##################################
# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII AccII AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspI
AviII BamHI BbeI BbrPI BbsI
BbvCI BcgI BclI BfrI BglI
BpuAI BsaAI BsaBI BsaWI BseAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CfoI ClaI
CpoI DraIII Eam1105I Eco47III Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HhaI HinP1I
HindIII KasI KspI MamI MfeI
MluI MroI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SwaI Tth111I
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 430 GGTACC G^GTACC
AcyI 78 GACGCC G[GA]^CG[CT]C
ApaI 477 GGGCCC GGGCC^C
Asp718I 430 GGTACC G^GTACC
AvrII 1268 CCTAGG C^CTAGG
BalI 1608 TGGCCA TGG^CCA
BlnI 1268 CCTAGG C^CTAGG
BlpI 739 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 739 GCTGAGC GC^T[ACGT]AGC
BsaHI 78 GACGCC G[GA]^CG[CT]C
BsaI 414 GAGACC GGTCTC(1/5)
BseRI 770 GAGGAG GAGGAG(10/8)
BsgI 1995 CTGCAC GTGCAG(16/14)
BsiEI 226 CGGCCG CG[GA][CT]^CG
BsrDI 1591 CATTGC GCAATG(2/0)
BsrFI 701 GCCGGT [GA]^CCGG[CT]
BstAPI 692 GCAGCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 739 GCTGAGC GC^T[ACGT]AGC
Cfr10I 701 GCCGGT [GA]^CCGG[CT]
DraI 1568 TTTAAA TTT^AAA
EagI 226 CGGCCG C^GGCCG
EclXI 226 CGGCCG C^GGCCG
Eco52I 226 CGGCCG C^GGCCG
FauI 600 CCCGC CCCGC(4/6)
HgaI 78 GACGC GACGC(5/10)
Hin1I 78 GACGCC G[GA]^CG[CT]C
HpaI 2111 GTTAAC GTT^AAC
KpnI 430 GGTACC GGTAC^C
MaeII 1764 ACGT A^CGT
MluNI 1608 TGGCCA TGG^CCA
MscI 1608 TGGCCA TGG^CCA
NspI 1106 ACATGC [GA]CATG^[CT]
PaeR7I 555 CTCGAG C^TCGAG
PflMI 656 CCAGTACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 791 AGGTCCC [GA]G^G[AT]CC[CT]
PstI 2091 CTGCAG CTGCA^G
ScaI 436 AGTACT AGT^ACT
SexAI 426 ACCAGGT A^CC[AT]GGT
SspI 2098 AATATT AAT^ATT
StuI 1937 AGGCCT AGG^CCT
Van91I 656 CCAGTACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 812 CCAGGGTAGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 555 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 630 GTCTAC GT^[AC][GT]AC
817 GTAGAC
AlwI 680 GATCC GGATC(4/5)
2223 GATCC
AlwNI 776 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
2325 CAGAACCTG
BciVI 2177 GGATAC GTATCC(6/5)
1065 GTATCC
BglII 2236 AGATCT A^GATCT
2433 AGATCT
BpmI 1943 CTCCAG CTGGAG(16/14)
542 CTGGAG
BsmI 1774 GAATGC GAATGC(1/-1)
1530 GCATTC
BstF5I 2104 CATCC GGATG(2/0)
1164 GGATG
BstXI 63 CCACCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1091 CCAGGCCAATGG
DraII 476 GGGGCCC [GA]G^G[ACGT]CC[CT]
791 AGGTCCC
DsaI 82 CCATGG C^C[GA][CT]GG
649 CCATGG
EcoNI 449 CCTGGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1002 CCTCCTTTAGG
EcoO109I 476 GGGGCCC [GA]G^G[ACGT]CC[CT]
791 AGGTCCC
FokI 2104 CATCC GGATG(9/13)
1164 GGATG
HincII 1835 GTTGAC GT[CT]^[GA]AC
2111 GTTAAC
HindII 1835 GTTGAC GT[CT]^[GA]AC
2111 GTTAAC
MwoI 692 GCAGCAGGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
735 GCCAGCTGAGC
NcoI 82 CCATGG C^CATGG
649 CCATGG
SmaI 418 CCCGGG CCC^GGG
487 CCCGGG
TaqI 556 TCGA T^CGA
1362 TCGA
TthHB8I 556 TCGA T^CGA
1362 TCGA
XmaCI 418 CCCGGG C^CCGGG
487 CCCGGG
XmaI 418 CCCGGG C^CCGGG
487 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaI
AvaII BanI BanII BbvI BcnI
BfaI BmyI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstNI BstYI
Cac8I Cfr13I DdeI DpnII DrdI
EaeI EarI EcoRII EcoT14I Fnu4HI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI NciI NdeII
NlaIII NlaIV PleI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII