##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI Aor51HI AscI AseI
AsnI Asp718I AviII AvrII BbrPI
BbvCI BcgI BclI BfrI BglII
BlnI BsaAI BsaWI BseAI BsiWI
BspDI BspEI BspHI BspLU11I Bst1107I
BstBI BstZ17I ClaI CpoI Eco47III
EcoT22I FbaI FspI HincII HindII
HindIII HpaI KpnI MfeI MluI
MroI MunI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SalI
SbfI ScaI SfuI SnaBI SpeI
Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AccI 5635 GTCTAC GT^[AC][GT]AC
AhdI 4601 GACAGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4798 GTGCAC G^TGCAC
AocI 5585 CCTCAGG CC^T[ACGT]AGG
ApaLI 4798 GTGCAC G^TGCAC
Asp700I 3746 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4601 GACAGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 571 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BlpI 2730 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2730 GCTCAGC GC^T[ACGT]AGC
Bsp1407I 1848 TGTACA T^GTACA
BsrDI 5368 GCAATG GCAATG(2/0)
BsrGI 1848 TGTACA T^GTACA
BssSI 5968 CACGAG CACGAG(-5/-1)
Bsu36I 5585 CCTCAGG CC^T[ACGT]AGG
CelII 2730 GCTCAGC GC^T[ACGT]AGC
DraIII 4231 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 4601 GACAGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 5585 CCTCAGG CC^T[ACGT]AGG
EcoNI 558 CCTGAGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 748 GAATTC G^AATTC
EcoRV 937 GATATC GAT^ATC
PaeR7I 5933 CTCGAG C^TCGAG
PflFI 571 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 642 CTGCAG CTGCA^G
SgrAI 288 CGCCGGCG C[GA]^CCGG[CT]G
SphI 3190 GCATGC GCATG^C
SspBI 1848 TGTACA T^GTACA
SspI 6357 AATATT AAT^ATT
Tth111I 571 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 5933 CTCGAG C^TCGAG
XmnI 3746 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
BbsI 3697 GAAGAC GAAGAC(2/6)
1876 GTCTTC
BpuAI 3697 GAAGAC GAAGAC(2/6)
1876 GTCTTC
BsaBI 1368 GATGGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1578 GATGGAGATC
BsgI 5832 CTGCAC GTGCAG(16/14)
2626 GTGCAG
BsiEI 268 CGGCCG CG[GA][CT]^CG
989 CGGCCG
BstAPI 1467 GCAAAAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5725 GCAAGCCCTGC
EagI 268 CGGCCG C^GGCCG
989 CGGCCG
EclXI 268 CGGCCG C^GGCCG
989 CGGCCG
Eco52I 268 CGGCCG C^GGCCG
989 CGGCCG
MamI 1368 GATGGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1578 GATGGAGATC
NspI 1001 GCATGT [GA]CATG^[CT]
3190 GCATGC
PvuII 5233 CAGCTG CAG^CTG
5327 CAGCTG
SapI 1765 GAAGAGC GCTCTTC(1/4)
4308 GCTCTTC
SfcI 642 CTGCAG C^T[GA][CT]AG
6137 CTATAG
XbaI 5333 TCTAGA T^CTAGA
6184 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII BalI BamHI
BanI BanII BbeI BbvI BciVI
BcnI BfaI BglI BmyI BpmI
BsaHI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssHII BssKI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DrdI
DsaI EaeI EarI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
FseI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NdeII
NgoMIV NlaIII NlaIV PflMI PleI
PpuMI PshAI RsaI SacI SacII
Sau3AI Sau96I ScrFI SexAI SfaNI
SfiI SfoI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI