##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII Acc65I AflII AhdI AocI
Aor51HI ApaI AscI Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BbrPI BcgI BfrI BlnI BlpI
Bpu1102I BsiWI BsmI Bsp1407I BspDI
BsrGI BssHII Bst1107I BstZ17I Bsu36I
CelII ClaI CpoI DrdI Eam1105I
Eco47III Eco81I FseI FspI HincII
HindII HpaI KasI KpnI KspI
MluI NarI NdeI NheI NotI
PacI PaeR7I PflFI PmaCI PmeI
PmlI PvuI RsrII SacII SalI
SfoI SgrAI SspBI SspI StuI
SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccIII 648 TCCGGA T^CCGGA
AclI 1730 AACGTT AA^CGTT
AgeI 1923 ACCGGT A^CCGGT
Alw44I 3257 GTGCAC G^TGCAC
ApaLI 3257 GTGCAC G^TGCAC
AvaI 61 CCCGGG C^[CT]CG[GA]G
BalI 455 TGGCCA TGG^CCA
BciVI 4858 GTATCC GTATCC(6/5)
BsaAI 3544 TACGTA [CT]AC^GT[GA]
BseAI 648 TCCGGA T^CCGGA
BsmBI 99 GAGACG CGTCTC(1/5)
BsoBI 61 CCCGGG C^[CT]CG[GA]G
BspEI 648 TCCGGA T^CCGGA
BspHI 4623 TCATGA T^CATGA
BspLU11I 4540 ACATGT A^CATGT
BsrBI 383 GAGCGG CCGCTC(-3/-3)
BssSI 2971 CACGAG CACGAG(-5/-1)
BstEII 3286 GGTTACC G^GT[ACGT]ACC
BstPI 3286 GGTTACC G^GT[ACGT]ACC
BstXI 6546 CCATTTATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI 85 CGGCCG C^GGCCG
EclXI 85 CGGCCG C^GGCCG
Eco52I 85 CGGCCG C^GGCCG
EcoNI 333 CCTTTGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3286 GGTTACC G^GT[ACGT]ACC
EcoRV 5595 GATATC GAT^ATC
MluNI 455 TGGCCA TGG^CCA
MroI 648 TCCGGA T^CCGGA
MscI 455 TGGCCA TGG^CCA
NaeI 297 GCCGGC GCC^GGC
NcoI 4198 CCATGG C^CATGG
NgoMIV 297 GCCGGC G^CCGGC
NruI 1089 TCGCGA TCG^CGA
PflMI 661 CCAGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1923 ACCGGT A^CCGGT
PshAI 30 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1730 AACGTT AA^CGTT
RcaI 4623 TCATGA T^CATGA
SbfI 5198 CCTGCAGG CCTGCA^GG
ScaI 1888 AGTACT AGT^ACT
SexAI 3978 ACCAGGT A^CC[AT]GGT
SfiI 228 GGCCACTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 61 CCCGGG CCC^GGG
SnaBI 3544 TACGTA TAC^GTA
Sse8387I 5198 CCTGCAGG CCTGCA^GG
Van91I 661 CCAGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 248 CCAGGAAACGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 61 CCCGGG C^CCGGG
XmaI 61 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 3246 GTCTAC GT^[AC][GT]AC
4659 GTCTAC
AcyI 1561 GGCGTC G[GA]^CG[CT]C
4319 GACGCC
AflIII 255 ACGTGT A^C[GA][CT]GT
4540 ACATGT
BclI 492 TGATCA T^GATCA
5874 TGATCA
BcnI 61 CCCGG CC^[GC]GG
62 CCGGG
BsaHI 1561 GGCGTC G[GA]^CG[CT]C
4319 GACGCC
BsaI 2974 GAGACC GGTCTC(1/5)
276 GGTCTC
BsaWI 648 TCCGGA [AT]^CCGG[AT]
1923 ACCGGT
BsiEI 85 CGGCCG CG[GA][CT]^CG
289 CGACCG
BstBI 2057 TTCGAA TT^CGAA
2213 TTCGAA
DraI 6097 TTTAAA TTT^AAA
6216 TTTAAA
DsaI 1349 CCACGG C^C[GA][CT]GG
4198 CCATGG
EcoRI 4074 GAATTC G^AATTC
4476 GAATTC
FbaI 492 TGATCA T^GATCA
5874 TGATCA
HaeII 200 GGCGCT [GA]GCGC^[CT]
1163 AGCGCC
Hin1I 1561 GGCGTC G[GA]^CG[CT]C
4319 GACGCC
MfeI 2812 CAATTG C^AATTG
3435 CAATTG
MunI 2812 CAATTG C^AATTG
3435 CAATTG
NciI 61 CCCGG CC^[GC]GG
62 CCGGG
NspV 2057 TTCGAA TT^CGAA
2213 TTCGAA
SacI 1451 GAGCTC GAGCT^C
3535 GAGCTC
SapI 3331 GAAGAGC GCTCTTC(1/4)
4585 GAAGAGC
SfuI 2057 TTCGAA TT^CGAA
2213 TTCGAA
SpeI 416 ACTAGT A^CTAGT
6145 ACTAGT
SphI 2875 GCATGC GCATG^C
3500 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AseI AsnI
Asp700I AspHI AvaII BanI BanII
BbsI BbvCI BbvI BfaI BglI
BglII BmyI BpmI BpuAI BsaBI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII EaeI EarI EcoO109I
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
NsiI NspI PleI PpuMI PshBI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI