##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII Acc65I AgeI AocI ApaI
AscI Asp718I BamHI BbeI BbrPI
BcgI BlpI Bpu1102I BsgI BsiWI
BsmBI BspDI BsrBI BsrFI BssHII
BstAPI BstBI BstEII BstPI Bsu36I
CelII Cfr10I ClaI CpoI Eco81I
EcoO65I EcoRV FseI KasI KpnI
MluI NaeI NarI NcoI NgoMIV
NheI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PvuI RsrII SacI SalI SbfI
SfiI SfoI SfuI SgrAI SnaBI
Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AccII 341 CGCG CG^CG
AccIII 1349 TCCGGA T^CCGGA
AcyI 914 GACGCC G[GA]^CG[CT]C
AhdI 1580 GACTGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 6125 GTGCAC G^TGCAC
AlwNI 4663 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1570 AGCGCT AGC^GCT
ApaLI 6125 GTGCAC G^TGCAC
AspEI 1580 GACTGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2989 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 18 CCCGGG C^[CT]CG[GA]G
AviII 2717 TGCGCA TGC^GCA
AvrII 4703 CCTAGG C^CTAGG
BanI 1138 GGTGCC G^G[CT][GA]CC
BbvCI 457 CCTCAGC CCTCAGC(-5/-2)
BclI 3776 TGATCA T^GATCA
BglI 201 GCCAATGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 4703 CCTAGG C^CTAGG
BsaHI 914 GACGCC G[GA]^CG[CT]C
BsaI 4027 GAGACC GGTCTC(1/5)
BsaWI 1349 TCCGGA [AT]^CCGG[AT]
BseAI 1349 TCCGGA T^CCGGA
BsiEI 445 CGGCCG CG[GA][CT]^CG
BsoBI 18 CCCGGG C^[CT]CG[GA]G
BspEI 1349 TCCGGA T^CCGGA
BspLU11I 6129 ACATGT A^CATGT
BsrDI 1658 GCAATG GCAATG(2/0)
BssSI 6122 CTCGTG CACGAG(-5/-1)
BstUI 341 CGCG CG^CG
DraIII 3571 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2279 GACATGCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 445 CGGCCG C^GGCCG
Eam1105I 1580 GACTGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 445 CGGCCG C^GGCCG
Eco47III 1570 AGCGCT AGC^GCT
Eco52I 445 CGGCCG C^GGCCG
EcoNI 1106 CCTGCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 3776 TGATCA T^GATCA
FspI 2717 TGCGCA TGC^GCA
HgaI 914 GACGC GACGC(5/10)
Hin1I 914 GACGCC G[GA]^CG[CT]C
KspI 340 CCGCGG CCGC^GG
MroI 1349 TCCGGA T^CCGGA
MvnI 341 CGCG CG^CG
NdeI 1334 CATATG CA^TATG
NotI 444 GCGGCCGC GC^GGCCGC
PflFI 2989 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 840 CTGCAG CTGCA^G
SacII 340 CCGCGG CCGC^GG
ScaI 6056 AGTACT AGT^ACT
SexAI 5212 ACCAGGT A^CC[AT]GGT
SmaI 18 CCCGGG CCC^GGG
SpeI 6443 ACTAGT A^CTAGT
SphI 3923 GCATGC GCATG^C
StuI 41 AGGCCT AGG^CCT
Tth111I 2989 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1172 TCTAGA T^CTAGA
XmaCI 18 CCCGGG C^CCGGG
XmaI 18 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AclI 800 AACGTT AA^CGTT
2381 AACGTT
AflIII 2031 ACACGT A^C[GA][CT]GT
6129 ACATGT
AspHI 4671 GTGCTC G[AT]GC[AT]^C
6125 GTGCAC
BglII 2676 AGATCT A^GATCT
4428 AGATCT
BsaAI 988 TACGTG [CT]AC^GT[GA]
2032 CACGTA
BsaBI 1164 GATAATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1620 GATGGTAATC
BsiHKAI 4671 GTGCTC G[AT]GC[AT]^C
6125 GTGCAC
Bsp1407I 1487 TGTACA T^GTACA
4610 TGTACA
BspMI 1360 ACCTGC ACCTGC(4/8)
3298 GCAGGT
BsrGI 1487 TGTACA T^GTACA
4610 TGTACA
EcoRI 1717 GAATTC G^AATTC
3291 GAATTC
EcoT14I 505 CCTTGG C^C[AT][AT]GG
4703 CCTAGG
EcoT22I 3925 ATGCAT ATGCA^T
4968 ATGCAT
HaeII 1570 AGCGCT [GA]GCGC^[CT]
2085 AGCGCC
HincII 2833 GTTAAC GT[CT]^[GA]AC
6447 GTTAAC
HindII 2833 GTTAAC GT[CT]^[GA]AC
6447 GTTAAC
HpaI 2833 GTTAAC GTT^AAC
6447 GTTAAC
MamI 1164 GATAATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1620 GATGGTAATC
MfeI 2008 CAATTG C^AATTG
5333 CAATTG
MunI 2008 CAATTG C^AATTG
5333 CAATTG
NsiI 3925 ATGCAT ATGCA^T
4968 ATGCAT
PflMI 170 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3669 CCAGCTGTTGG
Psp1406I 800 AACGTT AA^CGTT
2381 AACGTT
SapI 2517 GCTCTTC GCTCTTC(1/4)
2967 GCTCTTC
SspBI 1487 TGTACA T^GTACA
4610 TGTACA
StyI 505 CCTTGG C^C[AT][AT]GG
4703 CCTAGG
TaqI 1735 TCGA T^CGA
4675 TCGA
TthHB8I 1735 TCGA T^CGA
4675 TCGA
Van91I 170 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3669 CCAGCTGTTGG
XcmI 511 CCACCCCAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2981 CCACATGAGACATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AluI AlwI ApoI AseI AsnI
Asp700I AvaII BalI BanII BbsI
BbvI BciVI BcnI BfaI BfrI
BmyI BpmI BpuAI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspHI BsrI BssKI Bst1107I
BstF5I BstNI BstXI BstYI BstZ17I
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PshBI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII
XmnI