##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII AclI AcyI AflII AgeI
Alw44I ApaLI AscI AspI AviII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BfrI BglI BsaAI BsaBI
BsaHI BsaI BsiWI BsmAI BsmBI
Bsp1407I BspDI BspMI BsrBI BsrGI
BssHII BssSI BstXI ClaI CpoI
DraIII EarI EcoNI EcoT22I FseI
FspI HgaI Hin1I HpaI KasI
Ksp632I KspI MamI MfeI MluI
MunI NarI NdeI NheI NruI
NsiI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
Psp1406I PvuI RsrII SacII SapI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccIII 1120 TCCGGA T^CCGGA
AhdI 1124 GACAGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 1063 GGATC GGATC(4/5)
AocI 658 CCTCAGG CC^T[ACGT]AGG
Aor51HI 342 AGCGCT AGC^GCT
ApaI 894 GGGCCC GGGCC^C
Asp700I 2021 GAATACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1124 GACAGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 62 CCTAGG C^CTAGG
BclI 1984 TGATCA T^GATCA
BlnI 62 CCTAGG C^CTAGG
BlpI 1591 GCTGAGC GC^T[ACGT]AGC
BpmI 1361 CTCCAG CTGGAG(16/14)
Bpu1102I 1591 GCTGAGC GC^T[ACGT]AGC
BsaWI 1120 TCCGGA [AT]^CCGG[AT]
BseAI 1120 TCCGGA T^CCGGA
BsgI 530 GTGCAG GTGCAG(16/14)
BspEI 1120 TCCGGA T^CCGGA
BspHI 691 TCATGA T^CATGA
BspLU11I 2258 ACATGT A^CATGT
BsrDI 1008 CATTGC GCAATG(2/0)
BsrFI 39 GCCGGC [GA]^CCGG[CT]
BstEII 1612 GGTCACC G^GT[ACGT]ACC
BstPI 1612 GGTCACC G^GT[ACGT]ACC
Bsu36I 658 CCTCAGG CC^T[ACGT]AGG
CelII 1591 GCTGAGC GC^T[ACGT]AGC
Cfr10I 39 GCCGGC [GA]^CCGG[CT]
DrdI 1667 GACCACACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1124 GACAGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 342 AGCGCT AGC^GCT
Eco81I 658 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1612 GGTCACC G^GT[ACGT]ACC
EcoRI 507 GAATTC G^AATTC
EcoRV 2053 GATATC GAT^ATC
FbaI 1984 TGATCA T^GATCA
HindIII 1292 AAGCTT A^AGCTT
MaeII 49 ACGT A^CGT
MroI 1120 TCCGGA T^CCGGA
NaeI 39 GCCGGC GCC^GGC
NgoMIV 39 GCCGGC G^CCGGC
NotI 202 GCGGCCGC GC^GGCCGC
PshAI 770 GACTTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 586 CTGCAG CTGCA^G
RcaI 691 TCATGA T^CATGA
SacI 1358 GAGCTC GAGCT^C
SalI 323 GTCGAC G^TCGAC
SmlI 2378 CTTGAG C^T[CT][GA]AG
XbaI 2234 TCTAGA T^CTAGA
XmnI 2021 GAATACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 827 GGTACC G^GTACC
1653 GGTACC
AccII 58 CGCG CG^CG
312 CGCG
AflIII 49 ACGTGT A^C[GA][CT]GT
2258 ACATGT
AlwNI 161 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
1203 CAGCTGCTG
AseI 740 ATTAAT AT^TAAT
2604 ATTAAT
AsnI 740 ATTAAT AT^TAAT
2604 ATTAAT
Asp718I 827 GGTACC G^GTACC
1653 GGTACC
AvaI 218 CCCGAG C^[CT]CG[GA]G
295 CTCGGG
AvaII 31 GGACC G^G[AT]CC
2176 GGACC
BalI 1753 TGGCCA TGG^CCA
2582 TGGCCA
BanII 894 GGGCCC G[GA]GC[CT]^C
1358 GAGCTC
BcnI 259 CCCGG CC^[GC]GG
352 CCGGG
BsiEI 37 CGGCCG CG[GA][CT]^CG
203 CGGCCG
BsoBI 218 CCCGAG C^[CT]CG[GA]G
295 CTCGGG
Bst1107I 913 GTATAC GTA^TAC
991 GTATAC
BstBI 1887 TTCGAA TT^CGAA
2169 TTCGAA
BstUI 58 CGCG CG^CG
312 CGCG
BstZ17I 913 GTATAC GTA^TAC
991 GTATAC
EagI 37 CGGCCG C^GGCCG
203 CGGCCG
EclXI 37 CGGCCG C^GGCCG
203 CGGCCG
Eco52I 37 CGGCCG C^GGCCG
203 CGGCCG
HaeII 163 GGCGCT [GA]GCGC^[CT]
342 AGCGCT
KpnI 827 GGTACC GGTAC^C
1653 GGTACC
MluNI 1753 TGGCCA TGG^CCA
2582 TGGCCA
MscI 1753 TGGCCA TGG^CCA
2582 TGGCCA
MvnI 58 CGCG CG^CG
312 CGCG
NciI 259 CCCGG CC^[GC]GG
352 CCGGG
NspV 1887 TTCGAA TT^CGAA
2169 TTCGAA
PshBI 740 ATTAAT AT^TAAT
2604 ATTAAT
SfuI 1887 TTCGAA TT^CGAA
2169 TTCGAA
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
ApoI AspHI BanI BbsI BbvI
BfaI BglII BmyI BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmFI
BsmI Bsp1286I BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NcoI NdeII NlaIII NlaIV NspI
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII