##################################
# NO CUT ENZYMES ( 39 enzymes) #
##################################
AatII Acc65I AccI AflII AgeI
AscI Asp718I BfrI BglII BsiWI
BspDI BssHII Bst1107I BstZ17I ClaI
CpoI EcoRV FseI HindIII KpnI
MfeI MluI MunI NdeI NotI
NruI PinAI PmeI PvuI RsrII
SalI SbfI ScaI SfiI SgrAI
SpeI Sse8387I SwaI XbaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AflIII 1052 ACATGT A^C[GA][CT]GT
AviII 1404 TGCGCA TGC^GCA
AvrII 2358 CCTAGG C^CTAGG
BclI 897 TGATCA T^GATCA
BlnI 2358 CCTAGG C^CTAGG
BlpI 466 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 466 GCTGAGC GC^T[ACGT]AGC
BsaBI 6336 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspLU11I 1052 ACATGT A^CATGT
BssSI 812 CACGAG CACGAG(-5/-1)
BstBI 1810 TTCGAA TT^CGAA
BstEII 5646 GGTAACC G^GT[ACGT]ACC
BstPI 5646 GGTAACC G^GT[ACGT]ACC
CelII 466 GCTGAGC GC^T[ACGT]AGC
EagI 2262 CGGCCG C^GGCCG
EclXI 2262 CGGCCG C^GGCCG
Eco52I 2262 CGGCCG C^GGCCG
EcoO65I 5646 GGTAACC G^GT[ACGT]ACC
EcoT22I 4726 ATGCAT ATGCA^T
FbaI 897 TGATCA T^GATCA
FspI 1404 TGCGCA TGC^GCA
HpaI 4801 GTTAAC GTT^AAC
MamI 6336 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NsiI 4726 ATGCAT ATGCA^T
NspV 1810 TTCGAA TT^CGAA
PacI 3095 TTAATTAA TTAAT^TAA
PaeR7I 16 CTCGAG C^TCGAG
PshAI 2077 GACGGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 615 ACCTGGT A^CC[AT]GGT
SfuI 1810 TTCGAA TT^CGAA
SnaBI 778 TACGTA TAC^GTA
SphI 4724 GCATGC GCATG^C
XhoI 16 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccIII 484 TCCGGA T^CCGGA
3324 TCCGGA
AhdI 2155 GACATCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2861 GACTTTGTGTC
AocI 3545 CCTAAGG CC^T[ACGT]AGG
4250 CCTCAGG
ApaI 2239 GGGCCC GGGCC^C
4345 GGGCCC
AspEI 2155 GACATCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2861 GACTTTGTGTC
AspI 614 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1673 GACGATGTC
BamHI 1780 GGATCC G^GATCC
2760 GGATCC
BcgI 5253 CGACCGCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2801 GCACTGGGCTCG
BciVI 4882 GGATAC GTATCC(6/5)
2411 GTATCC
BglI 5085 GCCTCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5145 GCCCGACAGGC
BseAI 484 TCCGGA T^CCGGA
3324 TCCGGA
BsiEI 2262 CGGCCG CG[GA][CT]^CG
5253 CGACCG
BsmBI 194 CGTCTC CGTCTC(1/5)
1484 GAGACG
BsmI 1813 GAATGC GAATGC(1/-1)
6325 GCATTC
Bsp1407I 3266 TGTACA T^GTACA
4987 TGTACA
BspEI 484 TCCGGA T^CCGGA
3324 TCCGGA
BsrGI 3266 TGTACA T^GTACA
4987 TGTACA
BstXI 609 CCATGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5693 CCAGGAGCATGG
Bsu36I 3545 CCTAAGG CC^T[ACGT]AGG
4250 CCTCAGG
DraIII 6274 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
7346 CACGTGGTG
DrdI 1486 GACGGGATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5028 GACAGTGATGTC
Eam1105I 2155 GACATCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2861 GACTTTGTGTC
Eco81I 3545 CCTAAGG CC^T[ACGT]AGG
4250 CCTCAGG
EcoNI 616 CCTGGTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3304 CCTTTGAAAGG
HincII 4443 GTTGAC GT[CT]^[GA]AC
4801 GTTAAC
HindII 4443 GTTGAC GT[CT]^[GA]AC
4801 GTTAAC
KspI 129 CCGCGG CCGC^GG
1785 CCGCGG
MroI 484 TCCGGA T^CCGGA
3324 TCCGGA
PflFI 614 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1673 GACGATGTC
PpuMI 2354 GGGACCT [GA]G^G[AT]CC[CT]
5162 GGGTCCC
SacI 2487 GAGCTC GAGCT^C
6306 GAGCTC
SacII 129 CCGCGG CCGC^GG
1785 CCGCGG
SapI 2275 GAAGAGC GCTCTTC(1/4)
2751 GAAGAGC
SspBI 3266 TGTACA T^GTACA
4987 TGTACA
SspI 3521 AATATT AAT^ATT
6963 AATATT
StuI 603 AGGCCT AGG^CCT
3964 AGGCCT
Tth111I 614 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1673 GACGATGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 149 enzymes) #
#########################################
AccII AciI AclI AcsI AcyI
AfaI AluI Alw44I AlwI AlwNI
Aor51HI ApaLI ApoI AseI AsnI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbeI BbrPI BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI Eco47III
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NcoI
NdeII NgoMIV NheI NlaIII NlaIV
NspI PflMI PleI PmaCI PmlI
PshBI Psp1406I PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI XmnI