##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII AclI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
AspEI BclI BsiWI Bsp1407I BspDI
BsrDI BsrGI BssHII Bst1107I BstBI
BstZ17I ClaI CpoI Eam1105I Eco47III
EcoRV EcoT22I FbaI FseI HpaI
KspI MfeI MluI MunI NaeI
NgoMIV NheI NruI NsiI NspV
PacI PaeR7I PflMI PmeI PshBI
Psp1406I PvuI RsrII SacII SbfI
SfiI SfuI SnaBI SpeI Sse8387I
SspBI Van91I XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 3476 GGTACC G^GTACC
AccI 2294 GTCGAC GT^[AC][GT]AC
AccIII 2303 TCCGGA T^CCGGA
AflII 176 CTTAAG C^TTAAG
AocI 5815 CCTCAGG CC^T[ACGT]AGG
Asp718I 3476 GGTACC G^GTACC
AviII 2372 TGCGCA TGC^GCA
AvrII 4604 CCTAGG C^CTAGG
BamHI 857 GGATCC G^GATCC
BbrPI 393 CACGTG CAC^GTG
BbvCI 79 CCTCAGC CCTCAGC(-5/-2)
BcgI 3126 GCATTCGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 176 CTTAAG C^TTAAG
BlnI 4604 CCTAGG C^CTAGG
BsaBI 1141 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2303 TCCGGA T^CCGGA
BsgI 4373 CTGCAC GTGCAG(16/14)
BspEI 2303 TCCGGA T^CCGGA
BspLU11I 4802 ACATGT A^CATGT
Bsu36I 5815 CCTCAGG CC^T[ACGT]AGG
Eco81I 5815 CCTCAGG CC^T[ACGT]AGG
FspI 2372 TGCGCA TGC^GCA
HgaI 3429 GCGTC GACGC(5/10)
HindIII 4572 AAGCTT A^AGCTT
KpnI 3476 GGTACC GGTAC^C
MamI 1141 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2303 TCCGGA T^CCGGA
NdeI 1108 CATATG CA^TATG
NotI 13 GCGGCCGC GC^GGCCGC
PmaCI 393 CACGTG CAC^GTG
PmlI 393 CACGTG CAC^GTG
PpuMI 2525 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 3424 GACAAGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 2294 GTCGAC G^TCGAC
ScaI 283 AGTACT AGT^ACT
SphI 4552 GCATGC GCATG^C
SwaI 1657 ATTTAAAT ATTT^AAAT
XbaI 4457 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 2960 GGCGCC G[GA]^CG[CT]C
5643 GGCGCC
AflIII 4802 ACATGT A^C[GA][CT]GT
5318 ACGTGT
AgeI 217 ACCGGT A^CCGGT
2601 ACCGGT
BbeI 2960 GGCGCC GGCGC^C
5643 GGCGCC
BciVI 86 GGATAC GTATCC(6/5)
1449 GTATCC
BglI 16 GCCGCACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2081 GCCATGAAGGC
BglII 772 AGATCT A^GATCT
5941 AGATCT
BsaHI 2960 GGCGCC G[GA]^CG[CT]C
5643 GGCGCC
BsmBI 1952 CGTCTC CGTCTC(1/5)
3632 GAGACG
BsrBI 1179 CCGCTC CCGCTC(-3/-3)
2260 CCGCTC
BsrFI 217 ACCGGT [GA]^CCGG[CT]
2601 ACCGGT
BstAPI 2405 GCAGCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4345 GCAGATAATGC
Cfr10I 217 ACCGGT [GA]^CCGG[CT]
2601 ACCGGT
EcoNI 143 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1024 CCTTACCAAGG
HaeII 2960 GGCGCC [GA]GCGC^[CT]
5643 GGCGCC
Hin1I 2960 GGCGCC G[GA]^CG[CT]C
5643 GGCGCC
KasI 2960 GGCGCC G^GCGCC
5643 GGCGCC
NarI 2960 GGCGCC GG^CGCC
5643 GGCGCC
NcoI 2115 CCATGG C^CATGG
2266 CCATGG
NspI 4552 GCATGC [GA]CATG^[CT]
4802 ACATGT
PinAI 217 ACCGGT A^CCGGT
2601 ACCGGT
SacI 2207 GAGCTC GAGCT^C
5867 GAGCTC
SexAI 1462 ACCAGGT A^CC[AT]GGT
4750 ACCTGGT
SfoI 2960 GGCGCC GGC^GCC
5643 GGCGCC
SgrAI 216 CACCGGTG C[GA]^CCGG[CT]G
2600 CACCGGTG
SmaI 67 CCCGGG CCC^GGG
3516 CCCGGG
StuI 3658 AGGCCT AGG^CCT
5667 AGGCCT
XmaCI 67 CCCGGG C^CCGGG
3516 CCCGGG
XmaI 67 CCCGGG C^CCGGG
3516 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AspI AvaI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaI BsaJI BsaWI BseRI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspHI
BspMI BsrI BssKI BssSI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII DraIII
DrdI DsaI EaeI EagI EarI
EclXI Eco52I EcoO109I EcoO65I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflFI PleI PstI PvuII
RcaI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
XcmI XhoII XmnI