##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
Acc65I AccIII AclI AhdI Alw44I
Aor51HI ApaLI AscI Asp718I AspEI
AvrII BbrPI BclI BglI BlnI
BsaAI BseAI BsmBI BspDI BspEI
BssHII ClaI Eam1105I Eco47III FbaI
FseI KpnI KspI MfeI MluI
MroI MunI NaeI NgoMIV NheI
NotI NruI PacI PmaCI PmeI
PmlI Psp1406I PvuI SacII SalI
SbfI ScaI SfiI SgrAI SmaI
SnaBI Sse8387I SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AgeI 170 ACCGGT A^CCGGT
ApaI 943 GGGCCC GGGCC^C
AspI 876 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 5038 TGCGCA TGC^GCA
BbeI 2027 GGCGCC GGCGC^C
BcgI 6155 GCATGCTTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3901 GTATCC GTATCC(6/5)
BsaBI 4344 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 3921 CGTACG C^GTACG
Bst1107I 1290 GTATAC GTA^TAC
BstBI 2552 TTCGAA TT^CGAA
BstZ17I 1290 GTATAC GTA^TAC
CpoI 3439 CGGACCG CG^G[AT]CCG
DraIII 3115 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 118 CCTCTGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 214 GCGGG CCCGC(4/6)
FspI 5038 TGCGCA TGC^GCA
HincII 1072 GTTAAC GT[CT]^[GA]AC
HindII 1072 GTTAAC GT[CT]^[GA]AC
HindIII 408 AAGCTT A^AGCTT
HpaI 1072 GTTAAC GTT^AAC
KasI 2027 GGCGCC G^GCGCC
MamI 4344 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 2027 GGCGCC GG^CGCC
NspV 2552 TTCGAA TT^CGAA
PflFI 876 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 170 ACCGGT A^CCGGT
PpuMI 184 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 5331 GACCTTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 3439 CGGACCG CG^G[AT]CCG
SexAI 5752 ACCTGGT A^CC[AT]GGT
SfoI 2027 GGCGCC GGC^GCC
SfuI 2552 TTCGAA TT^CGAA
SpeI 1374 ACTAGT A^CTAGT
SspI 5503 AATATT AAT^ATT
StuI 2690 AGGCCT AGG^CCT
Tth111I 876 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 5947 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AatII 1994 GACGTC GACGT^C
5814 GACGTC
AflII 3394 CTTAAG C^TTAAG
6853 CTTAAG
AocI 790 CCTGAGG CC^T[ACGT]AGG
2022 CCTAAGG
AseI 1585 ATTAAT AT^TAAT
5582 ATTAAT
AsnI 1585 ATTAAT AT^TAAT
5582 ATTAAT
BamHI 3808 GGATCC G^GATCC
6073 GGATCC
BbvCI 5845 CCTCAGC CCTCAGC(-5/-2)
2518 GCTGAGG
BfrI 3394 CTTAAG C^TTAAG
6853 CTTAAG
BsaWI 170 ACCGGT [AT]^CCGG[AT]
1476 TCCGGT
Bsp1407I 1865 TGTACA T^GTACA
2169 TGTACA
BspMI 5912 GCAGGT ACCTGC(4/8)
6063 GCAGGT
BsrFI 170 ACCGGT [GA]^CCGG[CT]
346 ACCGGC
BsrGI 1865 TGTACA T^GTACA
2169 TGTACA
BssSI 2047 CACGAG CACGAG(-5/-1)
1910 CTCGTG
Bsu36I 790 CCTGAGG CC^T[ACGT]AGG
2022 CCTAAGG
Cfr10I 170 ACCGGT [GA]^CCGG[CT]
346 ACCGGC
Eco81I 790 CCTGAGG CC^T[ACGT]AGG
2022 CCTAAGG
EcoRI 701 GAATTC G^AATTC
3402 GAATTC
EcoT22I 3631 ATGCAT ATGCA^T
6153 ATGCAT
HaeII 434 AGCGCC [GA]GCGC^[CT]
2027 GGCGCC
NsiI 3631 ATGCAT ATGCA^T
6153 ATGCAT
PshBI 1585 ATTAAT AT^TAAT
5582 ATTAAT
SapI 4473 GAAGAGC GCTCTTC(1/4)
6058 GAAGAGC
SspBI 1865 TGTACA T^GTACA
2169 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApoI Asp700I AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspHI BspLU11I BsrBI
BsrDI BsrI BssKI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII DrdI
DsaI EaeI EagI EarI EclXI
Eco52I EcoO109I EcoO65I EcoRII EcoRV
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeI NdeII NlaIII
NlaIV NspI PaeR7I PflMI PleI
PstI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoI XhoII
XmnI