##################################
# NO CUT ENZYMES ( 45 enzymes) #
##################################
AatII AhdI Aor51HI AscI AspEI
AspI AvrII BbeI BbrPI BlnI
BsaBI BsiWI BspHI BsrDI BssSI
CpoI Eam1105I Eco47III EcoRV HpaI
KasI MamI NarI NruI PacI
PaeR7I PflFI PmaCI PmlI PshAI
PvuI RcaI RsrII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SgrAI Sse8387I Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 563 GGTACC G^GTACC
AclI 4879 AACGTT AA^CGTT
AflII 4236 CTTAAG C^TTAAG
AgeI 1210 ACCGGT A^CCGGT
AocI 1645 CCTCAGG CC^T[ACGT]AGG
AseI 4936 ATTAAT AT^TAAT
AsnI 4936 ATTAAT AT^TAAT
Asp718I 563 GGTACC G^GTACC
AviII 2024 TGCGCA TGC^GCA
BamHI 822 GGATCC G^GATCC
BbvCI 6369 GCTGAGG CCTCAGC(-5/-2)
BclI 179 TGATCA T^GATCA
BfrI 4236 CTTAAG C^TTAAG
BglII 2891 AGATCT A^GATCT
BlpI 250 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 250 GCTGAGC GC^T[ACGT]AGC
Bsp1407I 2582 TGTACA T^GTACA
BsrGI 2582 TGTACA T^GTACA
BstAPI 2905 GCACCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 6397 TTCGAA TT^CGAA
BstEII 2662 GGTCACC G^GT[ACGT]ACC
BstPI 2662 GGTCACC G^GT[ACGT]ACC
BstXI 5769 CCAGGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1645 CCTCAGG CC^T[ACGT]AGG
CelII 250 GCTGAGC GC^T[ACGT]AGC
DrdI 4744 GACTGTGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1645 CCTCAGG CC^T[ACGT]AGG
EcoO65I 2662 GGTCACC G^GT[ACGT]ACC
EcoT22I 3160 ATGCAT ATGCA^T
FbaI 179 TGATCA T^GATCA
FseI 658 GGCCGGCC GGCCGG^CC
FspI 2024 TGCGCA TGC^GCA
KpnI 563 GGTACC GGTAC^C
KspI 727 CCGCGG CCGC^GG
MfeI 4451 CAATTG C^AATTG
MluI 1583 ACGCGT A^CGCGT
MunI 4451 CAATTG C^AATTG
NotI 506 GCGGCCGC GC^GGCCGC
NsiI 3160 ATGCAT ATGCA^T
NspV 6397 TTCGAA TT^CGAA
PinAI 1210 ACCGGT A^CCGGT
PmeI 2213 GTTTAAAC GTTT^AAAC
PshBI 4936 ATTAAT AT^TAAT
Psp1406I 4879 AACGTT AA^CGTT
SacII 727 CCGCGG CCGC^GG
SfuI 6397 TTCGAA TT^CGAA
SmaI 497 CCCGGG CCC^GGG
SphI 5735 GCATGC GCATG^C
SspBI 2582 TGTACA T^GTACA
StuI 2566 AGGCCT AGG^CCT
SwaI 2069 ATTTAAAT ATTT^AAAT
XmaCI 497 CCCGGG C^CCGGG
XmaI 497 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 543 GGCGTC G[GA]^CG[CT]C
1118 GACGCC
Alw44I 1306 GTGCAC G^TGCAC
1756 GTGCAC
ApaLI 1306 GTGCAC G^TGCAC
1756 GTGCAC
Asp700I 2319 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3643 GAAATACTTC
BcgI 6322 CGATTAGAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2726 GCAACAATATCG
BciVI 4089 GGATAC GTATCC(6/5)
6208 GGATAC
BsaHI 543 GGCGTC G[GA]^CG[CT]C
1118 GACGCC
BsiEI 507 CGGCCG CG[GA][CT]^CG
661 CGGCCG
BsmBI 3502 CGTCTC CGTCTC(1/5)
750 GAGACG
BspDI 3362 ATCGAT AT^CGAT
5110 ATCGAT
BspLU11I 4681 ACATGT A^CATGT
4921 ACATGT
Bst1107I 4968 GTATAC GTA^TAC
6513 GTATAC
BstZ17I 4968 GTATAC GTA^TAC
6513 GTATAC
ClaI 3362 ATCGAT AT^CGAT
5110 ATCGAT
DraI 2070 TTTAAA TTT^AAA
2214 TTTAAA
DraIII 2586 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
5608 CACATTGTG
EagI 507 CGGCCG C^GGCCG
661 CGGCCG
EclXI 507 CGGCCG C^GGCCG
661 CGGCCG
Eco52I 507 CGGCCG C^GGCCG
661 CGGCCG
EcoNI 1944 CCTGAACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5478 CCTGTATAAGG
EcoRI 591 GAATTC G^AATTC
2937 GAATTC
Hin1I 543 GGCGTC G[GA]^CG[CT]C
1118 GACGCC
MslI 4384 CATGGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
6088 CATGGAGGTG
NheI 1784 GCTAGC G^CTAGC
3207 GCTAGC
PflMI 3993 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5607 CCACATTGTGG
SacI 1669 GAGCTC GAGCT^C
5285 GAGCTC
SmlI 3199 CTCAAG C^T[CT][GA]AG
4236 CTTAAG
SnaBI 3566 TACGTA TAC^GTA
5089 TACGTA
SpeI 638 ACTAGT A^CTAGT
1022 ACTAGT
SspI 4627 AATATT AAT^ATT
6558 AATATT
Van91I 3993 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5607 CCACATTGTGG
XmnI 2319 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3643 GAAATACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccI AccII AccIII AciI AcsI
AfaI AflIII AluI AlwI AlwNI
ApaI ApoI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaAI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspEI BspMI BsrBI BsrFI
BsrI BssHII BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MroI MscI MseI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeI NdeII NgoMIV NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII