##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
Acc65I AccIII AclI AgeI AocI
AscI AseI AsnI Asp718I AvrII
BciVI BlnI BsaWI BseAI BspDI
BspEI BssHII Bst1107I BstBI BstZ17I
Bsu36I ClaI Eco81I EcoRV EcoT22I
FseI HindIII HpaI KpnI MfeI
MluI MroI MunI NheI NotI
NruI NsiI NspV PacI PinAI
PmeI PshAI PshBI Psp1406I PvuI
SalI ScaI SfuI SgrAI SnaBI
SpeI SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AflII 5441 CTTAAG C^TTAAG
Alw44I 586 GTGCAC G^TGCAC
ApaLI 586 GTGCAC G^TGCAC
Asp700I 4087 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 3890 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BfrI 5441 CTTAAG C^TTAAG
BlpI 4045 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4045 GCTGAGC GC^T[ACGT]AGC
BsaBI 92 GATAAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 648 CGTACG C^GTACG
Bsp1407I 483 TGTACA T^GTACA
BspLU11I 1023 ACATGT A^CATGT
BsrGI 483 TGTACA T^GTACA
CelII 4045 GCTGAGC GC^T[ACGT]AGC
CpoI 5588 CGGACCG CG^G[AT]CCG
DraIII 6428 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 5790 CGGCCG C^GGCCG
EclXI 5790 CGGCCG C^GGCCG
Eco52I 5790 CGGCCG C^GGCCG
KspI 5145 CCGCGG CCGC^GG
MamI 92 GATAAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 7158 CATATG CA^TATG
PflFI 3890 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsrII 5588 CGGACCG CG^G[AT]CCG
SacII 5145 CCGCGG CCGC^GG
SexAI 1938 ACCAGGT A^CC[AT]GGT
SphI 5022 GCATGC GCATG^C
SspBI 483 TGTACA T^GTACA
Tth111I 3890 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 4087 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AatII 2414 GACGTC GACGT^C
6374 GACGTC
AccI 6834 GTCTAC GT^[AC][GT]AC
7027 GTCTAC
Aor51HI 2859 AGCGCT AGC^GCT
4884 AGCGCT
AviII 1365 TGCGCA TGC^GCA
3873 TGCGCA
BamHI 1419 GGATCC G^GATCC
1840 GGATCC
BbeI 1403 GGCGCC GGCGC^C
5885 GGCGCC
BbrPI 340 CACGTG CAC^GTG
6428 CACGTG
BclI 3156 TGATCA T^GATCA
5313 TGATCA
BsmBI 2687 GAGACG CGTCTC(1/5)
5786 GAGACG
BsmI 5404 GAATGC GAATGC(1/-1)
1641 GCATTC
BspHI 2298 TCATGA T^CATGA
7136 TCATGA
DraI 6890 TTTAAA TTT^AAA
7113 TTTAAA
DrdI 1886 GACAAGTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2689 GACGCTGCAGTC
Eco47III 2859 AGCGCT AGC^GCT
4884 AGCGCT
EcoRI 4087 GAATTC G^AATTC
7177 GAATTC
FbaI 3156 TGATCA T^GATCA
5313 TGATCA
FspI 1365 TGCGCA TGC^GCA
3873 TGCGCA
HincII 4751 GTCAAC GT[CT]^[GA]AC
6875 GTTGAC
HindII 4751 GTCAAC GT[CT]^[GA]AC
6875 GTTGAC
KasI 1403 GGCGCC G^GCGCC
5885 GGCGCC
NarI 1403 GGCGCC GG^CGCC
5885 GGCGCC
PmaCI 340 CACGTG CAC^GTG
6428 CACGTG
PmlI 340 CACGTG CAC^GTG
6428 CACGTG
RcaI 2298 TCATGA T^CATGA
7136 TCATGA
SfoI 1403 GGCGCC GGC^GCC
5885 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 149 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AhdI AluI AlwI AlwNI
ApaI ApoI AspEI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcgI BcnI BfaI
BglI BglII BmyI BpmI BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BssSI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI Eam1105I EarI
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PaeR7I
PflMI PleI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
SbfI ScrFI SfaNI SfcI SfiI
SmaI SmlI Sse8387I StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoI XhoII XmaCI XmaI