##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
Acc65I AclI Alw44I ApaLI AscI
AseI AsnI Asp718I AspI AvrII
BbeI BbsI BbvCI BcgI BciVI
BclI BlnI BpuAI BsaBI BsaI
BsiWI BsmAI BsmBI Bsp1407I BspDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI DraIII DrdI EagI EclXI
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MunI NarI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PmeI PshBI Psp1406I PvuI
RsrII SacII SexAI SfoI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 1784 GACGTC GACGT^C
AccI 2374 GTCGAC GT^[AC][GT]AC
AccIII 319 TCCGGA T^CCGGA
AcsI 2448 AAATTT [GA]^AATT[CT]
AflII 128 CTTAAG C^TTAAG
AgeI 2331 ACCGGT A^CCGGT
AocI 1056 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1756 AGCGCT AGC^GCT
ApoI 2448 AAATTT [GA]^AATT[CT]
AviII 2113 TGCGCA TGC^GCA
BbrPI 1732 CACGTG CAC^GTG
BfaI 393 CTAG C^TAG
BfrI 128 CTTAAG C^TTAAG
BglI 950 GCCCCGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1696 AGATCT A^GATCT
BlpI 2215 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2215 GCTGAGC GC^T[ACGT]AGC
BseAI 319 TCCGGA T^CCGGA
BsiEI 2210 CGACCG CG[GA][CT]^CG
BspEI 319 TCCGGA T^CCGGA
BspHI 271 TCATGA T^CATGA
BspLU11I 2378 ACATGT A^CATGT
BstAPI 1637 GCAGGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 611 CCAGGTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1056 CCTGAGG CC^T[ACGT]AGG
CelII 2215 GCTGAGC GC^T[ACGT]AGC
Eco47III 1756 AGCGCT AGC^GCT
Eco81I 1056 CCTGAGG CC^T[ACGT]AGG
FspI 2113 TGCGCA TGC^GCA
MaeI 393 CTAG C^TAG
MroI 319 TCCGGA T^CCGGA
MslI 2117 CACAGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflMI 1367 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2331 ACCGGT A^CCGGT
PmaCI 1732 CACGTG CAC^GTG
PmlI 1732 CACGTG CAC^GTG
PshAI 786 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 271 TCATGA T^CATGA
SacI 1968 GAGCTC GAGCT^C
SalI 2374 GTCGAC G^TCGAC
SapI 1515 GAAGAGC GCTCTTC(1/4)
SbfI 152 CCTGCAGG CCTGCA^GG
ScaI 898 AGTACT AGT^ACT
SfiI 949 GGCCCCGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 152 CCTGCAGG CCTGCA^GG
StuI 486 AGGCCT AGG^CCT
Tsp509I 2449 AATT ^AATT
Van91I 1367 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1781 CCAGACGTCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflIII 1733 ACGTGT A^C[GA][CT]GT
2378 ACATGT
AhdI 201 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1350 GACCCTGCGTC
Asp700I 818 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1539 GAACGCATTC
AspEI 201 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1350 GACCCTGCGTC
BalI 337 TGGCCA TGG^CCA
598 TGGCCA
BsrBI 426 GAGCGG CCGCTC(-3/-3)
441 GAGCGG
Eam1105I 201 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1350 GACCCTGCGTC
EcoNI 152 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1115 CCTGTATCAGG
HincII 2335 GTTGAC GT[CT]^[GA]AC
2374 GTCGAC
HindII 2335 GTTGAC GT[CT]^[GA]AC
2374 GTCGAC
MluNI 337 TGGCCA TGG^CCA
598 TGGCCA
MscI 337 TGGCCA TGG^CCA
598 TGGCCA
MseI 129 TTAA T^TAA
2442 TTAA
NaeI 856 GCCGGC GCC^GGC
955 GCCGGC
NcoI 305 CCATGG C^CATGG
1151 CCATGG
NgoMIV 856 GCCGGC G^CCGGC
955 GCCGGC
PvuII 873 CAGCTG CAG^CTG
1924 CAGCTG
SmaI 460 CCCGGG CCC^GGG
883 CCCGGG
SmlI 128 CTTAAG C^T[CT][GA]AG
1608 CTTGAG
TaqI 1537 TCGA T^CGA
2375 TCGA
Tru9I 129 TTAA T^TAA
2442 TTAA
TthHB8I 1537 TCGA T^CGA
2375 TCGA
XmaCI 460 CCCGGG C^CCGGG
883 CCCGGG
XmaI 460 CCCGGG C^CCGGG
883 CCCGGG
XmnI 818 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1539 GAACGCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BamHI BanI BanII BbvI
BcnI BmyI BpmI BsaAI BsaHI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TfiI
TseI Tsp45I TspRI XhoII