##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII AclI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI AspEI AspI AviII
AvrII BalI BamHI BbrPI BclI
BfrI BglII BlnI BsaAI BsaBI
BsiWI BsmBI BsmI Bsp1407I BspLU11I
BsrGI BssHII Bst1107I BstBI BstZ17I
CpoI DraI DraIII DrdI Eam1105I
Eco47III EcoRI EcoT22I FbaI FseI
FspI HgaI HindIII HpaI MaeII
MamI MfeI MluI MluNI MscI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SapI ScaI SfuI SgrAI
SnaBI SpeI SphI SspBI SspI
SwaI Tth111I XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 1239 GGTACC G^GTACC
AccI 1398 GTCGAC GT^[AC][GT]AC
AccIII 1189 TCCGGA T^CCGGA
AcsI 656 AAATTC [GA]^AATT[CT]
AocI 1747 CCTCAGG CC^T[ACGT]AGG
ApaI 1585 GGGCCC GGGCC^C
ApoI 656 AAATTC [GA]^AATT[CT]
Asp700I 337 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1239 GGTACC G^GTACC
AvaI 387 CCCGGG C^[CT]CG[GA]G
BbsI 1496 GTCTTC GAAGAC(2/6)
BcgI 918 CGAAGGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 464 GTATCC GTATCC(6/5)
BpuAI 1496 GTCTTC GAAGAC(2/6)
BsaI 860 GAGACC GGTCTC(1/5)
BsaWI 1189 TCCGGA [AT]^CCGG[AT]
BseAI 1189 TCCGGA T^CCGGA
BsiEI 1424 CGGCCG CG[GA][CT]^CG
BsoBI 387 CCCGGG C^[CT]CG[GA]G
BspDI 1415 ATCGAT AT^CGAT
BspEI 1189 TCCGGA T^CCGGA
BspHI 945 TCATGA T^CATGA
BsrBI 235 CCGCTC CCGCTC(-3/-3)
BssSI 1580 CTCGTG CACGAG(-5/-1)
BstAPI 982 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 556 GGTGACC G^GT[ACGT]ACC
BstPI 556 GGTGACC G^GT[ACGT]ACC
Bsu36I 1747 CCTCAGG CC^T[ACGT]AGG
ClaI 1415 ATCGAT AT^CGAT
DsaI 29 CCGCGG C^C[GA][CT]GG
EaeI 1424 CGGCCG [CT]^GGCC[GA]
EagI 1424 CGGCCG C^GGCCG
EclXI 1424 CGGCCG C^GGCCG
Eco52I 1424 CGGCCG C^GGCCG
Eco81I 1747 CCTCAGG CC^T[ACGT]AGG
EcoO65I 556 GGTGACC G^GT[ACGT]ACC
EcoRV 1333 GATATC GAT^ATC
HincII 1398 GTCGAC GT[CT]^[GA]AC
HindII 1398 GTCGAC GT[CT]^[GA]AC
KpnI 1239 GGTACC GGTAC^C
KspI 29 CCGCGG CCGC^GG
MroI 1189 TCCGGA T^CCGGA
MseI 82 TTAA T^TAA
NspI 1059 ACATGC [GA]CATG^[CT]
PshAI 634 GACCTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 945 TCATGA T^CATGA
SacI 1183 GAGCTC GAGCT^C
SacII 29 CCGCGG CCGC^GG
SalI 1398 GTCGAC G^TCGAC
SbfI 925 CCTGCAGG CCTGCA^GG
SmaI 387 CCCGGG CCC^GGG
Sse8387I 925 CCTGCAGG CCTGCA^GG
StuI 285 AGGCCT AGG^CCT
Tru9I 82 TTAA T^TAA
XmaCI 387 CCCGGG C^CCGGG
XmaI 387 CCCGGG C^CCGGG
XmnI 337 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 1104 GGCGCC G[GA]^CG[CT]C
1449 GGCGCC
AspHI 1183 GAGCTC G[AT]GC[AT]^C
1430 GTGCTC
BbeI 1104 GGCGCC GGCGC^C
1449 GGCGCC
BglI 737 GCCTGTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
923 GCCCTGCAGGC
BlpI 823 GCTGAGC GC^T[ACGT]AGC
1670 GCTCAGC
Bpu1102I 823 GCTGAGC GC^T[ACGT]AGC
1670 GCTCAGC
BsaHI 1104 GGCGCC G[GA]^CG[CT]C
1449 GGCGCC
BsiHKAI 1183 GAGCTC G[AT]GC[AT]^C
1430 GTGCTC
BsrFI 1101 ACCGGC [GA]^CCGG[CT]
1426 GCCGGT
BstXI 402 CCAGTGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
561 CCAAGGATCTGG
CelII 823 GCTGAGC GC^T[ACGT]AGC
1670 GCTCAGC
Cfr10I 1101 ACCGGC [GA]^CCGG[CT]
1426 GCCGGT
EarI 1856 CTCTTC CTCTTC(1/4)
535 GAAGAG
EcoNI 676 CCTTCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
961 CCTGCTGGAGG
FauI 1222 GCGGG CCCGC(4/6)
1678 GCGGG
Hin1I 1104 GGCGCC G[GA]^CG[CT]C
1449 GGCGCC
KasI 1104 GGCGCC G^GCGCC
1449 GGCGCC
Ksp632I 1856 CTCTTC CTCTTC(1/4)
535 GAAGAG
MaeIII 253 GTCAC ^GT[ACGT]AC
557 GTGAC
NarI 1104 GGCGCC GG^CGCC
1449 GGCGCC
PstI 585 CTGCAG CTGCA^G
926 CTGCAG
SexAI 90 ACCTGGT A^CC[AT]GGT
1893 ACCAGGT
SfcI 585 CTGCAG C^T[GA][CT]AG
926 CTGCAG
SfiI 736 GGCCTGTCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
922 GGCCCTGCAGGCC
SfoI 1104 GGCGCC GGC^GCC
1449 GGCGCC
SmlI 1052 CTCAAG C^T[CT][GA]AG
1624 CTTGAG
Tsp45I 253 GTCAC ^GT[GC]AC
557 GTGAC
Tsp509I 38 AATT ^AATT
657 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AvaII BanI BanII BbvCI
BbvI BcnI BfaI BmyI BpmI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TfiI TseI TspRI
TthHB8I Van91I XhoII