##################################
# NO CUT ENZYMES ( 42 enzymes) #
##################################
Acc65I AccIII AclI AgeI Aor51HI
AscI Asp718I BamHI BbrPI BseAI
BsiWI BspDI BspEI BssHII BstAPI
BstBI ClaI CpoI Eco47III FseI
KpnI MfeI MluI MroI MunI
NotI NruI NspV PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SalI SexAI SfuI SgrAI SnaBI
StuI SwaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 757 GACGTC GACGT^C
Alw44I 4945 GTGCAC G^TGCAC
AlwNI 5429 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1754 CCTGAGG CC^T[ACGT]AGG
ApaLI 4945 GTGCAC G^TGCAC
AspI 3980 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 523 TGCGCA TGC^GCA
AvrII 5050 CCTAGG C^CTAGG
BclI 5856 TGATCA T^GATCA
BlnI 5050 CCTAGG C^CTAGG
BsaBI 614 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 199 ACCGGA [AT]^CCGG[AT]
BsmBI 755 GAGACG CGTCTC(1/5)
BsmI 4686 GAATGC GAATGC(1/-1)
Bsp1407I 2374 TGTACA T^GTACA
BsrGI 2374 TGTACA T^GTACA
Bsu36I 1754 CCTGAGG CC^T[ACGT]AGG
DraIII 660 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 425 GACTAACGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1754 CCTGAGG CC^T[ACGT]AGG
EcoRV 6137 GATATC GAT^ATC
EcoT22I 2076 ATGCAT ATGCA^T
FbaI 5856 TGATCA T^GATCA
FspI 523 TGCGCA TGC^GCA
HgaI 134 GCGTC GACGC(5/10)
KspI 653 CCGCGG CCGC^GG
MamI 614 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 2595 CATATG CA^TATG
NheI 2128 GCTAGC G^CTAGC
NsiI 2076 ATGCAT ATGCA^T
PacI 1339 TTAATTAA TTAAT^TAA
PaeR7I 675 CTCGAG C^TCGAG
PflFI 3980 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 5695 CCAGCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 5430 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 4144 GACATGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 653 CCGCGG CCGC^GG
SbfI 1022 CCTGCAGG CCTGCA^GG
SmaI 150 CCCGGG CCC^GGG
SpeI 3063 ACTAGT A^CTAGT
SphI 3931 GCATGC GCATG^C
Sse8387I 1022 CCTGCAGG CCTGCA^GG
SspBI 2374 TGTACA T^GTACA
Tth111I 3980 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 5695 CCAGCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1796 TCTAGA T^CTAGA
XcmI 481 CCAACCGGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 675 CTCGAG C^TCGAG
XmaCI 150 CCCGGG C^CCGGG
XmaI 150 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AflII 2536 CTTAAG C^TTAAG
6830 CTTAAG
AhdI 1142 GACGGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6065 GACAGGATGTC
AspEI 1142 GACGGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6065 GACAGGATGTC
BbsI 5561 GAAGAC GAAGAC(2/6)
5322 GTCTTC
BcgI 866 GCATGAGTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
970 GCAGCCTGATCG
BfrI 2536 CTTAAG C^TTAAG
6830 CTTAAG
BglII 4279 AGATCT A^GATCT
4663 AGATCT
BlpI 981 GCTCAGC GC^T[ACGT]AGC
6271 GCTCAGC
Bpu1102I 981 GCTCAGC GC^T[ACGT]AGC
6271 GCTCAGC
BpuAI 5561 GAAGAC GAAGAC(2/6)
5322 GTCTTC
BsaAI 5227 TACGTG [CT]AC^GT[GA]
6964 TACGTG
BspLU11I 2138 ACATGT A^CATGT
2882 ACATGT
BssSI 781 CACGAG CACGAG(-5/-1)
1894 CACGAG
Bst1107I 3544 GTATAC GTA^TAC
6728 GTATAC
BstEII 3625 GGTTACC G^GT[ACGT]ACC
5163 GGTAACC
BstPI 3625 GGTTACC G^GT[ACGT]ACC
5163 GGTAACC
BstZ17I 3544 GTATAC GTA^TAC
6728 GTATAC
CelII 981 GCTCAGC GC^T[ACGT]AGC
6271 GCTCAGC
EagI 56 CGGCCG C^GGCCG
534 CGGCCG
Eam1105I 1142 GACGGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6065 GACAGGATGTC
EclXI 56 CGGCCG C^GGCCG
534 CGGCCG
Eco52I 56 CGGCCG C^GGCCG
534 CGGCCG
EcoO65I 3625 GGTTACC G^GT[ACGT]ACC
5163 GGTAACC
HincII 1386 GTTAAC GT[CT]^[GA]AC
2936 GTTAAC
HindII 1386 GTTAAC GT[CT]^[GA]AC
2936 GTTAAC
HindIII 1865 AAGCTT A^AGCTT
2564 AAGCTT
HpaI 1386 GTTAAC GTT^AAC
2936 GTTAAC
NaeI 54 GCCGGC GCC^GGC
273 GCCGGC
NgoMIV 54 GCCGGC G^CCGGC
273 GCCGGC
SapI 3495 GAAGAGC GCTCTTC(1/4)
2236 GCTCTTC
ScaI 343 AGTACT AGT^ACT
3438 AGTACT
SfiI 535 GGCCGTCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
5799 GGCCTGTCCGGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI ApaI
ApoI AseI AsnI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbeI BbvCI BbvI BciVI BcnI
BfaI BglI BmyI BpmI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PshBI
PstI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI