################################## # NO CUT ENZYMES ( 58 enzymes) # ################################## AatII AclI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI AspEI BclI BsiWI Bsp1407I BspDI BsrDI BsrGI BssHII Bst1107I BstBI BstZ17I ClaI CpoI Eam1105I Eco47III EcoRV EcoT22I FbaI FseI HpaI KspI MfeI MluI MunI NaeI NgoMIV NheI NruI NsiI NspV PacI PaeR7I PflMI PmeI PshBI Psp1406I PvuI RsrII SacII SbfI SfiI SfuI SnaBI SpeI Sse8387I SspBI Van91I XhoI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### Acc65I 3476 GGTACC G^GTACC AccI 2294 GTCGAC GT^[AC][GT]AC AccIII 2303 TCCGGA T^CCGGA AflII 176 CTTAAG C^TTAAG AocI 5815 CCTCAGG CC^T[ACGT]AGG Asp718I 3476 GGTACC G^GTACC AviII 2372 TGCGCA TGC^GCA AvrII 4604 CCTAGG C^CTAGG BamHI 857 GGATCC G^GATCC BbrPI 393 CACGTG CAC^GTG BbvCI 79 CCTCAGC CCTCAGC(-5/-2) BcgI 3126 GCATTCGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 176 CTTAAG C^TTAAG BlnI 4604 CCTAGG C^CTAGG BsaBI 1141 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 2303 TCCGGA T^CCGGA BsgI 4373 CTGCAC GTGCAG(16/14) BspEI 2303 TCCGGA T^CCGGA BspLU11I 4802 ACATGT A^CATGT Bsu36I 5815 CCTCAGG CC^T[ACGT]AGG Eco81I 5815 CCTCAGG CC^T[ACGT]AGG FspI 2372 TGCGCA TGC^GCA HgaI 3429 GCGTC GACGC(5/10) HindIII 4572 AAGCTT A^AGCTT KpnI 3476 GGTACC GGTAC^C MamI 1141 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 2303 TCCGGA T^CCGGA NdeI 1108 CATATG CA^TATG NotI 13 GCGGCCGC GC^GGCCGC PmaCI 393 CACGTG CAC^GTG PmlI 393 CACGTG CAC^GTG PpuMI 2525 AGGTCCC [GA]G^G[AT]CC[CT] PshAI 3424 GACAAGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SalI 2294 GTCGAC G^TCGAC ScaI 283 AGTACT AGT^ACT SphI 4552 GCATGC GCATG^C SwaI 1657 ATTTAAAT ATTT^AAAT XbaI 4457 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcyI 2960 GGCGCC G[GA]^CG[CT]C 5643 GGCGCC AflIII 4802 ACATGT A^C[GA][CT]GT 5318 ACGTGT AgeI 217 ACCGGT A^CCGGT 2601 ACCGGT BbeI 2960 GGCGCC GGCGC^C 5643 GGCGCC BciVI 86 GGATAC GTATCC(6/5) 1449 GTATCC BglI 16 GCCGCACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2081 GCCATGAAGGC BglII 772 AGATCT A^GATCT 5941 AGATCT BsaHI 2960 GGCGCC G[GA]^CG[CT]C 5643 GGCGCC BsmBI 1952 CGTCTC CGTCTC(1/5) 3632 GAGACG BsrBI 1179 CCGCTC CCGCTC(-3/-3) 2260 CCGCTC BsrFI 217 ACCGGT [GA]^CCGG[CT] 2601 ACCGGT BstAPI 2405 GCAGCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4345 GCAGATAATGC Cfr10I 217 ACCGGT [GA]^CCGG[CT] 2601 ACCGGT EcoNI 143 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1024 CCTTACCAAGG HaeII 2960 GGCGCC [GA]GCGC^[CT] 5643 GGCGCC Hin1I 2960 GGCGCC G[GA]^CG[CT]C 5643 GGCGCC KasI 2960 GGCGCC G^GCGCC 5643 GGCGCC NarI 2960 GGCGCC GG^CGCC 5643 GGCGCC NcoI 2115 CCATGG C^CATGG 2266 CCATGG NspI 4552 GCATGC [GA]CATG^[CT] 4802 ACATGT PinAI 217 ACCGGT A^CCGGT 2601 ACCGGT SacI 2207 GAGCTC GAGCT^C 5867 GAGCTC SexAI 1462 ACCAGGT A^CC[AT]GGT 4750 ACCTGGT SfoI 2960 GGCGCC GGC^GCC 5643 GGCGCC SgrAI 216 CACCGGTG C[GA]^CCGG[CT]G 2600 CACCGGTG SmaI 67 CCCGGG CCC^GGG 3516 CCCGGG StuI 3658 AGGCCT AGG^CCT 5667 AGGCCT XmaCI 67 CCCGGG C^CCGGG 3516 CCCGGG XmaI 67 CCCGGG C^CCGGG 3516 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 133 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI AspI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaI BsaJI BsaWI BseRI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspHI BspMI BsrI BssKI BssSI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DraIII DrdI DsaI EaeI EagI EarI EclXI Eco52I EcoO109I EcoO65I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflFI PleI PstI PvuII RcaI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XcmI XhoII XmnI