##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
Acc65I AclI AgeI Aor51HI AscI
AseI AsnI Asp718I AviII BbeI
BsaBI BsiWI BspDI BssHII BssSI
BstBI ClaI DraI DrdI Eco47III
FseI FspI HaeII HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MunI NarI
NheI NotI NruI NspV PacI
PinAI PmeI PshBI Psp1406I SacII
SalI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SwaI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AflII 1626 CTTAAG C^TTAAG
AspI 6225 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1334 CCTAGG C^CTAGG
BcgI 5059 CGACTCCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3510 GTATCC GTATCC(6/5)
BclI 395 TGATCA T^GATCA
BfrI 1626 CTTAAG C^TTAAG
BlnI 1334 CCTAGG C^CTAGG
Bst1107I 2501 GTATAC GTA^TAC
BstZ17I 2501 GTATAC GTA^TAC
CpoI 228 CGGTCCG CG^G[AT]CCG
EagI 64 CGGCCG C^GGCCG
EclXI 64 CGGCCG C^GGCCG
Eco52I 64 CGGCCG C^GGCCG
EcoRV 669 GATATC GAT^ATC
FbaI 395 TGATCA T^GATCA
NaeI 62 GCCGGC GCC^GGC
NgoMIV 62 GCCGGC G^CCGGC
PaeR7I 2578 CTCGAG C^TCGAG
PflFI 6225 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1337 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 444 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 936 CGATCG CGAT^CG
RsrII 228 CGGTCCG CG^G[AT]CCG
SbfI 1667 CCTGCAGG CCTGCA^GG
Sse8387I 1667 CCTGCAGG CCTGCA^GG
SspI 5717 AATATT AAT^ATT
Tth111I 6225 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2578 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AatII 1986 GACGTC GACGT^C
2385 GACGTC
AccIII 3643 TCCGGA T^CCGGA
4960 TCCGGA
Alw44I 912 GTGCAC G^TGCAC
1694 GTGCAC
ApaLI 912 GTGCAC G^TGCAC
1694 GTGCAC
BbvCI 4042 CCTCAGC CCTCAGC(-5/-2)
1893 GCTGAGG
BsaI 855 GAGACC GGTCTC(1/5)
5988 GGTCTC
BseAI 3643 TCCGGA T^CCGGA
4960 TCCGGA
Bsp1407I 4453 TGTACA T^GTACA
6071 TGTACA
BspEI 3643 TCCGGA T^CCGGA
4960 TCCGGA
BspHI 5224 TCATGA T^CATGA
5671 TCATGA
BsrBI 225 GAGCGG CCGCTC(-3/-3)
1020 GAGCGG
BsrGI 4453 TGTACA T^GTACA
6071 TGTACA
BstEII 1455 GGTGACC G^GT[ACGT]ACC
5274 GGTTACC
BstPI 1455 GGTGACC G^GT[ACGT]ACC
5274 GGTTACC
DraIII 4118 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
5690 CACGTGGTG
EcoNI 3995 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
6040 CCTATAGAAGG
EcoO65I 1455 GGTGACC G^GT[ACGT]ACC
5274 GGTTACC
EcoRI 3224 GAATTC G^AATTC
3519 GAATTC
MroI 3643 TCCGGA T^CCGGA
4960 TCCGGA
NcoI 493 CCATGG C^CATGG
3844 CCATGG
PflMI 688 CCAGATATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1446 CCAGATGATGG
RcaI 5224 TCATGA T^CATGA
5671 TCATGA
SacI 3924 GAGCTC GAGCT^C
4158 GAGCTC
SmaI 970 CCCGGG CCC^GGG
4834 CCCGGG
SspBI 4453 TGTACA T^GTACA
6071 TGTACA
Van91I 688 CCAGATATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1446 CCAGATGATGG
XbaI 5631 TCTAGA T^CTAGA
5913 TCTAGA
XmaCI 970 CCCGGG C^CCGGG
4834 CCCGGG
XmaI 970 CCCGGG C^CCGGG
4834 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 145 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AhdI AluI AlwI
AlwNI AocI ApaI ApoI Asp700I
AspEI AspHI AvaI AvaII BalI
BamHI BanI BanII BbrPI BbsI
BbvI BcnI BfaI BglI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I BspLU11I BspMI BsrDI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI Eam1105I
EarI Eco81I EcoO109I EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeI NdeII NlaIII
NlaIV NsiI NspI PleI PmaCI
PmlI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmnI