 GGCGCC GGCGC^C
BbrPI 1800 CACGTG CAC^GTG
BbsI 404 GAAGAC GAAGAC(2/6)
BcgI 178 CGACTGTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1644 AGATCT A^GATCT
BlnI 1145 CCTAGG C^CTAGG
BlpI 1151 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1151 GCTGAGC GC^T[ACGT]AGC
BpuAI 404 GAAGAC GAAGAC(2/6)
BsaAI 1800 CACGTG [CT]AC^GT[GA]
BsaHI 77 GGCGCC G[GA]^CG[CT]C
BsgI 1269 CTGCAC GTGCAG(16/14)
BsiEI 1542 CGACCG CG[GA][CT]^CG
BsoBI 2005 CTCGAG C^[CT]CG[GA]G
Bsp1407I 704 TGTACA T^GTACA
BsrBI 627 CCGCTC CCGCTC(-3/-3)
BsrDI 1721 CATTGC GCAATG(2/0)
BsrGI 704 TGTACA T^GTACA
BssSI 609 CACGAG CACGAG(-5/-1)
BstAPI 126 GCAAGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1123 GGTGACC G^GT[ACGT]ACC
BstPI 1123 GGTGACC G^GT[ACGT]ACC
BstXI 1465 CCAGCTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1644 AGATCT [GA]^GATC[CT]
Bsu36I 1523 CCTCAGG CC^T[ACGT]AGG
CelII 1151 GCTGAGC GC^T[ACGT]AGC
DraIII 1062 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 318 CCACGG C^C[GA][CT]GG
EaeI 715 TGGCCG [CT]^GGCC[GA]
EarI 143 CTCTTC CTCTTC(1/4)
Eco81I 1523 CCTCAGG CC^T[ACGT]AGG
EcoNI 443 CCTCGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1123 GGTGACC G^GT[ACGT]ACC
EcoRI 1013 GAATTC G^AATTC
HaeII 77 GGCGCC [GA]GCGC^[CT]
HgaI 174 GACGC GACGC(5/10)
Hin1I 77 GGCGCC G[GA]^CG[CT]C
KasI 77 GGCGCC G^GCGCC
Ksp632I 143 CTCTTC CTCTTC(1/4)
MflI 1644 AGATCT [GA]^GATC[CT]
MslI 1865 CACTGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 77 GGCGCC GG^CGCC
NdeI 1810 CATATG CA^TATG
NspI 1794 GCATGC [GA]CATG^[CT]
PaeR7I 2005 CTCGAG C^TCGAG
PflFI 236 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1800 CACGTG CAC^GTG
PmlI 1800 CACGTG CAC^GTG
Psp1406I 1846 AACGTT AA^CGTT
PstI 386 CTGCAG CTGCA^G
SexAI 1163 ACCTGGT A^CC[AT]GGT
SfiI 615 GGCCTCAAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 77 GGCGCC GGC^GCC
SphI 1794 GCATGC GCATG^C
SspBI 704 TGTACA T^GTACA
StuI 129 AGGCCT AGG^CCT
Tth111I 236 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 911 CCACTTGTCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2005 CTCGAG C^TCGAG
XhoII 1644 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Acc65I 928 GGTACC G^GTACC
1167 GGTACC
AccII 42 CGCG CG^CG
176 CGCG
AcsI 981 AAATTC [GA]^AATT[CT]
1013 GAATTC
AlwNI 733 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
1918 CAGCTCCTG
ApoI 981 AAATTC [GA]^AATT[CT]
1013 GAATTC
Asp718I 928 GGTACC G^GTACC
1167 GGTACC
AspHI 1137 GAGCAC G[AT]GC[AT]^C
2080 GTGCTC
BsiHKAI 1137 GAGCAC G[AT]GC[AT]^C
2080 GTGCTC
BsmBI 1284 CGTCTC CGTCTC(1/5)
172 GAGACG
BstUI 42 CGCG CG^CG
176 CGCG
DpnII 553 GATC ^GATC
1645 GATC
EcoT14I 292 CCTTGG C^C[AT][AT]GG
1145 CCTAGG
KpnI 928 GGTACC GGTAC^C
1167 GGTACC
MaeII 1801 ACGT A^CGT
1847 ACGT
MboI 553 GATC ^GATC
1645 GATC
MvnI 42 CGCG CG^CG
176 CGCG
NaeI 546 GCCGGC GCC^GGC
881 GCCGGC
NdeII 553 GATC ^GATC
1645 GATC
NgoMIV 546 GCCGGC G^CCGGC
881 GCCGGC
PflMI 732 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1578 CCATGTGGTGG
Sau3AI 553 GATC ^GATC
1645 GATC
SfcI 386 CTGCAG C^T[GA][CT]AG
594 CTGTAG
StyI 292 CCTTGG C^C[AT][AT]GG
1145 CCTAGG
TfiI 46 GATTC G^A[AT]TC
2093 GATTC
Van91I 732 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1578 CCATGTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AfaI AluI AvaII BanI
BanII BbvI BcnI BfaI BglI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr10I Cfr13I DdeI
DraI DraII EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeIII MboII MnlI MseI
MspA1I MspI MvaI MwoI NciI
NlaIII NlaIV PleI PpuMI PvuII
RsaI Sau96I ScrFI SfaNI SmlI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I