##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AatII Acc65I AccIII AclI AhdI
Aor51HI AscI AseI AsnI Asp718I
AspEI AviII AvrII BamHI BglII
BlnI BseAI BsiWI BspEI BssHII
Bst1107I BstBI BstZ17I CpoI Eam1105I
Eco47III EcoRV FseI FspI KpnI
MluI MroI NotI NruI NspV
PacI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacI SapI SbfI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SwaI XbaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AflII 5028 CTTAAG C^TTAAG
AgeI 2179 ACCGGT A^CCGGT
BbeI 3608 GGCGCC GGCGC^C
BbvCI 2836 CCTCAGC CCTCAGC(-5/-2)
BcgI 4206 GCACGGGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4233 GTATCC GTATCC(6/5)
BclI 4386 TGATCA T^GATCA
BfrI 5028 CTTAAG C^TTAAG
BsaBI 2425 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 2700 GCATTC GAATGC(1/-1)
BspHI 4081 TCATGA T^CATGA
BspLU11I 5470 ACATGT A^CATGT
BsrBI 5356 CCGCTC CCGCTC(-3/-3)
BsrDI 841 CATTGC GCAATG(2/0)
DraI 5719 TTTAAA TTT^AAA
EcoNI 17 CCTGTGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 4244 GAATTC G^AATTC
EcoT22I 2187 ATGCAT ATGCA^T
FbaI 4386 TGATCA T^GATCA
HindIII 5040 AAGCTT A^AGCTT
HpaI 3938 GTTAAC GTT^AAC
KasI 3608 GGCGCC G^GCGCC
KspI 3061 CCGCGG CCGC^GG
MamI 2425 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1656 CAATTG C^AATTG
MunI 1656 CAATTG C^AATTG
NarI 3608 GGCGCC GG^CGCC
NheI 5173 GCTAGC G^CTAGC
NsiI 2187 ATGCAT ATGCA^T
PaeR7I 461 CTCGAG C^TCGAG
PinAI 2179 ACCGGT A^CCGGT
RcaI 4081 TCATGA T^CATGA
SacII 3061 CCGCGG CCGC^GG
SalI 500 GTCGAC G^TCGAC
ScaI 351 AGTACT AGT^ACT
SfoI 3608 GGCGCC GGC^GCC
SspI 4958 AATATT AAT^ATT
XhoI 461 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Alw44I 3524 GTGCAC G^TGCAC
4001 GTGCAC
AocI 852 CCTCAGG CC^T[ACGT]AGG
6125 CCTGAGG
ApaLI 3524 GTGCAC G^TGCAC
4001 GTGCAC
AspI 2276 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4513 GACCAGGTC
BbsI 2341 GAAGAC GAAGAC(2/6)
1076 GTCTTC
BglI 1416 GCCCACGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3438 GCCCCTCTGGC
BlpI 2796 GCTCAGC GC^T[ACGT]AGC
4473 GCTCAGC
Bpu1102I 2796 GCTCAGC GC^T[ACGT]AGC
4473 GCTCAGC
BpuAI 2341 GAAGAC GAAGAC(2/6)
1076 GTCTTC
BsaWI 2179 ACCGGT [AT]^CCGG[AT]
3498 TCCGGT
BsgI 1981 CTGCAC GTGCAG(16/14)
5970 GTGCAG
BsiEI 627 CGGCCG CG[GA][CT]^CG
800 CGGCCG
BspDI 221 ATCGAT AT^CGAT
4103 ATCGAT
Bsu36I 852 CCTCAGG CC^T[ACGT]AGG
6125 CCTGAGG
CelII 2796 GCTCAGC GC^T[ACGT]AGC
4473 GCTCAGC
ClaI 221 ATCGAT AT^CGAT
4103 ATCGAT
DrdI 2375 GACGGCACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4513 GACCAGGTCGTC
EagI 627 CGGCCG C^GGCCG
800 CGGCCG
EclXI 627 CGGCCG C^GGCCG
800 CGGCCG
Eco52I 627 CGGCCG C^GGCCG
800 CGGCCG
Eco81I 852 CCTCAGG CC^T[ACGT]AGG
6125 CCTGAGG
NaeI 629 GCCGGC GCC^GGC
1313 GCCGGC
NgoMIV 629 GCCGGC G^CCGGC
1313 GCCGGC
PflFI 2276 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4513 GACCAGGTC
Tth111I 2276 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4513 GACCAGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApaI ApoI Asp700I AspHI AvaI
AvaII BalI BanI BanII BbrPI
BbvI BcnI BfaI BmyI BpmI
BsaAI BsaHI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I Bsp1407I BspMI
BsrFI BsrGI BsrI BssKI BssSI
BstAPI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeI NdeII
NlaIII NlaIV NspI PflMI PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SmaI SmlI SspBI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI
XmnI