##################################
# NO CUT ENZYMES (   77 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AgeI        Aor51HI     ApaI        AscI        AseI        
AsnI        Asp718I     AspI        AviII       AvrII       
BbvCI       BfrI        BglI        BlnI        BsaWI       
BseAI       BsiEI       BsiWI       BspDI       BspEI       
BsrBI       BsrFI       BssHII      Bst1107I    BstAPI      
BstBI       BstXI       BstZ17I     Cfr10I      ClaI        
CpoI        DraIII      DrdI        EagI        EclXI       
Eco47III    Eco52I      EcoRV       FauI        FseI        
FspI        KpnI        KspI        MluI        MroI        
NaeI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacII       SalI        SbfI        SfiI        SfuI        
SgrAI       SnaBI       SphI        Sse8387I    SwaI        
Tth111I     XhoI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccII        2026  CGCG                 CG^CG
AcyI         4990  GGCGCC               G[GA]^CG[CT]C
AhdI         2229  GACCCTTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        416  GTGCAC               G^TGCAC
AocI         1273  CCTCAGG              CC^T[ACGT]AGG
ApaLI         416  GTGCAC               G^TGCAC
Asp700I      6025  GAAGGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        2229  GACCCTTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI         4990  GGCGCC               G^G[CT][GA]CC
BbeI         4990  GGCGCC               GGCGC^C
BbrPI        5754  CACGTG               CAC^GTG
BcgI         6465  CGACACTTGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII        1155  AGATCT               A^GATCT
BsaHI        4990  GGCGCC               G[GA]^CG[CT]C
BsmBI        5149  CGTCTC               CGTCTC(1/5)
BspHI        2004  TCATGA               T^CATGA
BssSI        3409  CTCGTG               CACGAG(-5/-1)
BstUI        2026  CGCG                 CG^CG
Bsu36I       1273  CCTCAGG              CC^T[ACGT]AGG
DsaI         2133  CCATGG               C^C[GA][CT]GG
Eam1105I     2229  GACCCTTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       1273  CCTCAGG              CC^T[ACGT]AGG
HaeII        4990  GGCGCC               [GA]GCGC^[CT]
HgaI          551  GACGC                GACGC(5/10)
Hin1I        4990  GGCGCC               G[GA]^CG[CT]C
KasI         4990  GGCGCC               G^GCGCC
MfeI         3362  CAATTG               C^AATTG
MunI         3362  CAATTG               C^AATTG
MvnI         2026  CGCG                 CG^CG
NarI         4990  GGCGCC               GG^CGCC
NcoI         2133  CCATGG               C^CATGG
PmaCI        5754  CACGTG               CAC^GTG
PmeI         2712  GTTTAAAC             GTTT^AAAC
PmlI         5754  CACGTG               CAC^GTG
RcaI         2004  TCATGA               T^CATGA
ScaI         3684  AGTACT               AGT^ACT
SexAI         480  ACCAGGT              A^CC[AT]GGT
SfoI         4990  GGCGCC               GGC^GCC
SpeI         5741  ACTAGT               A^CTAGT
SspI         1468  AATATT               AAT^ATT
XbaI          456  TCTAGA               T^CTAGA
XmnI         6025  GAAGGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccI         2138  GTAGAC               GT^[AC][GT]AC
             6548  GTAGAC              
BamHI        1854  GGATCC               G^GATCC
             3830  GGATCC              
BciVI        2058  GGATAC               GTATCC(6/5)
             4461  GTATCC              
BclI         2007  TGATCA               T^GATCA
             5784  TGATCA              
BlpI          356  GCTGAGC              GC^T[ACGT]AGC
             3846  GCTCAGC             
Bpu1102I      356  GCTGAGC              GC^T[ACGT]AGC
             3846  GCTCAGC             
BsaAI        3290  TACGTG               [CT]AC^GT[GA]
             5754  CACGTG              
BsaBI        1483  GATGTACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2038  GATGGAGATC          
Bsp1407I     1485  TGTACA               T^GTACA
             6238  TGTACA              
BspLU11I      858  ACATGT               A^CATGT
             1760  ACATGT              
BsrGI        1485  TGTACA               T^GTACA
             6238  TGTACA              
CelII         356  GCTGAGC              GC^T[ACGT]AGC
             3846  GCTCAGC             
CfoI         2025  GCGC                 GCG^C
             4991  GCGC                
DraI         2713  TTTAAA               TTT^AAA
             3648  TTTAAA              
FbaI         2007  TGATCA               T^GATCA
             5784  TGATCA              
HhaI         2025  GCGC                 GCG^C
             4991  GCGC                
HinP1I       2025  GCGC                 G^CGC
             4991  GCGC                
HpaI         1051  GTTAAC               GTT^AAC
             4830  GTTAAC              
MamI         1483  GATGTACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2038  GATGGAGATC          
NdeI         2369  CATATG               CA^TATG
             4471  CATATG              
NspI          858  ACATGT               [GA]CATG^[CT]
             1760  ACATGT              
PflMI        1036  CCAAAGTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2741  CCATGATTTGG         
SmaI         1722  CCCGGG               CCC^GGG
             2182  CCCGGG              
SmlI         2917  CTTGAG               C^T[CT][GA]AG
             6379  CTTGAG              
SspBI        1485  TGTACA               T^GTACA
             6238  TGTACA              
TaqI          620  TCGA                 T^CGA
             4662  TCGA                
TthHB8I       620  TCGA                 T^CGA
             4662  TCGA                
Van91I       1036  CCAAAGTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2741  CCATGATTTGG         
XcmI          367  CCAAGTGAATGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1353  CCAGGATAGGCATGG     
XmaCI        1722  CCCGGG               C^CCGGG
             2182  CCCGGG              
XmaI         1722  CCCGGG               C^CCGGG
             2182  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  108 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        AlwNI       ApoI        AspHI       AvaI        
AvaII       BalI        BanII       BbsI        BbvI        
BcnI        BfaI        BmyI        BpmI        BpuAI       
BsaI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
BsoBI       Bsp1286I    BspMI       BsrDI       BsrI        
BssKI       BstEII      BstF5I      BstNI       BstPI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
DraII       EaeI        EarI        EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRII      EcoT14I     EcoT22I     
Fnu4HI      FokI        HaeIII      HapII       HincII      
HindII      HindIII     HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NsiI        PleI        PpuMI       PstI        
PvuII       RsaI        SacI        SapI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StuI        
StyI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       XhoII