##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AatII Acc65I AclI AgeI AscI
Asp718I AviII BamHI BbeI BbrPI
BsiWI Bsp1407I BspDI BspHI BsrGI
BstBI ClaI CpoI EcoNI FseI
FspI HpaI KasI KpnI MfeI
MluI MunI NarI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI Psp1406I PvuI RcaI
RsrII SalI SbfI SexAI SfoI
SfuI SgrAI SnaBI Sse8387I SspBI
SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccIII 871 TCCGGA T^CCGGA
AcyI 1190 GACGCC G[GA]^CG[CT]C
AflII 4538 CTTAAG C^TTAAG
AseI 4026 ATTAAT AT^TAAT
AsnI 4026 ATTAAT AT^TAAT
AvrII 1328 CCTAGG C^CTAGG
BcgI 5783 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 4538 CTTAAG C^TTAAG
BglII 443 AGATCT A^GATCT
BlnI 1328 CCTAGG C^CTAGG
BsaHI 1190 GACGCC G[GA]^CG[CT]C
BseAI 871 TCCGGA T^CCGGA
BspEI 871 TCCGGA T^CCGGA
BspLU11I 7108 ACATGT A^CATGT
BssHII 149 GCGCGC G^CGCGC
Bst1107I 2507 GTATAC GTA^TAC
BstEII 7230 GGTGACC G^GT[ACGT]ACC
BstPI 7230 GGTGACC G^GT[ACGT]ACC
BstZ17I 2507 GTATAC GTA^TAC
DraIII 3806 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 7230 GGTGACC G^GT[ACGT]ACC
EcoRV 6009 GATATC GAT^ATC
EcoT22I 7177 ATGCAT ATGCA^T
Hin1I 1190 GACGCC G[GA]^CG[CT]C
HindIII 3720 AAGCTT A^AGCTT
KspI 1337 CCGCGG CCGC^GG
MroI 871 TCCGGA T^CCGGA
NdeI 7295 CATATG CA^TATG
NsiI 7177 ATGCAT ATGCA^T
PshAI 981 GACGTGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4026 ATTAAT AT^TAAT
SacII 1337 CCGCGG CCGC^GG
SfiI 2435 GGCCAGGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 3295 ACTAGT A^CTAGT
XbaI 1715 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AlwNI 324 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
5546 CAGTTGCTG
AocI 3124 CCTCAGG CC^T[ACGT]AGG
5318 CCTGAGG
Asp700I 3367 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5674 GAACTTATTC
BbsI 1845 GAAGAC GAAGAC(2/6)
2085 GTCTTC
BlpI 5151 GCTCAGC GC^T[ACGT]AGC
5597 GCTAAGC
Bpu1102I 5151 GCTCAGC GC^T[ACGT]AGC
5597 GCTAAGC
BpuAI 1845 GAAGAC GAAGAC(2/6)
2085 GTCTTC
BsaBI 1064 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
6072 GATTATCATC
BsaWI 871 TCCGGA [AT]^CCGG[AT]
1375 ACCGGA
BsmI 2404 GAATGC GAATGC(1/-1)
3428 GCATTC
BsrFI 1534 GCCGGC [GA]^CCGG[CT]
2624 GCCGGC
BssSI 2031 CACGAG CACGAG(-5/-1)
3239 CACGAG
Bsu36I 3124 CCTCAGG CC^T[ACGT]AGG
5318 CCTGAGG
CelII 5151 GCTCAGC GC^T[ACGT]AGC
5597 GCTAAGC
Cfr10I 1534 GCCGGC [GA]^CCGG[CT]
2624 GCCGGC
Eco81I 3124 CCTCAGG CC^T[ACGT]AGG
5318 CCTGAGG
EcoRI 3599 GAATTC G^AATTC
4095 GAATTC
MamI 1064 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
6072 GATTATCATC
NaeI 1534 GCCGGC GCC^GGC
2624 GCCGGC
NgoMIV 1534 GCCGGC G^CCGGC
2624 GCCGGC
NheI 209 GCTAGC G^CTAGC
6150 GCTAGC
PstI 27 CTGCAG CTGCA^G
7096 CTGCAG
PvuII 187 CAGCTG CAG^CTG
1236 CAGCTG
SacI 271 GAGCTC GAGCT^C
4216 GAGCTC
SapI 1349 GCTCTTC GCTCTTC(1/4)
5562 GCTCTTC
SmaI 94 CCCGGG CCC^GGG
1420 CCCGGG
StuI 1198 AGGCCT AGG^CCT
2584 AGGCCT
XmaCI 94 CCCGGG C^CCGGG
1420 CCCGGG
XmaI 94 CCCGGG C^CCGGG
1420 CCCGGG
XmnI 3367 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5674 GAACTTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AhdI AluI Alw44I AlwI
Aor51HI ApaI ApaLI ApoI AspEI
AspHI AspI AvaI AvaII BalI
BanI BanII BbvCI BbvI BciVI
BclI BcnI BfaI BglI BmyI
BpmI BsaAI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflFI
PflMI PleI PpuMI RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI SphI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoII