##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
Aor51HI AscI AseI AsnI Asp718I
BamHI BfrI BglII BsaBI BsiWI
BsmBI BspDI BspHI BspLU11I Bst1107I
BstBI BstZ17I ClaI CpoI DraI
Eco47III EcoRV EcoT22I HindIII HpaI
KpnI MamI MfeI MluI MunI
NdeI NheI NsiI NspV PacI
PinAI PmeI PshBI Psp1406I RcaI
RsrII SalI ScaI SfuI SgrAI
SpeI SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 15 enzymes) #
###################################
AviII 5732 TGCGCA TGC^GCA
AvrII 7205 CCTAGG C^CTAGG
BclI 320 TGATCA T^GATCA
BlnI 7205 CCTAGG C^CTAGG
DrdI 1117 GACGACGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 320 TGATCA T^GATCA
FseI 45 GGCCGGCC GGCCGG^CC
FspI 5732 TGCGCA TGC^GCA
MseI 7137 TTAA T^TAA
NruI 3106 TCGCGA TCG^CGA
PvuI 1156 CGATCG CGAT^CG
SacI 6854 GAGCTC GAGCT^C
SfiI 5056 GGCCCCAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 1775 GCATGC GCATG^C
Tru9I 7137 TTAA T^TAA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 3576 GAATTC [GA]^AATT[CT]
4237 GAATTC
Alw44I 1786 GTGCAC G^TGCAC
3658 GTGCAC
ApaLI 1786 GTGCAC G^TGCAC
3658 GTGCAC
ApoI 3576 GAATTC [GA]^AATT[CT]
4237 GAATTC
Asp700I 963 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3591 GAAGGATTTC
BciVI 5634 GGATAC GTATCC(6/5)
6809 GTATCC
Bsp1407I 2572 TGTACA T^GTACA
4271 TGTACA
BsrGI 2572 TGTACA T^GTACA
4271 TGTACA
DraIII 2220 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
3141 CACCGTGTG
EcoRI 3576 GAATTC G^AATTC
4237 GAATTC
KspI 756 CCGCGG CCGC^GG
2705 CCGCGG
NotI 72 GCGGCCGC GC^GGCCGC
3756 GCGGCCGC
NspI 130 ACATGC [GA]CATG^[CT]
1775 GCATGC
PaeR7I 1524 CTCGAG C^TCGAG
3295 CTCGAG
SacII 756 CCGCGG CCGC^GG
2705 CCGCGG
SapI 636 GAAGAGC GCTCTTC(1/4)
4806 GAAGAGC
SbfI 1512 CCTGCAGG CCTGCA^GG
5937 CCTGCAGG
SexAI 275 ACCTGGT A^CC[AT]GGT
356 ACCAGGT
SnaBI 2269 TACGTA TAC^GTA
5332 TACGTA
Sse8387I 1512 CCTGCAGG CCTGCA^GG
5937 CCTGCAGG
SspBI 2572 TGTACA T^GTACA
4271 TGTACA
StuI 4989 AGGCCT AGG^CCT
6062 AGGCCT
XhoI 1524 CTCGAG C^TCGAG
3295 CTCGAG
XmnI 963 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3591 GAAGGATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 165 enzymes) #
#########################################
AatII AccII AccIII AciI AcyI
AfaI AflIII AhdI AluI AlwI
AlwNI AocI ApaI AspEI AspHI
AspI AvaI AvaII BalI BanI
BanII BbeI BbrPI BbsI BbvCI
BbvI BcgI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BsrI BssHII BssKI
BssSI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI Eam1105I EarI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MroI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NcoI NdeII
NgoMIV NlaIII NlaIV PflFI PflMI
PleI PmaCI PmlI PpuMI PshAI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SmlI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XcmI XhoII XmaCI XmaI