################################## # NO CUT ENZYMES ( 42 enzymes) # ################################## Acc65I AccIII AclI AgeI Aor51HI AscI Asp718I BamHI BbrPI BseAI BsiWI BspDI BspEI BssHII BstAPI BstBI ClaI CpoI Eco47III FseI KpnI MfeI MluI MroI MunI NotI NruI NspV PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SalI SexAI SfuI SgrAI SnaBI StuI SwaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AatII 757 GACGTC GACGT^C Alw44I 4945 GTGCAC G^TGCAC AlwNI 5429 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1754 CCTGAGG CC^T[ACGT]AGG ApaLI 4945 GTGCAC G^TGCAC AspI 3980 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 523 TGCGCA TGC^GCA AvrII 5050 CCTAGG C^CTAGG BclI 5856 TGATCA T^GATCA BlnI 5050 CCTAGG C^CTAGG BsaBI 614 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 199 ACCGGA [AT]^CCGG[AT] BsmBI 755 GAGACG CGTCTC(1/5) BsmI 4686 GAATGC GAATGC(1/-1) Bsp1407I 2374 TGTACA T^GTACA BsrGI 2374 TGTACA T^GTACA Bsu36I 1754 CCTGAGG CC^T[ACGT]AGG DraIII 660 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 425 GACTAACGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1754 CCTGAGG CC^T[ACGT]AGG EcoRV 6137 GATATC GAT^ATC EcoT22I 2076 ATGCAT ATGCA^T FbaI 5856 TGATCA T^GATCA FspI 523 TGCGCA TGC^GCA HgaI 134 GCGTC GACGC(5/10) KspI 653 CCGCGG CCGC^GG MamI 614 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NdeI 2595 CATATG CA^TATG NheI 2128 GCTAGC G^CTAGC NsiI 2076 ATGCAT ATGCA^T PacI 1339 TTAATTAA TTAAT^TAA PaeR7I 675 CTCGAG C^TCGAG PflFI 3980 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 5695 CCAGCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 5430 AGGACCT [GA]G^G[AT]CC[CT] PshAI 4144 GACATGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 653 CCGCGG CCGC^GG SbfI 1022 CCTGCAGG CCTGCA^GG SmaI 150 CCCGGG CCC^GGG SpeI 3063 ACTAGT A^CTAGT SphI 3931 GCATGC GCATG^C Sse8387I 1022 CCTGCAGG CCTGCA^GG SspBI 2374 TGTACA T^GTACA Tth111I 3980 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 5695 CCAGCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1796 TCTAGA T^CTAGA XcmI 481 CCAACCGGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 675 CTCGAG C^TCGAG XmaCI 150 CCCGGG C^CCGGG XmaI 150 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AflII 2536 CTTAAG C^TTAAG 6830 CTTAAG AhdI 1142 GACGGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 6065 GACAGGATGTC AspEI 1142 GACGGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 6065 GACAGGATGTC BbsI 5561 GAAGAC GAAGAC(2/6) 5322 GTCTTC BcgI 866 GCATGAGTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 970 GCAGCCTGATCG BfrI 2536 CTTAAG C^TTAAG 6830 CTTAAG BglII 4279 AGATCT A^GATCT 4663 AGATCT BlpI 981 GCTCAGC GC^T[ACGT]AGC 6271 GCTCAGC Bpu1102I 981 GCTCAGC GC^T[ACGT]AGC 6271 GCTCAGC BpuAI 5561 GAAGAC GAAGAC(2/6) 5322 GTCTTC BsaAI 5227 TACGTG [CT]AC^GT[GA] 6964 TACGTG BspLU11I 2138 ACATGT A^CATGT 2882 ACATGT BssSI 781 CACGAG CACGAG(-5/-1) 1894 CACGAG Bst1107I 3544 GTATAC GTA^TAC 6728 GTATAC BstEII 3625 GGTTACC G^GT[ACGT]ACC 5163 GGTAACC BstPI 3625 GGTTACC G^GT[ACGT]ACC 5163 GGTAACC BstZ17I 3544 GTATAC GTA^TAC 6728 GTATAC CelII 981 GCTCAGC GC^T[ACGT]AGC 6271 GCTCAGC EagI 56 CGGCCG C^GGCCG 534 CGGCCG Eam1105I 1142 GACGGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 6065 GACAGGATGTC EclXI 56 CGGCCG C^GGCCG 534 CGGCCG Eco52I 56 CGGCCG C^GGCCG 534 CGGCCG EcoO65I 3625 GGTTACC G^GT[ACGT]ACC 5163 GGTAACC HincII 1386 GTTAAC GT[CT]^[GA]AC 2936 GTTAAC HindII 1386 GTTAAC GT[CT]^[GA]AC 2936 GTTAAC HindIII 1865 AAGCTT A^AGCTT 2564 AAGCTT HpaI 1386 GTTAAC GTT^AAC 2936 GTTAAC NaeI 54 GCCGGC GCC^GGC 273 GCCGGC NgoMIV 54 GCCGGC G^CCGGC 273 GCCGGC SapI 3495 GAAGAGC GCTCTTC(1/4) 2236 GCTCTTC ScaI 343 AGTACT AGT^ACT 3438 AGTACT SfiI 535 GGCCGTCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 5799 GGCCTGTCCGGCC ######################################### # 3 OR MORE CUT ENZYMES ( 134 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AflIII AluI AlwI ApaI ApoI AseI AsnI Asp700I AspHI AvaI AvaII BalI BanI BanII BbeI BbvCI BbvI BciVI BcnI BfaI BglI BmyI BpmI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoNI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV NspI PleI PshBI PstI PvuII RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI