##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
Acc65I AclI AflII AgeI Alw44I
ApaLI AscI AseI AsnI Asp718I
BcgI BciVI BclI BfrI BsiWI
BsmI Bsp1407I BsrGI Bst1107I BstAPI
BstBI BstZ17I CpoI DraI EcoRI
EcoRV EcoT22I FbaI HpaI KpnI
MfeI MluI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SapI SexAI SfiI SfuI
SgrAI SnaBI SpeI SspBI SwaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 2910 GACGTC GACGT^C
AflIII 3664 ACATGT A^C[GA][CT]GT
AhdI 4611 GACGGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1952 CCTTAGG CC^T[ACGT]AGG
Aor51HI 4175 AGCGCT AGC^GCT
AspEI 4611 GACGGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 6595 CCTAGG C^CTAGG
BbrPI 5547 CACGTG CAC^GTG
BlnI 6595 CCTAGG C^CTAGG
BsaAI 5547 CACGTG [CT]AC^GT[GA]
BsaI 1875 GAGACC GGTCTC(1/5)
BspHI 4664 TCATGA T^CATGA
BspLU11I 3664 ACATGT A^CATGT
BsrDI 5912 CATTGC GCAATG(2/0)
BssHII 109 GCGCGC G^CGCGC
Bsu36I 1952 CCTTAGG CC^T[ACGT]AGG
DraIII 4513 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4286 GACGGTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2956 CGGCCG C^GGCCG
Eam1105I 4611 GACGGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2956 CGGCCG C^GGCCG
Eco47III 4175 AGCGCT AGC^GCT
Eco52I 2956 CGGCCG C^GGCCG
Eco81I 1952 CCTTAGG CC^T[ACGT]AGG
FseI 679 GGCCGGCC GGCCGG^CC
HindIII 6297 AAGCTT A^AGCTT
KspI 3 CCGCGG CCGC^GG
NcoI 649 CCATGG C^CATGG
PaeR7I 3435 CTCGAG C^TCGAG
PmaCI 5547 CACGTG CAC^GTG
PmlI 5547 CACGTG CAC^GTG
RcaI 4664 TCATGA T^CATGA
SacII 3 CCGCGG CCGC^GG
SbfI 497 CCTGCAGG CCTGCA^GG
ScaI 1966 AGTACT AGT^ACT
SphI 6049 GCATGC GCATG^C
Sse8387I 497 CCTGCAGG CCTGCA^GG
SspI 5437 AATATT AAT^ATT
XbaI 861 TCTAGA T^CTAGA
XhoI 3435 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AviII 2422 TGCGCA TGC^GCA
3328 TGCGCA
BbvCI 2805 GCTGAGG CCTCAGC(-5/-2)
6496 GCTGAGG
BsaBI 1616 GATTCGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3815 GATCGAGATC
BsiEI 2956 CGGCCG CG[GA][CT]^CG
5242 CGGTCG
BspDI 4410 ATCGAT AT^CGAT
4680 ATCGAT
BstEII 962 GGTTACC G^GT[ACGT]ACC
3027 GGTCACC
BstPI 962 GGTTACC G^GT[ACGT]ACC
3027 GGTCACC
ClaI 4410 ATCGAT AT^CGAT
4680 ATCGAT
EcoO65I 962 GGTTACC G^GT[ACGT]ACC
3027 GGTCACC
FspI 2422 TGCGCA TGC^GCA
3328 TGCGCA
MamI 1616 GATTCGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3815 GATCGAGATC
MseI 3778 TTAA T^TAA
4891 TTAA
PshAI 3021 GACGCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5240 GACGGTCGTC
SalI 5247 GTCGAC G^TCGAC
5616 GTCGAC
StuI 4795 AGGCCT AGG^CCT
6598 AGGCCT
Tru9I 3778 TTAA T^TAA
4891 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 147 enzymes) #
#########################################
AccI AccII AccIII AciI AcsI
AcyI AfaI AluI AlwI AlwNI
ApaI ApoI Asp700I AspHI AspI
AvaI AvaII BalI BamHI BanI
BanII BbeI BbsI BbvI BcnI
BfaI BglI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaJI
BsaWI BseAI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspEI BspMI BsrBI
BsrFI BsrI BssKI BssSI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MroI MscI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PflFI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII XmaCI
XmaI XmnI