##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII AccII AclI AcyI AflII
AgeI AhdI Alw44I AocI ApaI
ApaLI AscI AseI AsnI AspEI
AspI AviII BalI BamHI BbeI
BbrPI BbvCI BcgI BciVI BfrI
BglI BsaBI BsaHI BsaI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspHI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I ClaI CpoI DraII DrdI
EagI Eam1105I EclXI Eco52I Eco81I
EcoO109I EcoO65I EcoRI EcoRV FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI KspI MamI MluI
MluNI MscI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI Sse8387I SspBI StuI
SwaI Tth111I Van91I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 1954 GGTACC G^GTACC
AccI 1639 GTCTAC GT^[AC][GT]AC
AccIII 1486 TCCGGA T^CCGGA
AflIII 2396 ACATGT A^C[GA][CT]GT
AlwI 1099 GATCC GGATC(4/5)
AlwNI 2135 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 166 AGCGCT AGC^GCT
Asp718I 1954 GGTACC G^GTACC
AvaI 92 CCCGGG C^[CT]CG[GA]G
AvrII 7 CCTAGG C^CTAGG
BclI 2298 TGATCA T^GATCA
BlnI 7 CCTAGG C^CTAGG
BlpI 1544 GCTCAGC GC^T[ACGT]AGC
BpmI 1519 CTCCAG CTGGAG(16/14)
Bpu1102I 1544 GCTCAGC GC^T[ACGT]AGC
BsaWI 1486 TCCGGA [AT]^CCGG[AT]
BseAI 1486 TCCGGA T^CCGGA
BsgI 1990 GTGCAG GTGCAG(16/14)
BsoBI 92 CCCGGG C^[CT]CG[GA]G
BspEI 1486 TCCGGA T^CCGGA
BspLU11I 2396 ACATGT A^CATGT
BsrBI 852 CCGCTC CCGCTC(-3/-3)
BsrFI 1298 ACCGGC [GA]^CCGG[CT]
CelII 1544 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1298 ACCGGC [GA]^CCGG[CT]
DraIII 2362 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 166 AGCGCT AGC^GCT
EcoT22I 1195 ATGCAT ATGCA^T
FauI 88 CCCGC CCCGC(4/6)
FbaI 2298 TGATCA T^GATCA
HaeII 166 AGCGCT [GA]GCGC^[CT]
KpnI 1954 GGTACC GGTAC^C
MfeI 314 CAATTG C^AATTG
MroI 1486 TCCGGA T^CCGGA
MunI 314 CAATTG C^AATTG
MwoI 2002 GCCTTAGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NsiI 1195 ATGCAT ATGCA^T
PstI 1916 CTGCAG CTGCA^G
ScaI 1907 AGTACT AGT^ACT
SexAI 1463 ACCAGGT A^CC[AT]GGT
SmaI 92 CCCGGG CCC^GGG
SphI 1197 GCATGC GCATG^C
SspI 892 AATATT AAT^ATT
XcmI 1083 CCATGTGTACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 92 CCCGGG C^CCGGG
XmaI 92 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Asp700I 382 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
535 GAAGGTATTC
AvaII 1268 GGACC G^G[AT]CC
1887 GGACC
BanI 724 GGTGCC G^G[CT][GA]CC
1954 GGTACC
BbsI 117 GTCTTC GAAGAC(2/6)
884 GTCTTC
BglII 425 AGATCT A^GATCT
968 AGATCT
BpuAI 117 GTCTTC GAAGAC(2/6)
884 GTCTTC
BsaAI 1661 TACGTG [CT]AC^GT[GA]
2185 CACGTA
BsmI 1424 GCATTC GAATGC(1/-1)
1434 GCATTC
BstXI 1359 CCAGGCATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2329 CCACCACAGTGG
CfoI 167 GCGC GCG^C
1581 GCGC
EaeI 1300 CGGCCA [CT]^GGCC[GA]
1418 CGGCCA
EcoNI 405 CCTCCGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2012 CCTGTGTGAGG
HgaI 2281 GACGC GACGC(5/10)
453 GCGTC
HhaI 167 GCGC GCG^C
1581 GCGC
HinP1I 167 GCGC G^CGC
1581 GCGC
MaeII 1662 ACGT A^CGT
2186 ACGT
MspA1I 1124 CAGCTG C[AC]G^C[GT]G
1561 CAGCTG
NcoI 110 CCATGG C^CATGG
1307 CCATGG
PvuII 1124 CAGCTG CAG^CTG
1561 CAGCTG
SacI 193 GAGCTC GAGCT^C
1143 GAGCTC
SfcI 1641 CTACAG C^T[GA][CT]AG
1916 CTGCAG
XmnI 382 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
535 GAAGGTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AspHI BanII BbvI BcnI BfaI
BmyI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DsaI EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI NciI
NdeII NlaIII NlaIV NspI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII