################################## # NO CUT ENZYMES ( 54 enzymes) # ################################## AatII Acc65I AccIII AclI AhdI Aor51HI AscI AseI AsnI Asp718I AspEI AviII AvrII BamHI BglII BlnI BseAI BsiWI BspEI BssHII Bst1107I BstBI BstZ17I CpoI Eam1105I Eco47III EcoRV FseI FspI KpnI MluI MroI NotI NruI NspV PacI PmeI PshAI PshBI Psp1406I PvuI RsrII SacI SapI SbfI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SwaI XbaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AflII 5028 CTTAAG C^TTAAG AgeI 2179 ACCGGT A^CCGGT BbeI 3608 GGCGCC GGCGC^C BbvCI 2836 CCTCAGC CCTCAGC(-5/-2) BcgI 4206 GCACGGGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 4233 GTATCC GTATCC(6/5) BclI 4386 TGATCA T^GATCA BfrI 5028 CTTAAG C^TTAAG BsaBI 2425 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmI 2700 GCATTC GAATGC(1/-1) BspHI 4081 TCATGA T^CATGA BspLU11I 5470 ACATGT A^CATGT BsrBI 5356 CCGCTC CCGCTC(-3/-3) BsrDI 841 CATTGC GCAATG(2/0) DraI 5719 TTTAAA TTT^AAA EcoNI 17 CCTGTGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 4244 GAATTC G^AATTC EcoT22I 2187 ATGCAT ATGCA^T FbaI 4386 TGATCA T^GATCA HindIII 5040 AAGCTT A^AGCTT HpaI 3938 GTTAAC GTT^AAC KasI 3608 GGCGCC G^GCGCC KspI 3061 CCGCGG CCGC^GG MamI 2425 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1656 CAATTG C^AATTG MunI 1656 CAATTG C^AATTG NarI 3608 GGCGCC GG^CGCC NheI 5173 GCTAGC G^CTAGC NsiI 2187 ATGCAT ATGCA^T PaeR7I 461 CTCGAG C^TCGAG PinAI 2179 ACCGGT A^CCGGT RcaI 4081 TCATGA T^CATGA SacII 3061 CCGCGG CCGC^GG SalI 500 GTCGAC G^TCGAC ScaI 351 AGTACT AGT^ACT SfoI 3608 GGCGCC GGC^GCC SspI 4958 AATATT AAT^ATT XhoI 461 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### Alw44I 3524 GTGCAC G^TGCAC 4001 GTGCAC AocI 852 CCTCAGG CC^T[ACGT]AGG 6125 CCTGAGG ApaLI 3524 GTGCAC G^TGCAC 4001 GTGCAC AspI 2276 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4513 GACCAGGTC BbsI 2341 GAAGAC GAAGAC(2/6) 1076 GTCTTC BglI 1416 GCCCACGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3438 GCCCCTCTGGC BlpI 2796 GCTCAGC GC^T[ACGT]AGC 4473 GCTCAGC Bpu1102I 2796 GCTCAGC GC^T[ACGT]AGC 4473 GCTCAGC BpuAI 2341 GAAGAC GAAGAC(2/6) 1076 GTCTTC BsaWI 2179 ACCGGT [AT]^CCGG[AT] 3498 TCCGGT BsgI 1981 CTGCAC GTGCAG(16/14) 5970 GTGCAG BsiEI 627 CGGCCG CG[GA][CT]^CG 800 CGGCCG BspDI 221 ATCGAT AT^CGAT 4103 ATCGAT Bsu36I 852 CCTCAGG CC^T[ACGT]AGG 6125 CCTGAGG CelII 2796 GCTCAGC GC^T[ACGT]AGC 4473 GCTCAGC ClaI 221 ATCGAT AT^CGAT 4103 ATCGAT DrdI 2375 GACGGCACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4513 GACCAGGTCGTC EagI 627 CGGCCG C^GGCCG 800 CGGCCG EclXI 627 CGGCCG C^GGCCG 800 CGGCCG Eco52I 627 CGGCCG C^GGCCG 800 CGGCCG Eco81I 852 CCTCAGG CC^T[ACGT]AGG 6125 CCTGAGG NaeI 629 GCCGGC GCC^GGC 1313 GCCGGC NgoMIV 629 GCCGGC G^CCGGC 1313 GCCGGC PflFI 2276 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4513 GACCAGGTC Tth111I 2276 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4513 GACCAGGTC ######################################### # 3 OR MORE CUT ENZYMES ( 141 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApaI ApoI Asp700I AspHI AvaI AvaII BalI BanI BanII BbrPI BbvI BcnI BfaI BmyI BpmI BsaAI BsaHI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrFI BsrGI BsrI BssKI BssSI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeI NdeII NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmaI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI XmnI