##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
AatII Acc65I AclI AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspI BbrPI BcgI BsaAI
BsiWI BspDI BspHI BssHII ClaI
CpoI Eco47III EcoRV FseI HindIII
KpnI MluI PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SbfI SfiI SmaI SnaBI Sse8387I
SspI Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AhdI 5703 GACCTAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 6227 GGGCCC GGGCC^C
AspEI 5703 GACCTAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI 111 CCTCAGC CCTCAGC(-5/-2)
BciVI 1358 GTATCC GTATCC(6/5)
BsmBI 2658 GAGACG CGTCTC(1/5)
BsrBI 2264 CCGCTC CCGCTC(-3/-3)
BssSI 531 CTCGTG CACGAG(-5/-1)
Bst1107I 2571 GTATAC GTA^TAC
BstAPI 3044 GCAGGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2499 TTCGAA TT^CGAA
BstXI 2158 CCAACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2571 GTATAC GTA^TAC
DrdI 6397 GACTAGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 5703 GACCTAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 950 GAATTC G^AATTC
EcoT22I 4796 ATGCAT ATGCA^T
MfeI 2786 CAATTG C^AATTG
MunI 2786 CAATTG C^AATTG
NcoI 1787 CCATGG C^CATGG
NheI 6290 GCTAGC G^CTAGC
NruI 70 TCGCGA TCG^CGA
NsiI 4796 ATGCAT ATGCA^T
NspV 2499 TTCGAA TT^CGAA
SexAI 1648 ACCTGGT A^CC[AT]GGT
SfuI 2499 TTCGAA TT^CGAA
SgrAI 2590 CGCCGGCG C[GA]^CCGG[CT]G
SpeI 4817 ACTAGT A^CTAGT
SphI 5044 GCATGC GCATG^C
SwaI 6693 ATTTAAAT ATTT^AAAT
XbaI 4445 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccI 2571 GTATAC GT^[AC][GT]AC
4776 GTAGAC
AccIII 2848 TCCGGA T^CCGGA
3195 TCCGGA
AflII 3585 CTTAAG C^TTAAG
4185 CTTAAG
Asp700I 2460 GAATACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4895 GAACTGATTC
AviII 2416 TGCGCA TGC^GCA
5883 TGCGCA
BamHI 1582 GGATCC G^GATCC
3425 GGATCC
BbeI 152 GGCGCC GGCGC^C
3381 GGCGCC
BbsI 703 GAAGAC GAAGAC(2/6)
4176 GTCTTC
BclI 4055 TGATCA T^GATCA
4747 TGATCA
BfrI 3585 CTTAAG C^TTAAG
4185 CTTAAG
BglI 2263 GCCGCTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2440 GCCTAAACGGC
BpuAI 703 GAAGAC GAAGAC(2/6)
4176 GTCTTC
BsaBI 1406 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5331 GATCCAAATC
BseAI 2848 TCCGGA T^CCGGA
3195 TCCGGA
BspEI 2848 TCCGGA T^CCGGA
3195 TCCGGA
BspLU11I 1514 ACATGT A^CATGT
6166 ACATGT
DraIII 906 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
2723 CACAGAGTG
EagI 339 CGGCCG C^GGCCG
3026 CGGCCG
EclXI 339 CGGCCG C^GGCCG
3026 CGGCCG
Eco52I 339 CGGCCG C^GGCCG
3026 CGGCCG
EcoNI 4394 CCTGGACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5204 CCTATTAAAGG
FbaI 4055 TGATCA T^GATCA
4747 TGATCA
FspI 2416 TGCGCA TGC^GCA
5883 TGCGCA
KasI 152 GGCGCC G^GCGCC
3381 GGCGCC
MamI 1406 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5331 GATCCAAATC
MroI 2848 TCCGGA T^CCGGA
3195 TCCGGA
NarI 152 GGCGCC GG^CGCC
3381 GGCGCC
NdeI 4751 CATATG CA^TATG
4983 CATATG
NotI 338 GCGGCCGC GC^GGCCGC
3025 GCGGCCGC
PaeR7I 262 CTCGAG C^TCGAG
2805 CTCGAG
PflMI 1512 CCACATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6568 CCATTCACTGG
SapI 2678 GAAGAGC GCTCTTC(1/4)
2123 GCTCTTC
ScaI 1537 AGTACT AGT^ACT
4921 AGTACT
SfoI 152 GGCGCC GGC^GCC
3381 GGCGCC
StuI 2180 AGGCCT AGG^CCT
3491 AGGCCT
Van91I 1512 CCACATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6568 CCATTCACTGG
XhoI 262 CTCGAG C^TCGAG
2805 CTCGAG
XmnI 2460 GAATACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4895 GAACTGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI AspHI AvaI AvaII AvrII
BalI BanI BanII BbvI BcnI
BfaI BglII BlnI BlpI BmyI
BpmI Bpu1102I BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspMI BsrDI
BsrFI BsrGI BsrI BssKI BstEII
BstF5I BstNI BstPI BstUI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaI HpaII HphI ItaI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
SacI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII