##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI Aor51HI ApaI AscI AseI
AsnI Asp718I AspI AviII AvrII
BbvCI BfrI BglI BlnI BsaWI
BseAI BsiEI BsiWI BspDI BspEI
BsrBI BsrFI BssHII Bst1107I BstAPI
BstBI BstXI BstZ17I Cfr10I ClaI
CpoI DraIII DrdI EagI EclXI
Eco47III Eco52I EcoRV FauI FseI
FspI KpnI KspI MluI MroI
NaeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI SfiI SfuI
SgrAI SnaBI SphI Sse8387I SwaI
Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccII 2026 CGCG CG^CG
AcyI 4990 GGCGCC G[GA]^CG[CT]C
AhdI 2229 GACCCTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 416 GTGCAC G^TGCAC
AocI 1273 CCTCAGG CC^T[ACGT]AGG
ApaLI 416 GTGCAC G^TGCAC
Asp700I 6025 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2229 GACCCTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 4990 GGCGCC G^G[CT][GA]CC
BbeI 4990 GGCGCC GGCGC^C
BbrPI 5754 CACGTG CAC^GTG
BcgI 6465 CGACACTTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1155 AGATCT A^GATCT
BsaHI 4990 GGCGCC G[GA]^CG[CT]C
BsmBI 5149 CGTCTC CGTCTC(1/5)
BspHI 2004 TCATGA T^CATGA
BssSI 3409 CTCGTG CACGAG(-5/-1)
BstUI 2026 CGCG CG^CG
Bsu36I 1273 CCTCAGG CC^T[ACGT]AGG
DsaI 2133 CCATGG C^C[GA][CT]GG
Eam1105I 2229 GACCCTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1273 CCTCAGG CC^T[ACGT]AGG
HaeII 4990 GGCGCC [GA]GCGC^[CT]
HgaI 551 GACGC GACGC(5/10)
Hin1I 4990 GGCGCC G[GA]^CG[CT]C
KasI 4990 GGCGCC G^GCGCC
MfeI 3362 CAATTG C^AATTG
MunI 3362 CAATTG C^AATTG
MvnI 2026 CGCG CG^CG
NarI 4990 GGCGCC GG^CGCC
NcoI 2133 CCATGG C^CATGG
PmaCI 5754 CACGTG CAC^GTG
PmeI 2712 GTTTAAAC GTTT^AAAC
PmlI 5754 CACGTG CAC^GTG
RcaI 2004 TCATGA T^CATGA
ScaI 3684 AGTACT AGT^ACT
SexAI 480 ACCAGGT A^CC[AT]GGT
SfoI 4990 GGCGCC GGC^GCC
SpeI 5741 ACTAGT A^CTAGT
SspI 1468 AATATT AAT^ATT
XbaI 456 TCTAGA T^CTAGA
XmnI 6025 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 2138 GTAGAC GT^[AC][GT]AC
6548 GTAGAC
BamHI 1854 GGATCC G^GATCC
3830 GGATCC
BciVI 2058 GGATAC GTATCC(6/5)
4461 GTATCC
BclI 2007 TGATCA T^GATCA
5784 TGATCA
BlpI 356 GCTGAGC GC^T[ACGT]AGC
3846 GCTCAGC
Bpu1102I 356 GCTGAGC GC^T[ACGT]AGC
3846 GCTCAGC
BsaAI 3290 TACGTG [CT]AC^GT[GA]
5754 CACGTG
BsaBI 1483 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2038 GATGGAGATC
Bsp1407I 1485 TGTACA T^GTACA
6238 TGTACA
BspLU11I 858 ACATGT A^CATGT
1760 ACATGT
BsrGI 1485 TGTACA T^GTACA
6238 TGTACA
CelII 356 GCTGAGC GC^T[ACGT]AGC
3846 GCTCAGC
CfoI 2025 GCGC GCG^C
4991 GCGC
DraI 2713 TTTAAA TTT^AAA
3648 TTTAAA
FbaI 2007 TGATCA T^GATCA
5784 TGATCA
HhaI 2025 GCGC GCG^C
4991 GCGC
HinP1I 2025 GCGC G^CGC
4991 GCGC
HpaI 1051 GTTAAC GTT^AAC
4830 GTTAAC
MamI 1483 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2038 GATGGAGATC
NdeI 2369 CATATG CA^TATG
4471 CATATG
NspI 858 ACATGT [GA]CATG^[CT]
1760 ACATGT
PflMI 1036 CCAAAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2741 CCATGATTTGG
SmaI 1722 CCCGGG CCC^GGG
2182 CCCGGG
SmlI 2917 CTTGAG C^T[CT][GA]AG
6379 CTTGAG
SspBI 1485 TGTACA T^GTACA
6238 TGTACA
TaqI 620 TCGA T^CGA
4662 TCGA
TthHB8I 620 TCGA T^CGA
4662 TCGA
Van91I 1036 CCAAAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2741 CCATGATTTGG
XcmI 367 CCAAGTGAATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1353 CCAGGATAGGCATGG
XmaCI 1722 CCCGGG C^CCGGG
2182 CCCGGG
XmaI 1722 CCCGGG C^CCGGG
2182 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BalI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrDI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EaeI EarI EcoNI EcoO109I
EcoO65I EcoRI EcoRII EcoT14I EcoT22I
Fnu4HI FokI HaeIII HapII HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NsiI PleI PpuMI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII