##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccII AcyI AflII
AgeI Alw44I AlwNI Aor51HI ApaI
ApaLI AscI Asp718I AspI AvaI
AviII AvrII BbeI BbrPI BciVI
BfrI BglI BlnI BsaHI BsiWI
BsoBI BsrBI BsrFI BssHII BssSI
BstAPI BstUI Cfr10I CpoI DraII
DraIII Eco47III EcoO109I FseI FspI
Hin1I KasI KpnI KspI MluI
MvnI NaeI NarI NcoI NgoMIV
NotI NruI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PvuI RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SgrAI SmaI SnaBI Sse8387I SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccIII 211 TCCGGA T^CCGGA
AclI 2853 AACGTT AA^CGTT
AhdI 5391 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 5391 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 3255 GTGCTC G[AT]GC[AT]^C
BanI 5835 GGTGCC G^G[CT][GA]CC
BclI 3013 TGATCA T^GATCA
BsaAI 1477 TACGTG [CT]AC^GT[GA]
BsaWI 211 TCCGGA [AT]^CCGG[AT]
BseAI 211 TCCGGA T^CCGGA
BsiEI 6417 CGGCCG CG[GA][CT]^CG
BsiHKAI 3255 GTGCTC G[AT]GC[AT]^C
BspDI 428 ATCGAT AT^CGAT
BspEI 211 TCCGGA T^CCGGA
BspHI 1627 TCATGA T^CATGA
Bst1107I 5042 GTATAC GTA^TAC
BstEII 4182 GGTTACC G^GT[ACGT]ACC
BstPI 4182 GGTTACC G^GT[ACGT]ACC
BstZ17I 5042 GTATAC GTA^TAC
ClaI 428 ATCGAT AT^CGAT
DsaI 1740 CCGTGG C^C[GA][CT]GG
EagI 6417 CGGCCG C^GGCCG
Eam1105I 5391 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 6417 CGGCCG C^GGCCG
Eco52I 6417 CGGCCG C^GGCCG
EcoNI 4820 CCTACTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 4182 GGTTACC G^GT[ACGT]ACC
EcoRI 6224 GAATTC G^AATTC
FbaI 3013 TGATCA T^GATCA
HaeII 2903 AGCGCC [GA]GCGC^[CT]
HgaI 4250 GACGC GACGC(5/10)
HpaI 6166 GTTAAC GTT^AAC
MfeI 2417 CAATTG C^AATTG
MroI 211 TCCGGA T^CCGGA
MunI 2417 CAATTG C^AATTG
NheI 1054 GCTAGC G^CTAGC
PshAI 6256 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2853 AACGTT AA^CGTT
RcaI 1627 TCATGA T^CATGA
SapI 1850 GCTCTTC GCTCTTC(1/4)
SpeI 5222 ACTAGT A^CTAGT
SphI 5267 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 5042 GTATAC GT^[AC][GT]AC
6269 GTAGAC
AseI 3557 ATTAAT AT^TAAT
4055 ATTAAT
AsnI 3557 ATTAAT AT^TAAT
4055 ATTAAT
BalI 584 TGGCCA TGG^CCA
6508 TGGCCA
BbvCI 16 GCTGAGG CCTCAGC(-5/-2)
6591 GCTGAGG
BcgI 1933 CGAGACTCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
6597 GCAGGAGAATCG
BcnI 728 CCGGG CC^[GC]GG
6420 CCGGG
BlpI 7 GCTGAGC GC^T[ACGT]AGC
4340 GCTCAGC
BpmI 2216 CTCCAG CTGGAG(16/14)
4824 CTCCAG
Bpu1102I 7 GCTGAGC GC^T[ACGT]AGC
4340 GCTCAGC
BsaI 6498 GAGACC GGTCTC(1/5)
1435 GGTCTC
BsgI 6654 CTGCAC GTGCAG(16/14)
5103 GTGCAG
BspMI 763 ACCTGC ACCTGC(4/8)
4805 GCAGGT
BstBI 1182 TTCGAA TT^CGAA
3933 TTCGAA
CelII 7 GCTGAGC GC^T[ACGT]AGC
4340 GCTCAGC
CfoI 1397 GCGC GCG^C
2904 GCGC
DrdI 4416 GACAAGATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6272 GACCAGTTTGTC
EcoT22I 4895 ATGCAT ATGCA^T
5269 ATGCAT
HhaI 1397 GCGC GCG^C
2904 GCGC
HinP1I 1397 GCGC G^CGC
2904 GCGC
HincII 2720 GTTGAC GT[CT]^[GA]AC
6166 GTTAAC
HindII 2720 GTTGAC GT[CT]^[GA]AC
6166 GTTAAC
MluNI 584 TGGCCA TGG^CCA
6508 TGGCCA
MscI 584 TGGCCA TGG^CCA
6508 TGGCCA
NciI 728 CCGGG CC^[GC]GG
6420 CCGGG
NsiI 4895 ATGCAT ATGCA^T
5269 ATGCAT
NspV 1182 TTCGAA TT^CGAA
3933 TTCGAA
PshBI 3557 ATTAAT AT^TAAT
4055 ATTAAT
ScaI 818 AGTACT AGT^ACT
5515 AGTACT
SfuI 1182 TTCGAA TT^CGAA
3933 TTCGAA
StuI 94 AGGCCT AGG^CCT
4113 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AocI ApoI Asp700I AvaII
BamHI BanII BbsI BbvI BfaI
BglII BmyI BpuAI BsaBI BsaJI
BseRI BsiYI BslI BsmAI BsmBI
BsmFI BsmI Bsp1286I Bsp1407I BspLU11I
BsrDI BsrGI BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
Cfr13I DdeI DpnII DraI EaeI
EarI Eco81I EcoRII EcoRV EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NdeI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmnI