##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AccIII AgeI Alw44I AocI Aor51HI
ApaLI AscI AspI AvaI AviII
BamHI BbrPI BcgI BglI BseAI
BsiWI BsmBI BsoBI BspDI BspEI
BsrBI BssHII BstBI BstEII BstPI
Bsu36I ClaI CpoI Eco47III Eco81I
EcoO65I FseI FspI KspI MluI
MroI NaeI NgoMIV NheI NotI
NruI NspV PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PvuI PvuII RsrII SacII SalI
SapI SbfI SexAI SfiI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
Tth111I Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 195 GACGTC GACGT^C
AclI 6208 AACGTT AA^CGTT
AhdI 7997 GACTTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 3985 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 7997 GACTTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 409 CCTAGG C^CTAGG
BbeI 71 GGCGCC GGCGC^C
BbvCI 1725 CCTCAGC CCTCAGC(-5/-2)
BlnI 409 CCTAGG C^CTAGG
BlpI 5089 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 5089 GCTTAGC GC^T[ACGT]AGC
BsaWI 2503 TCCGGT [AT]^CCGG[AT]
BsiEI 110 CGGCCG CG[GA][CT]^CG
BsrFI 928 GCCGGT [GA]^CCGG[CT]
BssSI 2003 CTCGTG CACGAG(-5/-1)
BstAPI 2152 GCAGAAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 5089 GCTTAGC GC^T[ACGT]AGC
Cfr10I 928 GCCGGT [GA]^CCGG[CT]
DrdI 2707 GACCTCTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 110 CGGCCG C^GGCCG
Eam1105I 7997 GACTTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 110 CGGCCG C^GGCCG
Eco52I 110 CGGCCG C^GGCCG
EcoNI 569 CCTTTAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 464 GATATC GAT^ATC
HpaI 6649 GTTAAC GTT^AAC
KasI 71 GGCGCC G^GCGCC
NarI 71 GGCGCC GG^CGCC
PacI 4260 TTAATTAA TTAAT^TAA
Psp1406I 6208 AACGTT AA^CGTT
SacI 7449 GAGCTC GAGCT^C
ScaI 4952 AGTACT AGT^ACT
SfoI 71 GGCGCC GGC^GCC
SpeI 3944 ACTAGT A^CTAGT
SwaI 4494 ATTTAAAT ATTT^AAAT
XbaI 7610 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 2306 GGTACC G^GTACC
8410 GGTACC
AccII 32 CGCG CG^CG
104 CGCG
AcyI 71 GGCGCC G[GA]^CG[CT]C
195 GACGTC
ApaI 5447 GGGCCC GGGCC^C
6353 GGGCCC
Asp718I 2306 GGTACC G^GTACC
8410 GGTACC
BalI 603 TGGCCA TGG^CCA
2366 TGGCCA
BclI 1029 TGATCA T^GATCA
4290 TGATCA
BglII 5188 AGATCT A^GATCT
6596 AGATCT
BsaAI 1190 TACGTG [CT]AC^GT[GA]
2162 CACGTA
BsaBI 3997 GATAAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
6591 GATCAAGATC
BsaHI 71 GGCGCC G[GA]^CG[CT]C
195 GACGTC
Bsp1407I 2448 TGTACA T^GTACA
4169 TGTACA
BspMI 1672 GCAGGT ACCTGC(4/8)
5566 GCAGGT
BsrGI 2448 TGTACA T^GTACA
4169 TGTACA
Bst1107I 4840 GTATAC GTA^TAC
8195 GTATAC
BstUI 32 CGCG CG^CG
104 CGCG
BstZ17I 4840 GTATAC GTA^TAC
8195 GTATAC
DraIII 831 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
2530 CACTGGGTG
EcoT22I 5609 ATGCAT ATGCA^T
7728 ATGCAT
FauI 57 GCGGG CCCGC(4/6)
105 GCGGG
FbaI 1029 TGATCA T^GATCA
4290 TGATCA
Hin1I 71 GGCGCC G[GA]^CG[CT]C
195 GACGTC
HindIII 4219 AAGCTT A^AGCTT
4818 AAGCTT
KpnI 2306 GGTACC GGTAC^C
8410 GGTACC
MamI 3997 GATAAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
6591 GATCAAGATC
MfeI 1567 CAATTG C^AATTG
7814 CAATTG
MluNI 603 TGGCCA TGG^CCA
2366 TGGCCA
MscI 603 TGGCCA TGG^CCA
2366 TGGCCA
MspA1I 55 CAGCGG C[AC]G^C[GT]G
145 CCGCTG
MunI 1567 CAATTG C^AATTG
7814 CAATTG
MvnI 32 CGCG CG^CG
104 CGCG
NcoI 966 CCATGG C^CATGG
7004 CCATGG
NsiI 5609 ATGCAT ATGCA^T
7728 ATGCAT
SspBI 2448 TGTACA T^GTACA
4169 TGTACA
StuI 1040 AGGCCT AGG^CCT
1509 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AflIII AluI AlwI ApoI AseI
AsnI Asp700I AspHI AvaII BanI
BanII BbsI BbvI BciVI BcnI
BfaI BfrI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspHI BspLU11I BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NciI NdeI NdeII NlaIII
NlaIV NspI PleI PpuMI PshBI
PstI RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmnI