##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
Aor51HI ApaI AscI AspI AvaI
AviII AvrII BbrPI BciVI BfrI
BlnI BlpI Bpu1102I BsaBI BsaWI
BseAI BsiWI BsoBI BspDI BspEI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I CelII ClaI CpoI DrdI
Eco47III EcoO65I FseI FspI HgaI
HpaI KspI MamI MroI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacII SbfI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI SwaI
Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 4430 GGTACC G^GTACC
AccI 1072 GTCGAC GT^[AC][GT]AC
AhdI 4031 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 617 GTGCAC G^TGCAC
AocI 1209 CCTGAGG CC^T[ACGT]AGG
ApaLI 617 GTGCAC G^TGCAC
Asp718I 4430 GGTACC G^GTACC
AspEI 4031 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 5190 GGATCC G^GATCC
BcgI 1023 CGAGTGTTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 5673 GCCTCAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 5911 AGATCT A^GATCT
BsaAI 5902 TACGTG [CT]AC^GT[GA]
BsiEI 1177 CGGCCG CG[GA][CT]^CG
BspLU11I 5946 ACATGT A^CATGT
BsrBI 364 GAGCGG CCGCTC(-3/-3)
BsrFI 1033 GCCGGC [GA]^CCGG[CT]
BstAPI 6638 GCAGAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1209 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1033 GCCGGC [GA]^CCGG[CT]
DraIII 3431 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1177 CGGCCG C^GGCCG
Eam1105I 4031 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1177 CGGCCG C^GGCCG
Eco52I 1177 CGGCCG C^GGCCG
Eco81I 1209 CCTGAGG CC^T[ACGT]AGG
EcoNI 4168 CCTTTGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 6089 GAATTC G^AATTC
EcoRV 4877 GATATC GAT^ATC
EcoT22I 3569 ATGCAT ATGCA^T
FauI 6575 GCGGG CCCGC(4/6)
HindIII 1273 AAGCTT A^AGCTT
KpnI 4430 GGTACC GGTAC^C
MluI 1556 ACGCGT A^CGCGT
NaeI 1033 GCCGGC GCC^GGC
NdeI 3682 CATATG CA^TATG
NgoMIV 1033 GCCGGC G^CCGGC
NsiI 3569 ATGCAT ATGCA^T
PflMI 6305 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 814 AGGACCT [GA]G^G[AT]CC[CT]
SacI 1432 GAGCTC GAGCT^C
SalI 1072 GTCGAC G^TCGAC
SapI 974 GAAGAGC GCTCTTC(1/4)
SexAI 623 ACCAGGT A^CC[AT]GGT
Van91I 6305 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccII 180 CGCG CG^CG
1557 CGCG
AcyI 6292 GGCGCC G[GA]^CG[CT]C
6578 GGCGCC
AflIII 1556 ACGCGT A^C[GA][CT]GT
5946 ACATGT
BalI 2130 TGGCCA TGG^CCA
6516 TGGCCA
BbeI 6292 GGCGCC GGCGC^C
6578 GGCGCC
BsaHI 6292 GGCGCC G[GA]^CG[CT]C
6578 GGCGCC
BseRI 1914 GAGGAG GAGGAG(10/8)
4871 GAGGAG
BsmBI 1448 CGTCTC CGTCTC(1/5)
6084 GAGACG
BsmI 740 GAATGC GAATGC(1/-1)
4493 GAATGC
Bsp1407I 3130 TGTACA T^GTACA
4260 TGTACA
BspHI 533 TCATGA T^CATGA
860 TCATGA
BsrGI 3130 TGTACA T^GTACA
4260 TGTACA
BstUI 180 CGCG CG^CG
1557 CGCG
DraII 814 AGGACCT [GA]G^G[ACGT]CC[CT]
2676 AGGGCCT
DsaI 1067 CCATGG C^C[GA][CT]GG
5194 CCATGG
EcoO109I 814 AGGACCT [GA]G^G[ACGT]CC[CT]
2676 AGGGCCT
Hin1I 6292 GGCGCC G[GA]^CG[CT]C
6578 GGCGCC
KasI 6292 GGCGCC G^GCGCC
6578 GGCGCC
MfeI 5526 CAATTG C^AATTG
6117 CAATTG
MluNI 2130 TGGCCA TGG^CCA
6516 TGGCCA
MscI 2130 TGGCCA TGG^CCA
6516 TGGCCA
MspA1I 544 CAGCTG C[AC]G^C[GT]G
6306 CAGCTG
MunI 5526 CAATTG C^AATTG
6117 CAATTG
MvnI 180 CGCG CG^CG
1557 CGCG
NarI 6292 GGCGCC GG^CGCC
6578 GGCGCC
NcoI 1067 CCATGG C^CATGG
5194 CCATGG
NheI 3426 GCTAGC G^CTAGC
5791 GCTAGC
NspI 3877 ACATGC [GA]CATG^[CT]
5946 ACATGT
PvuII 544 CAGCTG CAG^CTG
6306 CAGCTG
RcaI 533 TCATGA T^CATGA
860 TCATGA
SfoI 6292 GGCGCC GGC^GCC
6578 GGCGCC
SspBI 3130 TGTACA T^GTACA
4260 TGTACA
SspI 4471 AATATT AAT^ATT
4591 AATATT
XbaI 4999 TCTAGA T^CTAGA
5108 TCTAGA
XcmI 1591 CCACCAAAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1654 CCAATGACGAAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI Asp700I
AspHI AvaII BanI BanII BbsI
BbvCI BbvI BclI BcnI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrDI BsrI
BssKI BssSI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI EaeI EarI EcoRII
EcoT14I FbaI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PshBI PstI
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI