##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AccIII AflII Aor51HI AscI AviII
BamHI BcgI BfrI BlpI Bpu1102I
BseAI BsiEI BsiWI BspDI BspEI
BssHII BstEII BstPI CelII ClaI
CpoI DrdI EagI EclXI Eco47III
Eco52I EcoO65I EcoRV FseI FspI
HincII HindII HindIII HpaI KspI
MluI MroI NaeI NdeI NgoMIV
NotI NruI PacI PaeR7I PmeI
PshAI PvuI RsrII SacII SalI
SbfI SfiI SgrAI SnaBI SpeI
Sse8387I StuI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 5517 GACGTC GACGT^C
AclI 6294 AACGTT AA^CGTT
AgeI 2600 ACCGGT A^CCGGT
ApaI 6090 GGGCCC GGGCC^C
AvrII 958 CCTAGG C^CTAGG
BbeI 4066 GGCGCC GGCGC^C
BbrPI 6013 CACGTG CAC^GTG
BciVI 1468 GGATAC GTATCC(6/5)
BclI 5749 TGATCA T^GATCA
BglI 6083 GCCACAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 958 CCTAGG C^CTAGG
BsaAI 6013 CACGTG [CT]AC^GT[GA]
BsmI 632 GAATGC GAATGC(1/-1)
BsrBI 4979 CCGCTC CCGCTC(-3/-3)
BsrFI 2600 ACCGGT [GA]^CCGG[CT]
BstAPI 4001 GCAAGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3796 TTCGAA TT^CGAA
Cfr10I 2600 ACCGGT [GA]^CCGG[CT]
DraIII 167 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT22I 5389 ATGCAT ATGCA^T
FbaI 5749 TGATCA T^GATCA
HaeII 4066 GGCGCC [GA]GCGC^[CT]
KasI 4066 GGCGCC G^GCGCC
MfeI 1982 CAATTG C^AATTG
MunI 1982 CAATTG C^AATTG
NarI 4066 GGCGCC GG^CGCC
NheI 390 GCTAGC G^CTAGC
NsiI 5389 ATGCAT ATGCA^T
NspV 3796 TTCGAA TT^CGAA
PinAI 2600 ACCGGT A^CCGGT
PmaCI 6013 CACGTG CAC^GTG
PmlI 6013 CACGTG CAC^GTG
Psp1406I 6294 AACGTT AA^CGTT
PvuII 5476 CAGCTG CAG^CTG
SacI 2500 GAGCTC GAGCT^C
SapI 5720 GAAGAGC GCTCTTC(1/4)
ScaI 1385 AGTACT AGT^ACT
SexAI 5887 ACCAGGT A^CC[AT]GGT
SfoI 4066 GGCGCC GGC^GCC
SfuI 3796 TTCGAA TT^CGAA
SphI 4594 GCATGC GCATG^C
SwaI 3286 ATTTAAAT ATTT^AAAT
XcmI 2566 CCAGTCAGATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Acc65I 129 GGTACC G^GTACC
1226 GGTACC
AflIII 1353 ACATGT A^C[GA][CT]GT
4588 ACATGT
AhdI 294 GACACATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
923 GACTGAATGTC
Asp700I 1922 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6293 GAACGTTTTC
Asp718I 129 GGTACC G^GTACC
1226 GGTACC
AspEI 294 GACACATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
923 GACTGAATGTC
BglII 556 AGATCT A^GATCT
4822 AGATCT
BsaWI 2600 ACCGGT [AT]^CCGG[AT]
6380 TCCGGT
BspHI 5872 TCATGA T^CATGA
6301 TCATGA
BspLU11I 1353 ACATGT A^CATGT
4588 ACATGT
BspMI 5038 ACCTGC ACCTGC(4/8)
5404 ACCTGC
Bst1107I 742 GTATAC GTA^TAC
1460 GTATAC
BstZ17I 742 GTATAC GTA^TAC
1460 GTATAC
DsaI 5290 CCATGG C^C[GA][CT]GG
6112 CCATGG
Eam1105I 294 GACACATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
923 GACTGAATGTC
EcoRI 590 GAATTC G^AATTC
5830 GAATTC
KpnI 129 GGTACC GGTAC^C
1226 GGTACC
NcoI 5290 CCATGG C^CATGG
6112 CCATGG
PpuMI 1337 AGGACCT [GA]G^G[AT]CC[CT]
5401 GGGACCT
PstI 1995 CTGCAG CTGCA^G
3817 CTGCAG
RcaI 5872 TCATGA T^CATGA
6301 TCATGA
XbaI 2798 TCTAGA T^CTAGA
6443 TCTAGA
XmnI 1922 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6293 GAACGTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI Alw44I AlwI AlwNI
AocI ApaLI ApoI AseI AsnI
AspHI AspI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaBI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I Bsp1407I
BsrDI BsrGI BsrI BssKI BssSI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflFI PflMI PleI PshBI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XhoII XmaCI XmaI