##################################
# NO CUT ENZYMES ( 42 enzymes) #
##################################
AflII AgeI AscI AvrII BfrI
BlnI BlpI Bpu1102I BsiWI Bsp1407I
BsrGI BssHII Bst1107I BstZ17I CelII
CpoI FseI MfeI MluI MunI
NdeI NheI NotI PaeR7I PinAI
PmeI PpuMI PshAI PvuI RsrII
SalI SbfI ScaI SexAI SfiI
SgrAI SnaBI Sse8387I SspBI SwaI
XcmI XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 2406 GGTACC G^GTACC
AclI 282 AACGTT AA^CGTT
AocI 50 CCTCAGG CC^T[ACGT]AGG
Aor51HI 3212 AGCGCT AGC^GCT
ApaI 4690 GGGCCC GGGCC^C
AseI 1296 ATTAAT AT^TAAT
AsnI 1296 ATTAAT AT^TAAT
Asp718I 2406 GGTACC G^GTACC
AspI 4041 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 4489 TGCGCA TGC^GCA
BbrPI 5774 CACGTG CAC^GTG
BspDI 4786 ATCGAT AT^CGAT
BssSI 531 CTCGTG CACGAG(-5/-1)
BstBI 4982 TTCGAA TT^CGAA
Bsu36I 50 CCTCAGG CC^T[ACGT]AGG
ClaI 4786 ATCGAT AT^CGAT
DrdI 4170 GACTTGGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 4724 CGGCCG C^GGCCG
EclXI 4724 CGGCCG C^GGCCG
Eco47III 3212 AGCGCT AGC^GCT
Eco52I 4724 CGGCCG C^GGCCG
Eco81I 50 CCTCAGG CC^T[ACGT]AGG
FspI 4489 TGCGCA TGC^GCA
HincII 3009 GTTAAC GT[CT]^[GA]AC
HindII 3009 GTTAAC GT[CT]^[GA]AC
HpaI 3009 GTTAAC GTT^AAC
KpnI 2406 GGTACC GGTAC^C
KspI 4744 CCGCGG CCGC^GG
NruI 4406 TCGCGA TCG^CGA
NspV 4982 TTCGAA TT^CGAA
PacI 3222 TTAATTAA TTAAT^TAA
PflFI 4041 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 5774 CACGTG CAC^GTG
PmlI 5774 CACGTG CAC^GTG
PshBI 1296 ATTAAT AT^TAAT
Psp1406I 282 AACGTT AA^CGTT
SacII 4744 CCGCGG CCGC^GG
SfuI 4982 TTCGAA TT^CGAA
SpeI 4938 ACTAGT A^CTAGT
SphI 2217 GCATGC GCATG^C
Tth111I 4041 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AatII 774 GACGTC GACGT^C
3876 GACGTC
AccI 1242 GTCTAC GT^[AC][GT]AC
4953 GTAGAC
AhdI 2674 GACAGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4395 GACCAGAAGTC
Alw44I 2187 GTGCAC G^TGCAC
4443 GTGCAC
ApaLI 2187 GTGCAC G^TGCAC
4443 GTGCAC
AspEI 2674 GACAGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4395 GACCAGAAGTC
BalI 3760 TGGCCA TGG^CCA
4435 TGGCCA
BbeI 45 GGCGCC GGCGC^C
4005 GGCGCC
BbvCI 2579 CCTCAGC CCTCAGC(-5/-2)
4643 GCTGAGG
BcgI 713 CGAAGAGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
440 GCAGGCCAATCG
BciVI 5806 GGATAC GTATCC(6/5)
6079 GGATAC
BglII 2743 AGATCT A^GATCT
5579 AGATCT
BsgI 819 GTGCAG GTGCAG(16/14)
1018 GTGCAG
BsiEI 2909 CGACCG CG[GA][CT]^CG
4724 CGGCCG
BspLU11I 251 ACATGT A^CATGT
3341 ACATGT
BsrDI 1192 CATTGC GCAATG(2/0)
3773 GCAATG
BsrFI 1207 GCCGGC [GA]^CCGG[CT]
4726 GCCGGC
BstEII 368 GGTGACC G^GT[ACGT]ACC
4163 GGTAACC
BstPI 368 GGTGACC G^GT[ACGT]ACC
4163 GGTAACC
Cfr10I 1207 GCCGGC [GA]^CCGG[CT]
4726 GCCGGC
DraII 643 GGGGCCT [GA]G^G[ACGT]CC[CT]
4348 AGGGCCT
DraIII 1346 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
1408 CACTTAGTG
Eam1105I 2674 GACAGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4395 GACCAGAAGTC
EcoNI 50 CCTCAGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3909 CCTGACCCAGG
EcoO109I 643 GGGGCCT [GA]G^G[ACGT]CC[CT]
4348 AGGGCCT
EcoO65I 368 GGTGACC G^GT[ACGT]ACC
4163 GGTAACC
EcoT22I 2709 ATGCAT ATGCA^T
3107 ATGCAT
KasI 45 GGCGCC G^GCGCC
4005 GGCGCC
MluNI 3760 TGGCCA TGG^CCA
4435 TGGCCA
MscI 3760 TGGCCA TGG^CCA
4435 TGGCCA
NaeI 1207 GCCGGC GCC^GGC
4726 GCCGGC
NarI 45 GGCGCC GG^CGCC
4005 GGCGCC
NgoMIV 1207 GCCGGC G^CCGGC
4726 GCCGGC
NsiI 2709 ATGCAT ATGCA^T
3107 ATGCAT
SacI 528 GAGCTC GAGCT^C
1637 GAGCTC
SapI 1869 GAAGAGC GCTCTTC(1/4)
2515 GCTCTTC
SfoI 45 GGCGCC GGC^GCC
4005 GGCGCC
SmaI 680 CCCGGG CCC^GGG
4146 CCCGGG
XbaI 563 TCTAGA T^CTAGA
3884 TCTAGA
XmaCI 680 CCCGGG C^CCGGG
4146 CCCGGG
XmaI 680 CCCGGG C^CCGGG
4146 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccII AccIII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApoI Asp700I AspHI AvaI AvaII
BamHI BanI BanII BbsI BbvI
BclI BcnI BfaI BglI BmyI
BpmI BpuAI BsaAI BsaBI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DsaI EaeI EarI
EcoRI EcoRII EcoRV EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MnlI MroI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmnI