##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII AccI AccIII AclI AgeI
AocI Aor51HI AscI AspI AvaI
AviII AvrII BamHI BbrPI BbvCI
BcgI BciVI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BseAI BsiWI
BsmBI BsoBI BspDI BspEI BspMI
BsrFI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraIII DrdI Eco47III
Eco81I EcoNI EcoO65I EcoT22I FseI
FspI HincII HindII HpaI MamI
MfeI MluI MroI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SalI
SbfI SexAI SfiI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SspI
SwaI Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 2071 GGTACC G^GTACC
AcyI 1420 GGCGCC G[GA]^CG[CT]C
AflII 2116 CTTAAG C^TTAAG
AflIII 1567 ACATGT A^C[GA][CT]GT
AhdI 1491 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 561 GTGCAC G^TGCAC
ApaI 1396 GGGCCC GGGCC^C
ApaLI 561 GTGCAC G^TGCAC
AseI 63 ATTAAT AT^TAAT
AsnI 63 ATTAAT AT^TAAT
Asp718I 2071 GGTACC G^GTACC
AspEI 1491 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 1420 GGCGCC GGCGC^C
BclI 865 TGATCA T^GATCA
BfrI 2116 CTTAAG C^TTAAG
BsaHI 1420 GGCGCC G[GA]^CG[CT]C
BsaWI 431 ACCGGA [AT]^CCGG[AT]
Bsp1407I 1663 TGTACA T^GTACA
BspLU11I 1567 ACATGT A^CATGT
BsrBI 2018 GAGCGG CCGCTC(-3/-3)
BsrGI 1663 TGTACA T^GTACA
BstAPI 1097 GCAGGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 550 CCAACCCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Eam1105I 1491 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1368 GATATC GAT^ATC
FbaI 865 TGATCA T^GATCA
Hin1I 1420 GGCGCC G[GA]^CG[CT]C
KasI 1420 GGCGCC G^GCGCC
KpnI 2071 GGTACC GGTAC^C
KspI 29 CCGCGG CCGC^GG
MslI 1319 CACAAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 1420 GGCGCC GG^CGCC
NspI 1567 ACATGT [GA]CATG^[CT]
PpuMI 1483 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 63 ATTAAT AT^TAAT
PvuII 732 CAGCTG CAG^CTG
SacI 1657 GAGCTC GAGCT^C
SacII 29 CCGCGG CCGC^GG
SapI 1803 GAAGAGC GCTCTTC(1/4)
SfoI 1420 GGCGCC GGC^GCC
SpeI 125 ACTAGT A^CTAGT
SspBI 1663 TGTACA T^GTACA
TaqI 310 TCGA T^CGA
TthHB8I 310 TCGA T^CGA
XbaI 839 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 20 CGCG CG^CG
30 CGCG
AvaII 1484 GGACC G^G[AT]CC
1862 GGACC
BalI 1258 TGGCCA TGG^CCA
1619 TGGCCA
BcnI 6 CCGGG CC^[GC]GG
2052 CCCGG
BglI 984 GCCTCCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1575 GCCAAGGTGGC
BsgI 1224 CTGCAC GTGCAG(16/14)
2010 GTGCAG
BsiEI 3 CGGCCG CG[GA][CT]^CG
32 CGGCCG
BsmI 186 GAATGC GAATGC(1/-1)
2174 GCATTC
BstUI 20 CGCG CG^CG
30 CGCG
DraI 573 TTTAAA TTT^AAA
2156 TTTAAA
DsaI 29 CCGCGG C^C[GA][CT]GG
1948 CCACGG
EagI 3 CGGCCG C^GGCCG
32 CGGCCG
EclXI 3 CGGCCG C^GGCCG
32 CGGCCG
Eco52I 3 CGGCCG C^GGCCG
32 CGGCCG
EcoRI 375 GAATTC G^AATTC
1776 GAATTC
HaeII 1111 GGCGCT [GA]GCGC^[CT]
1420 GGCGCC
HgaI 889 GCGTC GACGC(5/10)
1281 GCGTC
MaeII 749 ACGT A^CGT
1240 ACGT
MluNI 1258 TGGCCA TGG^CCA
1619 TGGCCA
MscI 1258 TGGCCA TGG^CCA
1619 TGGCCA
MvnI 20 CGCG CG^CG
30 CGCG
NciI 6 CCGGG CC^[GC]GG
2052 CCCGG
PflMI 365 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
728 CCAGCAGCTGG
ScaI 415 AGTACT AGT^ACT
1516 AGTACT
SmlI 500 CTTGAG C^T[CT][GA]AG
2116 CTTAAG
StuI 954 AGGCCT AGG^CCT
1586 AGGCCT
Van91I 365 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
728 CCAGCAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI BanI
BanII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspHI BsrDI BsrI BssKI
BssSI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV PleI PstI RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XcmI XhoII
XmnI